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| LEADER |
00000cam a2200000 a 4500 |
| 001 |
SCIDIR_on1202052323 |
| 003 |
OCoLC |
| 005 |
20231120010515.0 |
| 006 |
m o d |
| 007 |
cr |n||||||||| |
| 008 |
201028s2020 mau o 000 0 eng d |
| 040 |
|
|
|a YDX
|b eng
|e pn
|c YDX
|d OPELS
|d EBLCP
|d CS1
|d OCLCF
|d OCLCO
|d CASUM
|d OCLCO
|d OCLCQ
|d OCLCO
|d SFB
|d OCLCQ
|d OCLCO
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| 019 |
|
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|a 1202228800
|a 1241946027
|
| 020 |
|
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|a 9780128206638
|q (electronic bk.)
|
| 020 |
|
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|a 0128206632
|q (electronic bk.)
|
| 020 |
|
|
|z 9780128206621
|
| 020 |
|
|
|z 0128206624
|
| 035 |
|
|
|a (OCoLC)1202052323
|z (OCoLC)1202228800
|z (OCoLC)1241946027
|
| 050 |
|
4 |
|a QH602
|
| 082 |
0 |
4 |
|a 571.6/55
|2 23
|
| 245 |
0 |
0 |
|a Extracellular vesicles /
|c edited by Sheila Spada, Lorenzo Galluzzi.
|
| 260 |
|
|
|a Cambridge, MA :
|b Academic Press,
|c 2020.
|
| 300 |
|
|
|a 1 online resource
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
|
|a Methods in enzymology ;
|v v. 645
|
| 505 |
0 |
|
|a Intro -- Extracellular Vesicles -- Copyright -- Contents -- Contributors -- Preface -- Extracellular vesicles: An exciting and rapidly expanding field of investigation -- Acknowledgments -- Disclosures -- References -- Chapter One: Genetic labeling of extracellular vesicles for studying biogenesis and uptake in living mammalian cells -- 1. Introduction -- 2. Equipment and materials -- 2.1. Equipment -- 2.2. Cells and culture medium -- 2.3. Reagents and chemicals -- 3. Protocol -- 3.1. Cell culture -- 3.2. Generation of transient and stable CD63-GFP/VSVG-GFP expressing cells
|
| 505 |
8 |
|
|a 3.3. Exosome preparation from conditioned medium -- 3.4. Cell and exosome imaging -- 3.5. Cellular uptake of exosomes by confocal microscopy and flow cytometry -- 4. Concluding remarks -- 5. Notes -- Acknowledgments -- References -- Chapter Two: Fluorescent labeling of extracellular vesicles -- 1. Introduction -- 1.1. Uptake studies -- 1.2. Biodistribution studies -- 1.3. Characterization studies -- 2. Fluorescent labeling -- 2.1. EV Labeling approaches -- 2.1.1. Surface marker labeling of EVs -- 2.1.2. Lipid membrane labeling of EVs -- 2.1.3. Luminal labeling of EVs
|
| 505 |
8 |
|
|a 2.2. Post-labeling clean-up -- 2.2.1. Differential ultracentrifugation -- 2.2.2. Density gradient centrifugation -- 2.2.3. Size exclusion chromatography -- 2.2.4. Filtration -- 3. Materials, equipment and reagents -- 4. Protocols -- 4.1. Sample labeling -- 4.2. Removing excess dye -- 4.3. Coverslip preparation -- 4.4. Fluorescent imaging -- 4.5. Size assessment using NTA -- 5. Pros and cons -- 5.1. Surface marker labeling -- 5.2. Lipid membrane labeling -- 5.3. Luminal labeling -- 6. Conclusion -- References
|
| 505 |
8 |
|
|a Chapter Three: Use of antibody arrays to probe exosome and extracellular vesicle mediated functional changes in cells -- 1. Introduction -- 1.1. Exosomes and extracellular vesicles -- 1.2. Antibody arrays -- 2. Methods -- 2.1. Cell culture -- 2.2. Isolation of exosome fraction -- 2.3. Labeling of exosome fraction -- 2.4. Treatment of cells -- 2.5. Antibody arrays -- 3. Notes -- 4. Concluding remarks -- References -- Chapter Four: Analysis of individual extracellular vesicles by imaging flow cytometry -- 1. Introduction -- 2. Single EV analyses -- 2.1. Equipment -- 2.2. Materials
|
| 505 |
8 |
|
|a 2.3. Protocol optimization -- 2.4. Instrument calibration -- 3. Protocol -- 3.1. Preparation of antibody solution -- 3.2. Sample preparation -- 3.3. Controls -- 3.4. Sample acquisition with the ISX -- 4. Data analysis -- 4.1. Defining of masks to analyze the raw image file data -- 4.2. Using the feature manager to determine single events -- 4.3. Data presentation and calculation of subset concentrations -- 5. Summary -- Acknowledgments -- References -- Chapter Five: Imaging intercellular interaction and extracellular vesicle exchange in a co-culture model of chronic lymph ... -- 1. Introduction
|
| 650 |
|
0 |
|a Liposomes.
|
| 650 |
|
0 |
|a Extracellular space.
|
| 650 |
1 |
2 |
|a Liposomes
|0 (DNLM)D008081
|
| 650 |
2 |
2 |
|a Extracellular Space
|0 (DNLM)D005110
|
| 650 |
|
6 |
|a Liposomes.
|0 (CaQQLa)201-0009732
|
| 650 |
|
6 |
|a Espace extracellulaire.
|0 (CaQQLa)201-0041943
|
| 650 |
|
7 |
|a Extracellular space
|2 fast
|0 (OCoLC)fst00918998
|
| 650 |
|
7 |
|a Liposomes
|2 fast
|0 (OCoLC)fst00999411
|
| 655 |
|
4 |
|a Internet Resources.
|
| 655 |
|
4 |
|a Index not Present.
|
| 700 |
1 |
|
|a Spada, Sheila.
|
| 700 |
1 |
|
|a Galluzzi, Lorenzo.
|
| 776 |
0 |
8 |
|i Ebook version :
|z 9780128206638
|
| 776 |
0 |
8 |
|i Print version:
|t Extracellular vesicles.
|d Cambridge, MA : Academic Press, 2020
|z 0128206624
|z 9780128206621
|w (OCoLC)1150979594
|
| 830 |
|
0 |
|a Methods in enzymology ;
|v v. 645.
|
| 856 |
4 |
0 |
|u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/645
|z Texto completo
|
