Neutron crystallography in structural biology /

Detalles Bibliográficos
Clasificación:Libro Electrónico
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Cambridge : Academic Press, 2020.
Colección:Methods in enzymology ; v. 634.
Temas:
Crystallography.
Enzymology.
Crystallization.
Enzymes.
Crystallography
Enzymes
Molecular Biology > methods
Crystallization
Cristallographie.
Enzymologie.
Cristallisation.
crystallization.
enzyme.
Enzymology
Internet Resources.
Index not Present.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Intro
  • Neutron Crystallography in Structural Biology
  • Copyright
  • Contents
  • Contributors
  • Preface
  • Chapter One: Fundamentals of neutron crystallography in structural biology
  • 1. Introduction
  • 2. Basics of neutrons as a diffraction probe
  • 2.1. Neutrons as waves
  • 2.2. Scattering signatures of the elements and their isotopes
  • 2.3. Neutron energies and phonon interactions
  • 2.4. Neutrons: Their spin and their magnetic moment
  • 3. Types of neutron sources
  • 4. Neutron macromolecular crystallography instrument types
  • 5. Sample preparation
  • 6. Planning an experiment with neutrons
  • 7. Measuring your diffraction data
  • 8. Refining the molecular model against neutron data
  • 9. Validating your model
  • 10. Data reuse
  • 11. Concluding remarks
  • Acknowledgments
  • References
  • Further reading
  • Chapter Two: Large crystal growth for neutron protein crystallography
  • 1. Introduction
  • 2. Crystallization conditions, nucleation, and growth
  • 3. Protein solubility and the phase diagram
  • 4. Effect of temperature on protein crystallization
  • 5. Seeding
  • 5.1. Large volume, sitting drop vapor diffusion
  • 5.1.1. Carbonic anhydrase II (CA II)
  • 5.1.2. Human carbonic anhydrase IX surface variant (CA IXSV)
  • 5.2. Batch
  • 5.2.1. Urate oxidase (UO)
  • 5.3. Dialysis
  • 6. Conclusions
  • Acknowledgments
  • References
  • Chapter Three: Prospects for membrane protein crystals in NMX
  • 1. Introduction
  • 2. Microdialysis crystallization
  • 3. Capillary counter-diffusion crystallization
  • 4. Crystallization of SERCA by microdialysis and capillary counterdiffusion
  • 4.1. Equipment
  • 4.2. Chemicals
  • 4.3. Protocol
  • 4.3.1. Preparation of solubilized SERCA Protein (Ca2E1-AMPPCP form)
  • 4.3.2. Crystallization of the SERCA Ca2E1-AMPPCP form by Microdialysis
  • 4.3.3. Crystallization of the SERCA Ca2E1-ADP:AlF4 form by Capillary Counterdiffusion
  • 4.3.4. Alternative method for crystallizing SERCA Ca2E1-ADP:AlF4 form by Capillary Counterdiffusion (with gelled batch so ...
  • 5. Summary
  • Acknowledgment
  • Funding information
  • References
  • Chapter Four: IMAGINE: The neutron protein crystallography beamline at the high flux isotope reactor
  • 1. Introduction
  • 2. Beamline overview
  • 2.1. Neutron source
  • 2.2. Beamline optics
  • 2.3. Diffractometer
  • 2.4. Data collection and reduction
  • 2.5. Structure refinement
  • 3. Sample preparation and ancillary facilities
  • 4. Facility access
  • 5. Highlights
  • 5.1. Neurospora Crassa lytic polysaccharide monooxygenase 9D
  • 5.2. H-RAS GTPase
  • 5.3. Neutron protein crystallography at cryogenic temperature
  • 6. Future development
  • 7. Summary
  • Acknowledgments
  • References
  • Chapter Five: The macromolecular neutron diffractometer at the spallation neutron source
  • 1. Introduction
  • 2. Auto reduction of diffraction data
  • 3. Early science highlights
  • 4. Human manganese superoxide dismutase

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