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Kidney biomarkers : clinical aspects and laboratory determination /
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam :
Academic Press,
2020.
|
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Further reading
- Chapter 2: Utility of the ``omics�� in kidney disease: Methods of analysis, sampling considerations, and technical approa ...
- Introduction
- Statistical methods in analysis and interpretation of biomarkers in kidney disease
- Metrics for prediction performance (diagnostic potential)
- Quantification of prediction performance
- True positive rate and false positive rate
- Receiver-operating characteristic curve
- Summary indices
- Area under the ROC curve (AUC)
- Optimal prediction threshold
- Partial area under the curve
- Limitations of statistical metrics used for prediction performance
- Metrics to evaluating incremental diagnostic value
- Incremental value: How to evaluate?
- Improvement in discrimination
- Improvement in reclassification
- Net reclassification improvement
- Categorical net reclassification improvement
- Weighted net reclassification improvement
- Category-free net reclassification improvement
- Integrated discrimination improvement
- Decision and clinical use of analytic measures
- Recommendations for sample collection, storage, and preparation
- Tissue samples
- Biofluids
- Nontargeted versus targeted analysis
- Proteomics and metabolomics in nephrology
- Potential impact of proteomics and metabolomics in renal diseases
- Proteomics and the kidney
- The proteomics sample
- Urine
- Kidney tissue and cell culture
- Techniques used in proteome analysis
- Proteomics methods based on mass spectrometry
- First step: Protein separation
- Gel-based proteome analysis
- Gel-free proteome analysis
- Liquid chromatography (LC)
- Capillary electrophoresis
- Second step: Protein identification
- Two-dimensional gel electrophoresis coupled with mass spectrometry (2DE-MS)
- Liquid chromatography coupled to mass spectrometry (LC-MS/MS)
- Capillary electrophoresis coupled with mass spectrometry (CE-MS)
- Matrix-assisted laser desorption/ionization-time of flight coupled with mass spectrometry (MALDI-TOF/MS)
- Solid-phase chromatography coupled with mass spectrometry (SELDI-MS)
- Differential protein profiling
- Mass spectrometry
- (a) Peptide mass fingerprinting
- (b) Electrospray ionization
- (c) Peptide sequencing using tandem mass spectrometers