Advances in biomembranes and lipid self-assembly. Volume 31 /

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Igli�c, Ale�s, Rappolt, Michael, Garc�ia-S�aez, Ana J.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: [Place of publication not identified] : Academic Press, 2020.
Colección:Issn Ser.
Temas:
Bilayer lipid membranes > Biotechnology.
Liposomes.
Liposomes
Bilayer lipid membranes > Biotechnology
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Front Cover
  • Advances in Biomembranes and Lipid Self-Assembly
  • Editorial Board
  • Advances in Biomembranes and Lipid Self-Assembly
  • Copyright
  • Contents
  • Contributors
  • Preface
  • One
  • Physico-chemical characterizations of lipid membranes in presence of cholesterol
  • 1. Introduction
  • 2. Materials
  • 2.1 Sample preparation for SEM
  • 2.2 Preparation of giant unilamellar vesicles
  • 2.3 Sample preparation for DSC
  • 2.4 Sample preparation for FTIR
  • 3. Methods
  • 3.1 Scanning electron microscopy (SEM)
  • 3.2 Thermally induced shape fluctuation analysis (TISFA)
  • 3.3 Differential scanning calorimetry (DSC)
  • 3.4 Fourier-Transform Infrared (FTIR) and Raman spectroscopy
  • 4. Results and discussion
  • 4.1 Scanning electron microscopy
  • 4.2 Bending elasticity modulus
  • 4.3 Phase behavior: phase transition temperature and enthalpy
  • 4.4 Fourier-transform infrared and Raman spectroscopy
  • 4.4.1 Hydrogen bonds detection in the interface region of SOPC. Interaction of cholesterol with the carbonyl CO group
  • 4.4.2 Influence of cholesterol interaction on the choline group
  • 4.4.3 Influence of cholesterol on intra- and interchain conformational ordering
  • 4.4.4 Correlation of FTIR and Raman spectra, and DSC for cholesterol induced structural conformation of the phosphatidylcholine h ...
  • 5. Conclusions
  • Acknowledgments
  • References
  • Two
  • Susceptibility of biomembrane structure towards amphiphiles, ionic liquids, and deep eutectic solvents
  • 1. Introduction
  • 2. When biologically active amphiphiles meet biomembranes
  • 2.1 Structural perturbating effects of DMSO on biomembranes
  • 2.2 Biomembranes under the alcoholic influence of ethanol
  • 2.3 How inhalant acetone influences biomembranes
  • 3. Interactions of ionic liquids with biomembranes and their adverse effects
  • 3.1 Emergence of environmentally benign bio-ILs and their effects on biomembranes
  • 4. Effect of deep eutectic solvents on biomembranes and lipid self-assembly
  • 5. Concluding remarks
  • Acknowledgments
  • References
  • Three
  • Quantitative single-molecule imaging of protein assembly in membranes
  • 1. Introduction
  • 2. General considerations about quantitative single molecule fluorescence image acquisition
  • 3. Quantitative investigation of protein assembly in model membranes
  • 3.1 Model membranes for a bottom-up approach to mimic biological membranes
  • 3.1.1 Lipid vesicles (liposomes)
  • 3.1.2 Supported lipid bilayers
  • 3.1.3 Membrane nanodiscs
  • 3.2 Fluorescent labeling of recombinant proteins
  • 3.2.1 Fluorescent labels and their photophysical properties
  • 3.2.2 Strategies for protein labeling with organic fluorophores
  • 3.3 Single-molecule imaging by TIRF microscopy
  • 3.4 Stoichiometry analysis of protein complexes in model membranes
  • 3.4.1 Photobleaching step counting method
  • 3.4.2 Single-molecule brightness analysis method

Ejemplares similares