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PCR protocols : a guide to methods and applications /
"The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. Avoid contamination--with specific instructions on setting up your lab Avoid cumbersome molecular biological techniques Discover...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
[1990]
|
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 245 | 0 | 0 | |a PCR protocols : |b a guide to methods and applications / |c edited by Michael A. Innis [and others]. |
| 264 | 1 | |a San Diego : |b Academic Press, |c [1990] | |
| 264 | 4 | |c �1990 | |
| 300 | |a 1 online resource (xviii, 482 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | |a Optimization of PCRs / Michael A. Innis and David H. Gelfand -- Amplification of genomic DNA / Randall K. Saiki -- Amplification of RNA / Ernest S. Kawasaki -- RACE : rapid amplification of cDNA ends / Michael A. Frohman -- Degenerate primers for DNA amplification / Teresa Compton -- cDNA cloning using degenerate primers / Cheng Chi Lee and C. Thomas Caskey -- PCR with 7-Deaza-2'-deoxyguanosine triphosphate / Michael A. Innis -- Competitive PCR for quantitation of mRNA / Gary Gilliland, Steven Perrin, and H. Franklin Bunn -- Quantitative PCR / Alice M. Wang and David F. Mark -- Production of single-stranded DNA by asymmetric PCR / Peter C. McCabe -- Cloning with PCR / Stephen J. Scharf -- Oligonucleotide ligation assay / Ulf Landegren, Robert Kaiser, and Leroy Hood. | |
| 505 | 0 | |a Nonisotopically labeled probes and primers / Corey Levenson and Chu-an Chang -- Incorporation of biotinylated dUTP / Y.-M. Dennis Lo, Wajahat Z. Mehal, and Kenneth A. Fleming -- Nonisotopic detection of PCR products / Rhea Helmuth -- Thermostable DNA polymerases / David H. Gelfand and Thomas J. White -- Procedures to minimize PCR-product carry-over / Shirley Kwok -- Sample preparation from blood, cells, and other fluids / Ernest S. Kawasaki -- Sample preparation from paraffin-embedded tissues / Deann K. Wright and M. Michele Manos -- Amplifying ancient DNA / Svante P�a�abo -- In vitro transcription of PCR templates / Michael J. Holland and Michael A. Innis -- Recombinant PCR / Russell Higuchi -- DNase I footprinting / Barbara Krummel -- Sequencing with Taq DNA polymerase / Mary Ann D. Brow. | |
| 505 | 0 | |a Direct sequencing with the aid of phage promoters / Steve S. Sommer [and others] -- Identifying DNA polymorphisms by denaturing gradient gel electrophoresis / Val C. Sheffield, David R. Cox, and Richard M. Meyers -- Amplification of flanking sequences by inverse PCR / Howard Ochman [and others] -- Detection of homologous recombinants / Michael A. Frohman and Gail R. Martin -- RNA processing : Apo-B / Lyn M. Powell -- A transcription-based amplification system / T.R. Gingeras [and others] -- Screening of [lambda] gt11 libraries / Kenneth D. Friedman [and others] -- HLA DNA typing / Henry A. Erlich and Teodorica L. Bugawan -- Multiplex PCR for the diagnosis of Duchenne muscular dystrophy / Jeffrey S. Chamberlain [and others] -- Isolation of DNA from fungal mycelia and single spores / Steven B. Lee and John W. Taylor -- Genetic prediction of hemophilia A / Scott C. Kogan and Jane Gitschier. | |
| 505 | 0 | |a Haplotype analysis from single sperm or diploid cells / Ulf Gyllensten -- Amplification of ribosomal RNA genes for molecular evolution studies / Mitchell L. Sogin -- Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics / T.J. White [and others] -- Detection of human T-cell lymphoma/leukemia viruses / Garth D. Ehrlich, Steven Greenberg, and Mark A. Abbott -- Detection of Human Immunodeficiency Virus / David E. Kellogg and Shirley Kwok -- Detection of hepatitis B virus / I. Baginski [and others] -- Detection and typing of genital human papillomaviruses / Yi Ting and M. Michele Manos -- Detection of human cytomegalovirus / Darryl Shibata. | |
| 505 | 0 | |a PCR amplification of enteroviruses / Harley A. Rotbart -- Novel viruses / David Mack, Oh-Sik Kwon, and Fred Faloona -- Analysis of Ras gene point mutations by PCR and oligonucleotide hybridization / John Lyons -- B-cell lymphoma : t(14;18) chromosome rearrangement / Marco Crescenzi -- Detecting bacterial pathogens in environmental water samples by using PCR and gene probes / Ronald M. Atlas and Asim K. Bej -- PCR in the diagnosis of retinoblastoma / Sang-Ho Park -- Determination of familial relationships / Cristi�an Orrego and Mary Claire King -- PCR in a teacup : a simple and inexpensive method for thermocycling PCRs / Robert Watson -- A low-cost air-driven cycling oven / Peter Denton and H. Reisner. | |
| 505 | 0 | |a Modification of a Histokinette for use as an automated PCR machine / N.C.P. Cross [and others] -- Organizing a laboratory for PCR work / Cristi�an Orrego -- Basic equipment and supplies / Roberta Madej and Stephen Scharf. | |
| 506 | |3 Use copy |f Restrictions unspecified |2 star |5 MiAaHDL | ||
| 520 | |a "The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. Avoid contamination--with specific instructions on setting up your lab Avoid cumbersome molecular biological techniques Discover new applications." | ||
| 533 | |a Electronic reproduction. |b [Place of publication not identified] : |c HathiTrust Digital Library, |d 2010. |5 MiAaHDL | ||
| 538 | |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |u http://purl.oclc.org/DLF/benchrepro0212 |5 MiAaHDL | ||
| 583 | 1 | |a digitized |c 2010 |h HathiTrust Digital Library |l committed to preserve |2 pda |5 MiAaHDL | |
| 588 | 0 | |a Online resource; title from PDF title page (ScienceDirect, viewed October 10, 2023). | |
| 650 | 0 | |a Polymerase chain reaction. | |
| 650 | 0 | |a Gene amplification. | |
| 650 | 0 | |a DNA polymerases. | |
| 650 | 0 | |a RNA polymerases. | |
| 650 | 1 | |a Gene amplification. | |
| 650 | 2 | |a Genetic Engineering |x methods |0 (DNLM)D005818Q000379 | |
| 650 | 2 | |a Nucleic Acid Amplification Techniques |0 (DNLM)D021141 | |
| 650 | 2 | |a Polymerase Chain Reaction |0 (DNLM)D016133 | |
| 650 | 2 | |a Gene Amplification |0 (DNLM)D005784 | |
| 650 | 2 | |a DNA-Directed RNA Polymerases |0 (DNLM)D012321 | |
| 650 | 3 | |a Gene Amplification |x methods. | |
| 650 | 5 | |a RNA Polymerases. | |
| 650 | 6 | |a R�eaction en cha�ine de la polym�erase. |0 (CaQQLa)201-0086483 | |
| 650 | 6 | |a Amplification g�enique. |0 (CaQQLa)201-0124192 | |
| 650 | 6 | |a ADN polym�erases. |0 (CaQQLa)201-0086482 | |
| 650 | 6 | |a ARN polym�erases. |0 (CaQQLa)201-0024709 | |
| 650 | 6 | |a Amplification des acides nucl�eiques |x Technique. |0 (CaQQLa)000326951 | |
| 650 | 7 | |a 35.75 proteins, nucleic acids. |0 (NL-LeOCL)087981696 |2 bcl | |
| 650 | 7 | |a DNA Polymerase. |2 cabt | |
| 650 | 7 | |a RNA Polymerase. |2 cabt | |
| 650 | 7 | |a Polymerase Chain Reaction. |2 cabt | |
| 650 | 7 | |a RNA polymerases |2 fast |0 (OCoLC)fst01086256 | |
| 650 | 7 | |a DNA polymerases |2 fast |0 (OCoLC)fst00886595 | |
| 650 | 7 | |a Gene amplification |2 fast |0 (OCoLC)fst00939610 | |
| 650 | 7 | |a Polymerase chain reaction |2 fast |0 (OCoLC)fst01070553 | |
| 650 | 7 | |a G�en�etique |x Ing�enierie |x M�ethodes. |2 fmesh | |
| 650 | 7 | |a Amplification g�en�etique |x M�ethodes. |2 fmesh | |
| 650 | 7 | |a R�eaction de polym�erisation en cha�ine. |2 fmesh | |
| 650 | 7 | |a G�enie g�en�etique |x M�ethodes. |2 fmesh | |
| 650 | 7 | |a DNA. |2 fss | |
| 650 | 7 | |a Genetic engineering. |2 fss | |
| 650 | 7 | |a Polymerase chain reaction. |2 fss | |
| 650 | 7 | |a Genamplifikation |2 gnd |0 (DE-588)4333686-3 | |
| 650 | 7 | |a Gentechnologie |2 gnd |0 (DE-588)4071722-7 | |
| 650 | 7 | |a Polymerase-Kettenreaktion |2 gnd |0 (DE-588)4256726-9 | |
| 650 | 7 | |a Polymerasen |2 gnd |0 (DE-588)4175227-2 | |
| 650 | 1 | 7 | |a DNA. |2 gtt |
| 650 | 1 | 7 | |a Polymerase kettingreactie. |2 gtt |
| 650 | 1 | 7 | |a Genetische manipulatie. |2 gtt |
| 650 | 1 | 7 | |a RNA-polymerasen. |2 gtt |
| 650 | 7 | |a ADN polym�erases. |2 ram | |
| 650 | 7 | |a ARN polym�erases. |2 ram | |
| 650 | 7 | |a R�eaction en cha�ine de la polym�erase. |2 ram | |
| 650 | 7 | |a Amplification g�enique. |2 ram | |
| 653 | |a Cadena de reacci�on de la polimerasa. | ||
| 653 | |a ADN polimerasa. | ||
| 653 | |a ARN polimerasa. | ||
| 653 | 0 | 0 | |a genetische modificatie |
| 653 | 0 | 0 | |a genetic engineering |
| 653 | 0 | 0 | |a recombinant dna |
| 653 | 0 | 0 | |a nucleic acids |
| 653 | 0 | 0 | |a handleidingen |
| 653 | 0 | 0 | |a guide books |
| 653 | 0 | 0 | |a methodologie |
| 653 | 0 | 0 | |a methodology |
| 653 | 0 | 0 | |a technieken |
| 653 | 0 | 0 | |a techniques |
| 653 | 0 | 0 | |a moleculaire biologie |
| 653 | 0 | 0 | |a molecular biology |
| 653 | 0 | 0 | |a polymerase-kettingreactie |
| 653 | 0 | 0 | |a polymerase chain reaction |
| 653 | 0 | 0 | |a protocollen |
| 653 | 0 | 0 | |a protocols |
| 653 | 1 | 0 | |a Nucleic Acids |
| 653 | 2 | 0 | |a Biological Techniques |
| 653 | 2 | 0 | |a Biologische technieken |
| 655 | 2 | |a Laboratory Manual |0 (DNLM)D020484 | |
| 655 | 7 | |a Laboratory manuals |2 fast |0 (OCoLC)fst01920776 | |
| 655 | 7 | |a Laboratory manuals. |2 lcgft | |
| 655 | 7 | |a Manuels de laboratoire. |2 rvmgf |0 (CaQQLa)RVMGF-000001214 | |
| 700 | 1 | |a Innis, Michael A., |e ditor. | |
| 776 | 0 | 8 | |i Online version: |t PCR protocols. |d San Diego : Academic Press, �1990 |w (OCoLC)609123717 |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780123721808 |z Texto completo |