Advances in enzyme catalysis and technologies /

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Singh, Sudhir P.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam : Elsevier, 2020.
Colección:Biomass, biofuels, biochemicals.
Temas:
Enzymes > Biotechnology.
Enzymes > Biotechnologie.
Enzymes > Biotechnology
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Front Cover
  • Biomass, Biofuels, Biochemicals
  • Copyright Page
  • Contents
  • List of contributors
  • Preface
  • 1 Fundamental aspects of enzymes
  • 1 An introduction to enzyme structure dynamics and enzyme catalysis
  • 1.1 Introduction
  • 1.2 Why do some proteins work as enzyme?
  • 1.2.1 Role of the active site in enzyme functioning
  • 1.2.2 Cofactor, a necessity of enzyme
  • 1.3 What is enzyme catalysis?
  • 1.3.1 Basics of enzyme catalysis
  • 1.3.2 Historical overview of enzyme catalysis theory
  • 1.3.3 Mechanistic view of enzyme catalysis
  • 1.4 Structural dynamics of enzymes
  • 1.5 Ribozymes as a nonprotein catalyst
  • 1.6 Conclusions and perspective
  • References
  • 2 Classification of enzymes and catalytic properties
  • 2.1 Introduction
  • 2.1.1 Enzyme nomenclature and classification
  • 2.1.2 Enzyme databases
  • 2.1.3 Catalytic properties
  • 2.2 Enzymes classes and properties
  • 2.2.1 Oxidoreductases
  • 2.2.2 Transferases
  • 2.2.3 Hydrolases
  • 2.2.3.1 Amylases
  • 2.2.3.2 Cellulases
  • 2.2.3.3 Xylanases
  • 2.2.3.4 Lipases
  • 2.2.3.5 Proteases
  • 2.2.4 Lyases
  • 2.2.5 Isomerases
  • 2.2.6 Ligases
  • 2.2.7 Translocases
  • 2.3 Conclusions and perspectives
  • References
  • Further reading
  • 3 Enzymes and their production strategies
  • 3.1 Introduction
  • 3.2 Enzymes and their classifications
  • 3.3 Enzyme production
  • 3.3.1 Microbial enzyme production
  • 3.3.1.1 Fermentation
  • 3.3.1.2 Recovery
  • 3.3.1.3 Purification
  • 3.3.1.4 Formulation
  • 3.3.2 Enzyme production from plants and animals
  • 3.4 Applications
  • 3.5 Conclusions and perspectives
  • Acknowledgments
  • References
  • 4 Robust enzymes designing for efficient biocatalysis
  • 4.1 Introduction
  • 4.2 Biocatalysis engineering-a driving force
  • 4.3 Recent advances in protein engineering
  • 4.3.1 Enzyme immobilization-a drive toward optimum performance
  • 4.3.2 Substrate engineering-a toolkit to harness the enzymatic promiscuity
  • 4.3.3 Structure-assisted protein engineering
  • 4.3.4 Advanced computational modeling
  • 4.3.5 Protein engineering via directed evolution and rational design
  • 4.4 Conclusions and perspectives
  • 4.5 Acknowledgments
  • 4.6 Conflict of interest
  • References
  • 2 Enzyme engineering for efficient biocatalytic reactions
  • 5 Enzyme engineering strategies to confer thermostability
  • 5.1 Introduction
  • 5.2 Enzyme engineering strategies for thermostabilization
  • 5.2.1 Directed evolution
  • 5.2.2 Rational/semi-rational design
  • 5.2.2.1 Sequence-based engineering
  • 5.2.2.1.1 Comparing sequences with higher thermostability
  • 5.2.2.1.2 Consensus analysis
  • 5.2.2.1.3 Ancestral sequence reconstruction
  • 5.2.2.2 Structure-based engineering
  • 5.2.2.2.1 Beta-factor analysis
  • 5.2.2.2.2 Molecular dynamic simulations
  • 5.2.2.2.3 FoldX and Rosetta_ddg
  • 5.2.2.2.4 Structure-guided sequence-based engineering
  • 5.2.3 De novo design
  • 5.2.4 Comprehensive computational approaches

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