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SCIDIR_on1294317155 |
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OCoLC |
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20231120010631.0 |
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m o d |
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cr |n||||||||| |
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220131s2022 xx o 0|| 0 eng d |
| 040 |
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|a YDX
|b eng
|c YDX
|d OPELS
|d EBLCP
|d OCLCO
|d UKAHL
|d OCLCQ
|d OCLCO
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| 019 |
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|a 1294344908
|a 1295644637
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|a 9780323907361
|q (electronic bk.)
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|a 0323907369
|q (electronic bk.)
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|z 0323907350
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|z 9780323907354
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| 035 |
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|a (OCoLC)1294317155
|z (OCoLC)1294344908
|z (OCoLC)1295644637
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| 050 |
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4 |
|a QD412.S5
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| 082 |
0 |
4 |
|a 572.555
|2 23
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| 245 |
0 |
0 |
|a SELENOPROTEIN STRUCTURE AND FUNCTION
|h [electronic resource].
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| 260 |
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|a [S.l.] :
|b ELSEVIER ACADEMIC PRESS,
|c 2022.
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| 300 |
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|a 1 online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in enzymology ;
|v 662
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| 505 |
0 |
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|a Intro -- Selenoprotein Structure and Function -- Copyright -- Contents -- Contributors -- Preface -- Chapter One: Examining xCT-mediated selenium uptake and selenoprotein production capacity in cells -- 1. Introduction -- 2. Measurement of selenium uptake and selenoprotein production as mediated by xCT transporter -- 2.1. Evaluation of xCT expression of SLC7A11 and SLC3A2 -- 2.1.1. Technical tips for the non-heat-denaturing Western blot of xCT -- 2.2. Measurement of extracellular thiols as readout of xCT function leading to uptake -- 2.2.1. Protocol for Ellman�s test
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| 505 |
8 |
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|a 2.2.1.1. Ellman�s test for measuring xCT activity on extracellular reduction -- 2.2.1.2. Materials and equipment -- 2.2.1.3. Step-by-step method details -- 2.2.1.4. Expected outcomes -- 2.2.1.5. Quantification and statistical analysis -- 2.3. Measurement of selenium uptake -- 2.3.1. Protocol for fluorometric assay -- 2.3.1.1. Measurement of cellular selenium uptake capacity using fluorometric assay -- 2.3.1.2. Materials and equipment -- 2.3.1.3. Step-by-step method details -- 2.3.1.4. Expected outcomes -- 2.3.1.5. Quantification and statistical analysis -- 2.3.1.6. Advantages
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| 505 |
8 |
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|a 2.3.1.7. Safety considerations and standards -- 2.3.2. Protocol for sample prep for ICP-MS analysis -- 2.3.2.1. Measurement of cellular selenium uptake capacity using ICP-MS -- 2.3.2.2. Materials and equipment -- 2.3.2.3. Step-by-step method details -- 2.3.2.4. Example condition for ICP-MS run -- 2.3.2.5. Expected outcomes -- 2.3.2.6. Quantification and statistical analysis -- 2.3.2.7. Advantages -- 2.3.2.8. Limitations -- 2.3.2.9. Safety considerations and standards -- 2.4. Western blotting of selenoproteins as final readout -- 2.4.1. Technical tips for the Western blotting of selenoproteins
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8 |
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|a 3. Summary -- Acknowledgment -- References -- Chapter Two: Methods for accurate and reproducible studies of pharmacological effects of selenium in cancer -- 1. Introduction -- 2. Selenium cytotoxicity in cancer cell lines -- 2.1. Effects of cell culture media composition on IC50 determinations of selenium compounds in cancer cell lines -- 2.2. Phenotype expression depending on cell culture media -- 2.3. Protocol for adherent cells -- 2.3.1. Materials -- 2.3.2. Procedure -- 2.4. Protocol for cells in suspension -- 2.4.1. Preparation of cell blocks for suspension cells
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|a 2.4.2. Materials and equipment -- 2.4.3. Procedure -- 2.5. The activity of the selenoprotein thioredoxin reductase (TXNRD) in cells cultured in different media -- 2.6. The extracellular redox milieu -- 2.7. Thiol measurements -- 2.7.1. Materials -- 2.7.2. Procedure -- 2.7.3. Quantification -- 2.8. Alternative method-Thiol measurement by DTDP -- 2.8.1. Buffers and chemical preparation -- 2.8.2. Materials and equipment -- 2.8.3. Procedure -- 2.8.4. Expected outcomes -- 2.8.5. Quantification -- 2.8.6. Advantage
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| 650 |
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0 |
|a Selenoproteins.
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| 650 |
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2 |
|a Selenoproteins
|0 (DNLM)D051140
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| 650 |
|
6 |
|a S�el�enoprot�eines.
|0 (CaQQLa)000286271
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| 650 |
|
7 |
|a Selenoproteins
|2 fast
|0 (OCoLC)fst01736533
|
| 776 |
0 |
8 |
|i ebook version :
|z 9780323907361
|
| 776 |
0 |
8 |
|c Original
|z 0323907350
|z 9780323907354
|w (OCoLC)1260665606
|
| 830 |
|
0 |
|a Methods in enzymology ;
|v 662.
|
| 856 |
4 |
0 |
|u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/662
|z Texto completo
|
