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200401s2020 mau o 000 0 eng d |
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|a 1148234363
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|a 9780128201732
|q (electronic bk.)
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|a 0128201738
|q (electronic bk.)
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|z 9780128201725
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|z (OCoLC)1148234363
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|a Cell-derived matrices.
|p Part A
|h [electronic resource] /
|c edited by David Caballero, Subhas C. Kundu, Rui Lu�is Reis.
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| 260 |
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|a Cambridge :
|b Academic Press,
|c 2020.
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| 300 |
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|a 1 online resource
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|a text
|b txt
|2 rdacontent
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|a computer
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|2 rdamedia
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|a online resource
|b cr
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| 490 |
1 |
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|a Methods in cell biology ;
|v v. 156
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| 505 |
0 |
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|a Front Cover -- Cell-Derived Matrices -- Part A -- Copyright -- Contents -- Contributors -- Preface -- Section I: Decellularization and characterization methods -- Chapter 1: A step-by-step protocol for generating human fibroblast cell-derived completely biological extracellular matrix ... -- 1. Introduction -- 2. Materials and methods -- 2.1. PDMS substrate fabrication -- 2.2. Collagen grafting on PDMS substrates -- 2.3. Human dermal fibroblast sheet culture and decellularization procedure -- 3. Evaluation of hDF derived ECM scaffold -- 3.1. Immunofluorescence imaging
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| 505 |
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|a 3.2. Field emission scanning electron micrography -- 4. Discussion -- 5. Conclusion -- Acknowledgments -- References -- Chapter 2: Preparation of osteogenic matrices from cultured cells -- 1. Introduction -- 2. Osteogenic tissue culture and relevant assays -- 2.1. Human MSC expansion and osteogenic differentiation -- 2.1.1. Materials -- 2.1.2. Procedure -- 2.2. Assays of OPG secretion by enzyme linked immunosorbent assay (ELISA) -- 2.2.1. Materials -- 2.2.2. Procedure -- 2.3. Assays of ALP activity -- 2.3.1. Materials -- 2.3.2. Procedure -- 2.4. Mineralization assays -- 2.4.1. Materials
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|a 2.4.2. Procedure -- 2.5. Quantitative real-time RT-PCR (qRT-PCR) assays of biomarker transcription -- 3. Purification and characterization of OECM -- 3.1. Purification of matrix -- 3.1.1. Materials -- 3.1.2. Procedure -- 3.2. Assays of protein and calcium -- 3.2.1. Materials -- 3.2.2. Procedures -- 3.3. Morphology of OECM -- 3.4. Composition of OECM -- 3.4.1. Materials -- 3.4.2. Procedure -- 4. Coating of OECM onto growth surfaces -- 4.1. Solubilization and coating of OECM onto tissue culture plastic -- 4.1.1. Materials -- 4.1.2. Procedure
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|a 4.2. In situ coating of OECM by culture and decellularization -- 4.2.1. Materials -- 4.2.2. Procedure -- 5. Discussion, future prospects -- Acknowledgments -- References -- Chapter 3: Decellularized hASCs-derived matrices as biomaterials for 3D in vitro approaches -- 1. Introduction -- 2. Materials and methods -- 2.1. Reagents, equipment and material -- 2.2. CELL-derived ECM production -- 2.3. CELL-derived ECM production -- 3. Evaluation of effectiveness of the decellularization -- 3.1. Reagents, equipment and material -- 3.2. DNA quantification -- 3.3. Visualization of nuclear material
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|a 3.4. Qualitative evaluation of collagen -- 4. Discussion -- 5. Conclusions -- Acknowledgments -- References -- Chapter 4: Characterization of the elastic properties of extracellular matrix models by atomic force microscopy -- 1. Introduction -- 2. Material, equipments and reagents -- 2.1. Equipment -- 2.1.1. AFM -- 2.1.2. Optical microscope -- 2.1.3. Specific equipment for sample preparation -- 2.2. Material and reagents -- 2.2.1. Measurement liquid buffer -- 2.2.2. AFM cantilever-tip -- 3. Protocol to measure the mechanical properties of ECM models
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| 650 |
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0 |
|a Extracellular matrix.
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| 650 |
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2 |
|a Extracellular Matrix
|0 (DNLM)D005109
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| 650 |
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6 |
|a Substance fondamentale (Histologie)
|0 (CaQQLa)201-0129525
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| 650 |
|
7 |
|a Extracellular matrix
|2 fast
|0 (OCoLC)fst00918995
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| 700 |
1 |
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|a Caballero, David.
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| 700 |
1 |
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|a Kundu, S. C.
|q (Subhas Chandra)
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| 700 |
1 |
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|a Reis, Rui L.
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| 776 |
0 |
8 |
|i Print version:
|z 012820172X
|z 9780128201725
|w (OCoLC)1119625472
|
| 830 |
|
0 |
|a Methods in cell biology ;
|v v. 156.
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| 856 |
4 |
0 |
|u https://sciencedirect.uam.elogim.com/science/bookseries/0091679X/156
|z Texto completo
|
