Radiation oncology and radiotherapy. Part A /

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Sato, Ai (Editor )
Formato: eBook
Idioma:Inglés
Publicado: Cambridge, MA : Academic Press, 2022.
Colección:Methods in cell biology ; v. 172
Temas:
Radiotherapy > Technological innovations.
Cancer > Radiotherapy > Technological innovations.
Radiotherapy.
Cancer > Radiotherapy.
Radiotherapy
Radiation Oncology
Radioth�erapie > Innovations.
Cancer > Radioth�erapie > Innovations.
Radioth�erapie.
Cancer > Radioth�erapie.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Intro
  • Radiation Oncology and Radiotherapy Part A
  • Copyright
  • Contents
  • Contributors
  • Radiation therapy: An old dog learning new tricks
  • Acknowledgments
  • Conflict of interest
  • References
  • Chapter 1: Cytofluorometric assessment of cell cycle progression in irradiated cells
  • 1. Introduction
  • 2. Materials
  • 2.1. Common disposables
  • 2.2. Cells and reagents
  • 2.3. Equipment
  • 2.4. Software
  • 3. Methods
  • 3.1. Cell seeding and treatment (see Note 17)
  • 3.2. BrdU pulsing
  • 3.3. Fixation, permeabilization and DNA denaturation
  • 3.4. Staining
  • 3.5. Flow cytometry acquisition
  • 3.6. Data analysis
  • 4. Notes
  • 5. Concluding remarks
  • Acknowledgments
  • Competing interests
  • References
  • Chapter 2: Cytofluorometric assessment of acute cell death responses driven by radiation therapy
  • 1. Introduction
  • 2. Materials
  • 2.1. Common disposables
  • 2.2. Cells and reagents
  • 2.3. Equipment
  • 2.4. Software
  • 3. Methods
  • 3.1. Cell culture and treatments (see Note 21)
  • 3.2. Sample collection
  • 3.3. DiOC6(3)/PI co-staining
  • 3.4. FITC-AnnV/PI co-staining
  • 3.5. Acquisition
  • 3.6. Gating and data analysis
  • 4. Notes
  • 5. Concluding remarks
  • Acknowledgments
  • Conflicts of interest
  • References
  • Chapter 3: Assessment of lipid peroxidation in irradiated cells
  • 1. Introduction
  • 2. Materials
  • 2.1. Disposables
  • 2.2. Reagents
  • 2.3. Cell lines
  • 2.4. Equipment
  • 2.5. Software
  • 3. Methods
  • 3.1. Cell cultures
  • 3.2. Cell irradiation
  • 3.3. BODIPY 581/591 C11 staining
  • 3.4. Flow cytometry
  • 3.5. Data analysis
  • 3.6. Concluding remarks
  • 3.7. Notes
  • Acknowledgments
  • Disclosures
  • References
  • Chapter 4: Biochemical, genomic, and epigenomic profiling of isolated cancer cell lines micronuclei
  • 1. Introduction
  • 2. Materials (see note 1).
  • 2. Material
  • 2.1. Disposables
  • 2.2. Equipment
  • 2.3. Reagents
  • 2.4. Biological material
  • 3. Method
  • 3.1. Cell culture
  • 3.2. Compound preparation
  • 3.3. Cell seeding and treatment
  • 3.4. Image acquisition and analysis
  • 3.5. Cell features extraction and data analysis
  • 4. Notes
  • 5. Concluding remarks
  • Acknowledgments
  • Conflict of interest
  • References
  • Chapter 10: RT-PCR-assisted quantification of type I IFN responses in irradiated cancer cells
  • 1. Introduction
  • 2. Materials
  • 2.1. Common disposables
  • 2.2. Cells and reagents
  • 2.3. Equipment
  • 3. Methods
  • 3.1. Cell culture and treatments (see Note 9)
  • 3.2. RNA extraction (see Note 19)
  • 3.3. RT-PCR (see Note 32)
  • 3.4. Data analysis
  • 4. Notes
  • 5. Concluding remarks
  • Acknowledgments
  • Competing interests
  • References
  • Chapter 11: ELISA-based quantification of type I IFN secretion by irradiated cancer cells
  • 1. Introduction
  • 2. Material
  • 2.1. Disposables
  • 2.2. Cells and reagents
  • 2.3. Equipment
  • 2.4. Software
  • 3. Method
  • 3.1. Cell culture, treatment and sample collection
  • 3.2. Enzyme-linked immunosorbent assay (ELISA)
  • 3.2.1. Reagent preparation
  • 3.2.2. Procedure
  • 3.2.3. Data analysis
  • 3.3. Bead-based multiplex immunoassays
  • 3.3.1. Reagent preparation
  • 3.3.2. Procedure
  • 3.3.3. Data acquisition with a flow cytometer and analysis
  • 4. Concluding remarks
  • Conflict of interest statement
  • References
  • Chapter 12: Assessment of hypoxia by pimonidazole staining following radiotherapy
  • 1. Introduction
  • 1.1. Hypoxia in tumors
  • 1.2. Methods to measure hypoxia
  • 1.3. Pimonidazole HCl
  • 2. Materials
  • 2.1. Common disposables
  • 2.2. Equipment
  • 2.3. Reagents
  • 3. Methods
  • 3.1. Establishment of B16F10 melanoma tumors and radiotherapy treatment.
  • 3.2. Pimonidazole administration, tumor collection and OCT preservation
  • 3.3. Pimonidazole staining and fluorescence microscopy
  • 4. Notes
  • 5. Concluding remarks
  • Disclosures
  • References.

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