Advances in clinical chemistry. Volume 108 /

Advances in Clinical Chemistry, Volume 108, the latest installment in this internationally acclaimed series, contains chapters authored by world-renowned clinical laboratory scientists, physicians and research scientists. The serial discusses the latest and most up-to-date technologies related to th...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Makowski, Gregory S. (Gregory Stephen) (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam : Academic Press, 2022.
Temas:
Clinical chemistry.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Chapter Three: Testosterone analysis in prostate cancer patients
  • 1. Introduction
  • 2. Gonadal testosterone regulation
  • 3. Evolution of androgen deprivation therapy
  • 3.1. Surgical intervention and estrogen administration
  • 3.2. LHRH agonists and antagonists
  • 3.3. AR inhibitors
  • 3.4. CYP17A1 inhibition
  • 3.5. Bipolar androgen therapy
  • 4. Testosterone analysis
  • 4.1. Early testosterone analysis
  • 4.2. Contemporary testosterone analysis
  • 4.2.1. Automated IA
  • 4.2.2. Mass spectrometry-based assays
  • 5. Automated IA vs LC-MS/MS assays
  • 6. Potential clinical utility of ultra-sensitive testosterone assays
  • 7. Conclusion
  • References
  • Chapter Four: Extracellular vesicles in respiratory disease
  • 1. Introduction
  • 1.1. Extracellular vesicles
  • 1.2. Types of EVs
  • 1.3. General functions of EVs
  • 2. Experimental techniques for EV research
  • 2.1. Methods for EV isolation
  • 2.1.1. Differential centrifugation
  • 2.1.2. Density gradient centrifugation
  • 2.1.3. Size exclusion chromatography
  • 2.1.4. Commercial kits for polymer precipitation
  • 2.1.5. Precipitation with chemicals
  • 2.1.6. Immunoprecipitation
  • 2.1.7. Ultrafiltration
  • 2.1.8. Microfluidic technologies
  • 2.2. Methods for EV characterization
  • 2.2.1. Dynamic light scattering
  • 2.2.2. Nanoparticle tracking analysis
  • 2.2.3. Nanoscale flow cytometry
  • 2.2.4. Transmission electron microscopy
  • 3. EVs in the respiratory system
  • 3.1. EVs from BALF
  • 3.2. Biological significance of EVs in common respiratory diseases
  • 3.2.1. Acute lung injury and acute respiratory distress syndrome
  • 3.2.2. Chronic obstructive pulmonary disease
  • 3.2.3. Pulmonary arterial hypertension
  • 3.2.4. Idiopathic pulmonary fibrosis
  • 3.2.5. Asthma
  • 3.3. Clinical applications of respiratory EVs
  • 3.3.1. Respiratory EV-miRNAs as diagnostic markers.

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