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Advanced methods in molecular biology and biotechnology : a practical lab manual /
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
London :
Academic Press,
[2021]
|
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Intro
- Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual
- Copyright
- Dedication
- Contents
- Preface
- Foreword
- Acknowledgment
- Introduction
- About the author
- Disclaimer
- Chapter 1 Introduction to molecular biology techniques
- Safety measures
- Preparation of the solutions
- Working with the nucleic acids
- Storage
- Purification
- Quantitation
- Concentration
- Restriction enzymes
- Sterile techniques
- References
- Chapter 2 Agarose gel electrophoresis
- Definition
- Rationale
- Before you begin
- Key resources table
- Materials and equipments
- Materials and equipments required
- Reagents required
- Step-by-step method details
- Timing: 1-1.5 h (depending on the size of the sample)
- Expected outcomes
- Quantification and statistical analysis
- Advantages
- Limitations
- Optimization and troubleshooting
- Problem
- Potential solution
- Safety considerations and standards
- Alternative methods/procedures
- References
- Chapter 3 SDS-polyacrylamide gel electrophoresis
- Definition
- Rationale
- Before you begin
- Key resources table
- Materials and equipment
- Step-by-step method details
- Timing: 3-4 h (depends on the plate size)
- Expected outcomes
- Quantification and statistical analysis
- Advantages
- Limitations
- Optimization and troubleshooting
- Problem
- Potential solution
- Safety considerations and standards
- Alternative methods/procedures
- References
- Chapter 4 Immunoelectrophoresis
- Definition
- Before you begin
- Key resources table
- Materials and equipment
- Step-by-step method details
- Expected outcomes
- Advantages
- Limitations
- Optimization and troubleshooting
- Problem
- Potential solution
- Safety considerations and standards
- Step-by-step method details
- Expected outcomes
- Advantages
- Limitations
- Optimization and troubleshooting
- Problem
- Potential solution
- Safety considerations and standards
- References
- Chapter 7 Genomic DNA extraction from the plant leaves using the CTAB method
- Definition
- Rationale
- Before you begin
- Key resources table
- Materials and equipment
- Step-by-step method details
- Expected outcomes
- Quantification and statistical analysis
- Advantages
- Limitations
- Optimization and troubleshooting
- Problem
- Potential solution.