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PCR protocols : a guide to methods and applications /
"The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. Avoid contamination--with specific instructions on setting up your lab Avoid cumbersome molecular biological techniques Discover...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
[1990]
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Optimization of PCRs / Michael A. Innis and David H. Gelfand
- Amplification of genomic DNA / Randall K. Saiki
- Amplification of RNA / Ernest S. Kawasaki
- RACE : rapid amplification of cDNA ends / Michael A. Frohman
- Degenerate primers for DNA amplification / Teresa Compton
- cDNA cloning using degenerate primers / Cheng Chi Lee and C. Thomas Caskey
- PCR with 7-Deaza-2'-deoxyguanosine triphosphate / Michael A. Innis
- Competitive PCR for quantitation of mRNA / Gary Gilliland, Steven Perrin, and H. Franklin Bunn
- Quantitative PCR / Alice M. Wang and David F. Mark
- Production of single-stranded DNA by asymmetric PCR / Peter C. McCabe
- Cloning with PCR / Stephen J. Scharf
- Oligonucleotide ligation assay / Ulf Landegren, Robert Kaiser, and Leroy Hood.
- Nonisotopically labeled probes and primers / Corey Levenson and Chu-an Chang
- Incorporation of biotinylated dUTP / Y.-M. Dennis Lo, Wajahat Z. Mehal, and Kenneth A. Fleming
- Nonisotopic detection of PCR products / Rhea Helmuth
- Thermostable DNA polymerases / David H. Gelfand and Thomas J. White
- Procedures to minimize PCR-product carry-over / Shirley Kwok
- Sample preparation from blood, cells, and other fluids / Ernest S. Kawasaki
- Sample preparation from paraffin-embedded tissues / Deann K. Wright and M. Michele Manos
- Amplifying ancient DNA / Svante P�a�abo
- In vitro transcription of PCR templates / Michael J. Holland and Michael A. Innis
- Recombinant PCR / Russell Higuchi
- DNase I footprinting / Barbara Krummel
- Sequencing with Taq DNA polymerase / Mary Ann D. Brow.
- Direct sequencing with the aid of phage promoters / Steve S. Sommer [and others]
- Identifying DNA polymorphisms by denaturing gradient gel electrophoresis / Val C. Sheffield, David R. Cox, and Richard M. Meyers
- Amplification of flanking sequences by inverse PCR / Howard Ochman [and others]
- Detection of homologous recombinants / Michael A. Frohman and Gail R. Martin
- RNA processing : Apo-B / Lyn M. Powell
- A transcription-based amplification system / T.R. Gingeras [and others]
- Screening of [lambda] gt11 libraries / Kenneth D. Friedman [and others]
- HLA DNA typing / Henry A. Erlich and Teodorica L. Bugawan
- Multiplex PCR for the diagnosis of Duchenne muscular dystrophy / Jeffrey S. Chamberlain [and others]
- Isolation of DNA from fungal mycelia and single spores / Steven B. Lee and John W. Taylor
- Genetic prediction of hemophilia A / Scott C. Kogan and Jane Gitschier.
- Haplotype analysis from single sperm or diploid cells / Ulf Gyllensten
- Amplification of ribosomal RNA genes for molecular evolution studies / Mitchell L. Sogin
- Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics / T.J. White [and others]
- Detection of human T-cell lymphoma/leukemia viruses / Garth D. Ehrlich, Steven Greenberg, and Mark A. Abbott
- Detection of Human Immunodeficiency Virus / David E. Kellogg and Shirley Kwok
- Detection of hepatitis B virus / I. Baginski [and others]
- Detection and typing of genital human papillomaviruses / Yi Ting and M. Michele Manos
- Detection of human cytomegalovirus / Darryl Shibata.
- PCR amplification of enteroviruses / Harley A. Rotbart
- Novel viruses / David Mack, Oh-Sik Kwon, and Fred Faloona
- Analysis of Ras gene point mutations by PCR and oligonucleotide hybridization / John Lyons
- B-cell lymphoma : t(14;18) chromosome rearrangement / Marco Crescenzi
- Detecting bacterial pathogens in environmental water samples by using PCR and gene probes / Ronald M. Atlas and Asim K. Bej
- PCR in the diagnosis of retinoblastoma / Sang-Ho Park
- Determination of familial relationships / Cristi�an Orrego and Mary Claire King
- PCR in a teacup : a simple and inexpensive method for thermocycling PCRs / Robert Watson
- A low-cost air-driven cycling oven / Peter Denton and H. Reisner.
- Modification of a Histokinette for use as an automated PCR machine / N.C.P. Cross [and others]
- Organizing a laboratory for PCR work / Cristi�an Orrego
- Basic equipment and supplies / Roberta Madej and Stephen Scharf.