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|a 1280276655
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|a Crispr genome surgery in stem cells and disease tissues /
|c edited by Stephen H. Tsang.
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| 260 |
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|a London :
|b Academic Press,
|c [2022]
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| 300 |
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|a 1 online resource
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|a text
|b txt
|2 rdacontent
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|a computer
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|a online resource
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|a Front Cover -- CRISPR Genome Surgery in Stem Cells and Disease Tissues -- Copyright Page -- Contents -- List of contributors -- Foreword -- 1 The history of CRISPR: from discovery to the present -- The beginnings of CRISPR/Cas9 -- Underlying mechanisms of CRISPR/Cas9 -- Using CRISPR/Cas9 for mammalian gene editing -- Future directions -- Acknowledgments -- Author contributions -- Conflict of interest statement -- References -- 2 CRISPR-Cas orthologs and variants -- Introduction -- Cas9: a model of CRISPR-Cas systems -- Nickase SpCas9 -- High-fidelity and enhanced SpCas9 -- SpCas9 variants with novel PAM requirements -- Deactivated-Cas9 characterization and application -- FokI-dCas9: a dCas9 application to improve specificity -- Base editing -- CRISPR-Cas orthologs -- CRISPR-Cas9 system orthologs -- Staphylococcus aureus Cas9 -- Streptococcus thermophilus Cas9 -- Neisseria meningitides Cas9 -- Francisella novicida Cas9 -- Campylobacter jejuni Cas9 -- CRISPR-Cas12a (Cpf1) system -- CRISPR-Cas13a (C2c2) system -- Conclusion -- References -- 3 Genome engineering with CRISPR/Cas9, ZFNs, and TALENs -- Introduction -- Zinc-finger nucleases -- Transcription activator-like effector nucleases -- CRIPSR/Cas9 -- Conclusion -- References -- 4 Single-guide RNAs: rationale and design -- CRISPR/Cas9 background and the role of sgRNA -- Design of sgRNA -- Online tools to aid in sgRNA design -- Factors mediating sgRNA targeting specificity -- sgRNA design criteria to optimize specificity and efficiency while minimizing off-target activity -- sgRNA secondary structures impede formation of active Cas9-sgRNA complexes -- sgRNA and Cas9 concentration influences off-target activity -- Strategies to minimize sgRNA off-target activity -- Conclusion -- References.
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| 505 |
8 |
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|a Delivery of CRISPR/Cas9 gene editing components to the cornea -- Summary -- References -- 8 Employing nonhomologous end joining and homology-directed repair for treatment of Leber congenital amaurosis and inherite... -- Leber congenital amaurosis and inherited retinal degeneration -- Genetic aberrations in patients with LCA -- Genetic aberrations in patients with other IRDs -- Genome surgery for treatment of human diseases -- Modes of correction with genome surgery -- Nonhomologous end joining -- Homology-directed repair -- Employing NHEJ and HDR for treatment of LCA and IRD -- Nonhomologous end joining -- Homology-directed repair -- Future direction and conclusions -- References -- 9 The ethics of gene editing in human stem cells -- Overview of legal and political regulation -- Human embryonic stem cells -- Induced pluripotent stem cells -- Germline gene editing -- Looking ahead: the slippery slope -- References -- Index -- Back Cover.
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| 650 |
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|a CRISPR-associated protein 9.
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|a CRISPR-Associated Protein 9
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|a Clustered Regularly Interspaced Short Palindromic Repeats
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|a Prot�eine-9 associ�ee �a CRISPR.
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|a CRISPR (G�en�etique)
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7 |
|a CRISPR-associated protein 9
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7 |
|a CRISPR (Genetics)
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|0 (OCoLC)fst02000514
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1 |
|
|a Tsang, Stephen H.
|
| 776 |
0 |
8 |
|i Print version:
|z 0128178760
|z 9780128178768
|w (OCoLC)1135669118
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| 776 |
0 |
8 |
|i Print version:
|t CRISPR genome surgery in stem cells and disease tissues
|z 9780128178768
|w (OCoLC)1264402161
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| 856 |
4 |
0 |
|u https://sciencedirect.uam.elogim.com/science/book/9780128178768
|z Texto completo
|
