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Neutron crystallography in structural biology /

Detalles Bibliográficos
Clasificación:Libro Electrónico
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Cambridge : Academic Press, 2020.
Colección:Methods in enzymology ; v. 634.
Temas:
Acceso en línea:Texto completo

MARC

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020 |z 9780128192146 
020 |z 0128192143 
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050 4 |a QD905.2 
082 0 4 |a 548  |2 23 
245 0 0 |a Neutron crystallography in structural biology /  |c edited by Peter C.E. Moody. 
260 |a Cambridge :  |b Academic Press,  |c 2020. 
300 |a 1 online resource (xvii, 389 pages) :  |b illustrations 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in enzymology ;  |v volume 634 
505 0 |a Intro -- Neutron Crystallography in Structural Biology -- Copyright -- Contents -- Contributors -- Preface -- Chapter One: Fundamentals of neutron crystallography in structural biology -- 1. Introduction -- 2. Basics of neutrons as a diffraction probe -- 2.1. Neutrons as waves -- 2.2. Scattering signatures of the elements and their isotopes -- 2.3. Neutron energies and phonon interactions -- 2.4. Neutrons: Their spin and their magnetic moment -- 3. Types of neutron sources -- 4. Neutron macromolecular crystallography instrument types -- 5. Sample preparation 
505 8 |a 6. Planning an experiment with neutrons -- 7. Measuring your diffraction data -- 8. Refining the molecular model against neutron data -- 9. Validating your model -- 10. Data reuse -- 11. Concluding remarks -- Acknowledgments -- References -- Further reading -- Chapter Two: Large crystal growth for neutron protein crystallography -- 1. Introduction -- 2. Crystallization conditions, nucleation, and growth -- 3. Protein solubility and the phase diagram -- 4. Effect of temperature on protein crystallization -- 5. Seeding -- 5.1. Large volume, sitting drop vapor diffusion 
505 8 |a 5.1.1. Carbonic anhydrase II (CA II) -- 5.1.2. Human carbonic anhydrase IX surface variant (CA IXSV) -- 5.2. Batch -- 5.2.1. Urate oxidase (UO) -- 5.3. Dialysis -- 6. Conclusions -- Acknowledgments -- References -- Chapter Three: Prospects for membrane protein crystals in NMX -- 1. Introduction -- 2. Microdialysis crystallization -- 3. Capillary counter-diffusion crystallization -- 4. Crystallization of SERCA by microdialysis and capillary counterdiffusion -- 4.1. Equipment -- 4.2. Chemicals -- 4.3. Protocol -- 4.3.1. Preparation of solubilized SERCA Protein (Ca2E1-AMPPCP form) 
505 8 |a 4.3.2. Crystallization of the SERCA Ca2E1-AMPPCP form by Microdialysis -- 4.3.3. Crystallization of the SERCA Ca2E1-ADP:AlF4 form by Capillary Counterdiffusion -- 4.3.4. Alternative method for crystallizing SERCA Ca2E1-ADP:AlF4 form by Capillary Counterdiffusion (with gelled batch so ... -- 5. Summary -- Acknowledgment -- Funding information -- References -- Chapter Four: IMAGINE: The neutron protein crystallography beamline at the high flux isotope reactor -- 1. Introduction -- 2. Beamline overview -- 2.1. Neutron source -- 2.2. Beamline optics -- 2.3. Diffractometer 
505 8 |a 2.4. Data collection and reduction -- 2.5. Structure refinement -- 3. Sample preparation and ancillary facilities -- 4. Facility access -- 5. Highlights -- 5.1. Neurospora Crassa lytic polysaccharide monooxygenase 9D -- 5.2. H-RAS GTPase -- 5.3. Neutron protein crystallography at cryogenic temperature -- 6. Future development -- 7. Summary -- Acknowledgments -- References -- Chapter Five: The macromolecular neutron diffractometer at the spallation neutron source -- 1. Introduction -- 2. Auto reduction of diffraction data -- 3. Early science highlights -- 4. Human manganese superoxide dismutase 
650 0 |a Crystallography. 
650 0 |a Enzymology. 
650 0 |a Crystallization. 
650 0 |a Enzymes. 
650 1 2 |a Crystallography  |0 (DNLM)D003461 
650 1 2 |a Enzymes  |0 (DNLM)D004798 
650 1 2 |a Molecular Biology  |x methods  |0 (DNLM)D008967Q000379 
650 2 2 |a Crystallization  |0 (DNLM)D003460 
650 6 |a Cristallographie.  |0 (CaQQLa)201-0014886 
650 6 |a Enzymologie.  |0 (CaQQLa)201-0269407 
650 6 |a Cristallisation.  |0 (CaQQLa)201-0018431 
650 6 |a Enzymes.  |0 (CaQQLa)201-0002557 
650 7 |a crystallization.  |2 aat  |0 (CStmoGRI)aat300214731 
650 7 |a enzyme.  |2 aat  |0 (CStmoGRI)aat300212647 
650 7 |a Enzymes  |2 fast  |0 (OCoLC)fst00913605 
650 7 |a Crystallization  |2 fast  |0 (OCoLC)fst00884644 
650 7 |a Crystallography  |2 fast  |0 (OCoLC)fst00884652 
650 7 |a Enzymology  |2 fast  |0 (OCoLC)fst00913635 
655 4 |a Internet Resources. 
655 4 |a Index not Present. 
776 0 8 |i Print version:  |z 0128192143  |z 9780128192146  |w (OCoLC)1109811985 
830 0 |a Methods in enzymology ;  |v v. 634. 
856 4 0 |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/634  |z Texto completo