Applied environmental metabolomics : community insights and guidance from the field /

And future perspectives -- References -- Chapter 14 Exploring the coral bleaching tipping point with 13 C metabolomics  -- Scope -- Aims and objectives -- Introduction -- Approach (material and methods) -- Biological sample -- Environment sampled -- Sampling protocol -- Analytical protocol -- Main...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Beale, David J. (David John)
Formato: Electrónico eBook
Idioma:Inglés
Publicado: London, UK ; San Diego, CA : Academic Press, [2022]
Temas:
Metabolites > Environmental aspects.
Metabolism > Environmental aspects.
Ecology.
M�etabolites > Aspect de l'environnement.
Ecology
Metabolites > Environmental aspects
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Intro
  • Applied Environmental Metabolomics: Community insights and guidance from the field
  • Copyright
  • Contents
  • Contributors
  • Foreword
  • Part 1 Introduction
  • Chapter 1 Applied environmental metabolomics: Eliciting viewpoints from the metabolomics research community
  • Introduction
  • Reports from the field
  • Case study contributions
  • The future
  • References
  • Part 2 Reports from the field
  • Chapter 2 Measuring root exudate metabolites in holm oak (Quercus ilex) under drought and recovery
  • Scope
  • Aims and objectives
  • Introduction
  • Approach (materials and methods)
  • Biological sample
  • Environment sampled
  • Exudate sampling protocol
  • Main findings
  • Summary of project outcomes
  • Concerns and recommendations
  • Summary and future perspectives
  • References
  • Chapter 3 Visualization of cyanogenic glycosides in floral tissues
  • Scope
  • Aims and objectives of the study
  • Introduction
  • Approach (material and methods)
  • Biological samples
  • Sampling protocol
  • Analytical protocol
  • Quantitative determination of cyanogenesis
  • Extraction and identification of cyanogenic glycosides
  • MALDI mass spectrometry imaging
  • Main findings
  • Quantification of floral cyanogenic glycoside concentrations
  • Identification of cyanogenic glycosides
  • Detection of cyanogenic glycosides in Proteaceae flowers using MALDI-MSI
  • What went right
  • What went wrong
  • Suggestions for improvements for future studies
  • Final remarks
  • Acknowledgments
  • References
  • Chapter 4 Environmental assessment of metal impacted soils using community metabolic profiling 
  • Scope
  • Aims and objectives
  • Introduction
  • Approach (materials and methods)
  • Site description
  • Soil analysis
  • Metabolite extraction
  • 1 H NMR analysis
  • GC-MS analysis
  • Statistical analysis
  • Main findings
  • Soil analysis
  • 1 H Nmr
  • Gc-Ms.
  • Multivariate analysis
  • Discussion
  • What went right?
  • What could be improved?
  • Labour intensive sample analysis
  • Sample matrix
  • Metabolite ID
  • Integration
  • Final thoughts
  • References
  • Chapter 5 Decoding the metabolic landscape of maize responses to experimentally controlled drought stress: A greenhouse case
  • Scope
  • Aims and objectives
  • Introduction
  • Approach (material and methods)
  • Biological samples: Plant cultivation and treatments
  • Sampling protocol: Phenotypic and physiological evaluation, harvesting plant materials and metabolite extraction
  • Analytical protocol
  • Metabolomics data acquisition: LC-MS-based untargeted and targeted analyses
  • Data mining: Data processing and chemometrics
  • Metabolite annotation for untargeted metabolomics data
  • Main findings
  • Metabolic responses to drought: Dynamic changes in both primary and secondary metabolism
  • Global reprogramming of the maize metabolism in response to drought stress spans several metabolic pathways
  • Concluding remarks-"What went right" and "what went wrong"
  • Acknowledgments
  • Conflict of interest
  • References
  • Chapter 6 Nontargeted screening of metabolites to discriminate disease suppressive and nonsuppressive soils for the fungal ...
  • Scope
  • Aims
  • Introduction
  • Approach
  • Biological sample
  • Soil sampling protocol
  • Environment sampled
  • Analytical protocol
  • Main findings
  • What went right?
  • Site selection
  • Soil sampling strategy
  • Pilot studies
  • What went wrong?
  • Different cereal crops sown in the suppressive and nonsuppressive fields
  • Lack of suitable databases for annotation
  • Topography
  • Recommendations for agricultural field studies
  • Final remarks
  • References
  • Chapter 7 Ecosystem metabolomics of dissolved organic matter from arctic soil pore water across seasonal transitions 
  • Scope.
  • Aims and objectives
  • Introduction
  • Approach
  • Environment sampled
  • Sampling protocol
  • Analytical protocol
  • Main findings
  • Biogeochemical variables
  • Soil porewater metabolome
  • What went right
  • What went wrong
  • Conclusion
  • References
  • Chapter 8 Temporal trends in metabolite profiles correspond with seasonal patterns of temperature and rainfall during field...
  • Scope
  • Aims and objectives of the study
  • Introduction
  • Approach (materials and methods)
  • Biological samples
  • Environment sampled
  • Sampling protocol
  • Analytical protocol
  • Main findings
  • What went right?
  • What would you recommend to other researchers wanting to do similar research?
  • What could be improved?
  • Final thoughts
  • References
  • Chapter 9 Metabolic responses of eelgrass (Zostera marina) to artificially induced stresses
  • Scope
  • Aims and objectives
  • Introduction
  • Approach (material and methods)
  • Biological samples
  • Environment sampled
  • Sampling protocol
  • Herbicide exposure
  • Low light (dark) and high temperature
  • Growth, photosynthetic efficiency
  • Analytical protocol
  • Main findings
  • Physiological effects on eelgrass
  • Metabolic fluctuations associated with ambient stressors
  • What went right
  • What went wrong
  • Summary and future perspectives
  • References
  • Chapter 10 Metabolomic profiling of anthropogenically threatened Australian seagrass Zostera muelleri using one- and two-di...
  • Scope
  • Aims and objectives
  • Introduction
  • Experimental methods
  • Seagrass sample collection
  • Sample preparation
  • Metabolite profiling
  • Data preprocess and analysis
  • Main findings
  • What went wrong in seagrass metabolomics
  • Leaf age and lifespan influence metabolite profiling
  • Need for marine plants-based MS libraries
  • What went right in seagrass metabolomics
  • Detection of specific classes of compounds.
  • Detection of phenylpropenoid compounds and aromatic amino acids
  • Detection of unique sugars, their derivatives, and phytohormones
  • Summary and future perspectives
  • Acknowledgment
  • References
  • Chapter 11 The metabolic response of marine copepods (Calanus spp.) to food deprivation, end-of-century ocean acidificatio...
  • Scope
  • Aims and objectives of the study
  • Introduction
  • Approach (material and methods)
  • Biological sample
  • Environment sampled
  • Sampling protocol
  • Analytical protocol
  • Main findings
  • What went right?
  • What went wrong?
  • Summary and future perspectives
  • Acknowledgments
  • References
  • Further reading
  • Chapter 12 Untargeted screening of xenobiotics and metabolic profiles of green sea turtles on the Great Barrier Reef
  • Scope
  • Aims and objectives
  • Introduction
  • Approach (material and methods)
  • Biological sample
  • Environment sampled
  • Sampling protocol
  • Analytical protocol
  • Main findings
  • Evidence of oxidative stress in Upstart Bay turtles
  • Evidence of anthropogenic influence in Cleveland Bay
  • What went right
  • Unique biological samples
  • Data-rich regional profile
  • Case-control sampling strategy
  • What went wrong
  • Labor-intensive data analysis
  • Noncomprehensive sample and data analysis
  • Delayed response to catastrophic event
  • Suggestions for future studies
  • Summary and future perspectives
  • References
  • Chapter 13 Butterflyfish gill mucus metabolome reflects diet preferences and gill parasite intensities
  • Scope
  • Aims and objectives
  • Introduction
  • Approach (materials and methods)
  • Biological sample
  • Environment sampled
  • Sampling protocol
  • Analytical protocol
  • Main findings
  • What went right
  • Large collection of wild biological samples
  • Metabolomic pipeline
  • What went wrong
  • Samples limitations
  • Limited feature annotation.

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