Retinoid signaling pathways /

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Pohl, Ehmke (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Cambridge, MA : Academic Press, 2020.
Colección:Methods in enzymology ; v. 637.
Temas:
Retinoids.
Cellular signal transduction.
Retinoids
Signal Transduction
R�etino�ides.
Transduction du signal cellulaire.
Cellular signal transduction
Internet Resources.
Index not Present.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Intro
  • Retinoid Signaling Pathways
  • Copyright
  • Contents
  • Contributors
  • Preface
  • Chapter One: Vitamin A and its natural derivatives
  • 1. Vitamin A: Molecules and biochemical properties
  • 1.1. Vitamin A brief history and nomenclature
  • 1.2. Natural derivatives of retinol
  • 1.3. Lipid properties of retinoids
  • 1.4. Physiological concentrations of natural retinoids
  • 1.5. Retinoid signaling: Delivery of retinol to tissues and cells
  • 2. Retinoid signaling in vision
  • 3. Retinoid signaling in growth and development
  • 4. Future work
  • References
  • Chapter Two: Post-natal all-trans-retinoic acid biosynthesis
  • 1. Introduction
  • 2. ATRA and hormesis
  • 3. Retinol dehydrogenases
  • 3.1. Background
  • 3.2. Functions of mRdh1/hRdh16
  • 3.3. Rdh10 functions
  • 3.3.1. Post-natal, non-ocular Rdh10 functions
  • 3.3.2. Rdh10 and cancer
  • 3.4. Dhrs9 contributions to ATRA biosynthesis
  • 3.4.1. Dhrs9 in hair and skin
  • 3.4.2. Dhrs9 and tumor progression
  • 3.4.2.1. Dhrs9 and immunity
  • 3.5. RdhE2 and E2S (retinol dehydrogenases epidermal 2 and 2 similar)
  • 3.6. Other Rdh
  • 3.7. A cautionary note
  • 4. Retinal reductases (Rrd) and retinoid metabolism
  • 4.1. The reductase Dhrs3 (mouse and human retSDR1, hSdr16C1, hRdh17)
  • 4.1.1. Dhrs3 and cancer
  • 4.2. An uncertain contribution of Rdh13 (Pan2)
  • 4.3. The reductase Dhrs4 (mouse Rrd
  • human 2,4-dienoyl-CoA reductase)
  • 4.4. The reductase Rdh11 (mouse and human RalR1, Psdr1 scald and human Sdr7C1)
  • 5. Regulation of ATRA tissue concentrations
  • 6. The multiplicity of enzymes in the retinoid metabolon
  • 7. Alcohol dehydrogenases
  • 8. Retinal dehydrogenases
  • 8.1. Raldh and disease
  • References
  • 4.5. Chromatography
  • 4.6. Kinetic studies
  • 4.7. Analysis and statistics
  • 4.8. Non-genomic BP functions
  • 5. Summary
  • References
  • Chapter Four: Generation and isolation of recombinant retinoid oxidoreductase complex
  • 1. Introduction
  • 2. The short-chain dehydrogenase/reductase 16C (SDR16C) family of enzymes
  • 3. The retinoid oxidoreductase complex (ROC)
  • 4. The baculovirus expression system
  • 5. Calcium phosphate precipitation
  • 6. Protocol
  • 6.1. Equipment
  • 6.2. Reagents
  • 6.3. Buffer preparation
  • 6.3.1. Recombinant baculovirus generation buffers
  • 6.3.2. Subcellular fractionation buffers
  • Chapter Three: Retinoid metabolism and functions mediated by retinoid binding-proteins
  • 1. Nature of the BP
  • 1.1. Crbp2 (encoded by rbp2)
  • 1.2. Crbp1 (encoded by rbp1)
  • 2. BP contributions to retinoid metabolism
  • 2.1. Crbp contributions to retinol and retinal metabolism
  • 2.2. Crabp contributions to ATRA catabolism
  • 2.3. Fabp5 and ATRA function
  • 3. Experimental methods
  • 3.1. Definition
  • 3.2. Rationale
  • 3.3. Materials, equipment and reagents
  • 4. Protocols
  • 4.1. BP preparation
  • 4.2. Retinoid purification
  • 4.3. UV analyses
  • 4.4. Tissue, cell and homogenate extraction.

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