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SCIDIR_on1141959420 |
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OCoLC |
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20231120010438.0 |
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200226s2020 maua o 000 0 eng d |
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|a YDX
|b eng
|e pn
|c YDX
|d UIU
|d EBLCP
|d OPELS
|d UKAHL
|d SCB
|d UKMGB
|d BRX
|d OCLCF
|d CASUM
|d OCLCO
|d OCLCQ
|d OCLCO
|d COM
|d OCL
|d OCLCQ
|d OCLCO
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|a GBC009298
|2 bnb
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|a 019682746
|2 Uk
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|a 1142412502
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|a 9780128192153
|q (electronic bk.)
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|a 0128192151
|q (electronic bk.)
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|z 9780128192146
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|z 0128192143
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035 |
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|a (OCoLC)1141959420
|z (OCoLC)1142412502
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050 |
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4 |
|a QD905.2
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082 |
0 |
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|a 548
|2 23
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|a Neutron crystallography in structural biology /
|c edited by Peter C.E. Moody.
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260 |
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|a Cambridge :
|b Academic Press,
|c 2020.
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300 |
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|a 1 online resource (xvii, 389 pages) :
|b illustrations
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336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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1 |
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|a Methods in enzymology ;
|v volume 634
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505 |
0 |
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|a Intro -- Neutron Crystallography in Structural Biology -- Copyright -- Contents -- Contributors -- Preface -- Chapter One: Fundamentals of neutron crystallography in structural biology -- 1. Introduction -- 2. Basics of neutrons as a diffraction probe -- 2.1. Neutrons as waves -- 2.2. Scattering signatures of the elements and their isotopes -- 2.3. Neutron energies and phonon interactions -- 2.4. Neutrons: Their spin and their magnetic moment -- 3. Types of neutron sources -- 4. Neutron macromolecular crystallography instrument types -- 5. Sample preparation
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505 |
8 |
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|a 6. Planning an experiment with neutrons -- 7. Measuring your diffraction data -- 8. Refining the molecular model against neutron data -- 9. Validating your model -- 10. Data reuse -- 11. Concluding remarks -- Acknowledgments -- References -- Further reading -- Chapter Two: Large crystal growth for neutron protein crystallography -- 1. Introduction -- 2. Crystallization conditions, nucleation, and growth -- 3. Protein solubility and the phase diagram -- 4. Effect of temperature on protein crystallization -- 5. Seeding -- 5.1. Large volume, sitting drop vapor diffusion
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505 |
8 |
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|a 5.1.1. Carbonic anhydrase II (CA II) -- 5.1.2. Human carbonic anhydrase IX surface variant (CA IXSV) -- 5.2. Batch -- 5.2.1. Urate oxidase (UO) -- 5.3. Dialysis -- 6. Conclusions -- Acknowledgments -- References -- Chapter Three: Prospects for membrane protein crystals in NMX -- 1. Introduction -- 2. Microdialysis crystallization -- 3. Capillary counter-diffusion crystallization -- 4. Crystallization of SERCA by microdialysis and capillary counterdiffusion -- 4.1. Equipment -- 4.2. Chemicals -- 4.3. Protocol -- 4.3.1. Preparation of solubilized SERCA Protein (Ca2E1-AMPPCP form)
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505 |
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|a 4.3.2. Crystallization of the SERCA Ca2E1-AMPPCP form by Microdialysis -- 4.3.3. Crystallization of the SERCA Ca2E1-ADP:AlF4 form by Capillary Counterdiffusion -- 4.3.4. Alternative method for crystallizing SERCA Ca2E1-ADP:AlF4 form by Capillary Counterdiffusion (with gelled batch so ... -- 5. Summary -- Acknowledgment -- Funding information -- References -- Chapter Four: IMAGINE: The neutron protein crystallography beamline at the high flux isotope reactor -- 1. Introduction -- 2. Beamline overview -- 2.1. Neutron source -- 2.2. Beamline optics -- 2.3. Diffractometer
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|a 2.4. Data collection and reduction -- 2.5. Structure refinement -- 3. Sample preparation and ancillary facilities -- 4. Facility access -- 5. Highlights -- 5.1. Neurospora Crassa lytic polysaccharide monooxygenase 9D -- 5.2. H-RAS GTPase -- 5.3. Neutron protein crystallography at cryogenic temperature -- 6. Future development -- 7. Summary -- Acknowledgments -- References -- Chapter Five: The macromolecular neutron diffractometer at the spallation neutron source -- 1. Introduction -- 2. Auto reduction of diffraction data -- 3. Early science highlights -- 4. Human manganese superoxide dismutase
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650 |
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0 |
|a Crystallography.
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650 |
|
0 |
|a Enzymology.
|
650 |
|
0 |
|a Crystallization.
|
650 |
|
0 |
|a Enzymes.
|
650 |
1 |
2 |
|a Crystallography
|0 (DNLM)D003461
|
650 |
1 |
2 |
|a Enzymes
|0 (DNLM)D004798
|
650 |
1 |
2 |
|a Molecular Biology
|x methods
|0 (DNLM)D008967Q000379
|
650 |
2 |
2 |
|a Crystallization
|0 (DNLM)D003460
|
650 |
|
6 |
|a Cristallographie.
|0 (CaQQLa)201-0014886
|
650 |
|
6 |
|a Enzymologie.
|0 (CaQQLa)201-0269407
|
650 |
|
6 |
|a Cristallisation.
|0 (CaQQLa)201-0018431
|
650 |
|
6 |
|a Enzymes.
|0 (CaQQLa)201-0002557
|
650 |
|
7 |
|a crystallization.
|2 aat
|0 (CStmoGRI)aat300214731
|
650 |
|
7 |
|a enzyme.
|2 aat
|0 (CStmoGRI)aat300212647
|
650 |
|
7 |
|a Enzymes
|2 fast
|0 (OCoLC)fst00913605
|
650 |
|
7 |
|a Crystallization
|2 fast
|0 (OCoLC)fst00884644
|
650 |
|
7 |
|a Crystallography
|2 fast
|0 (OCoLC)fst00884652
|
650 |
|
7 |
|a Enzymology
|2 fast
|0 (OCoLC)fst00913635
|
655 |
|
4 |
|a Internet Resources.
|
655 |
|
4 |
|a Index not Present.
|
776 |
0 |
8 |
|i Print version:
|z 0128192143
|z 9780128192146
|w (OCoLC)1109811985
|
830 |
|
0 |
|a Methods in enzymology ;
|v v. 634.
|
856 |
4 |
0 |
|u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/634
|z Texto completo
|