Cargando…

Ubiquitin-dependent protein degradation /

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Hochstrasser, Mark W., 1959- (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Cambridge, MA : Academic Press, 2019.
Edición:First edition.
Colección:Methods in enzymology ; v. 619.
Temas:
Acceso en línea:Texto completo
Texto completo
Tabla de Contenidos:
  • Assays for protein retrotranslocation in ERAD / Sonya Neal, Sascha H. Duttke and Randolph Y. Hampton
  • Methods for measuring misfolded protein clearance in the budding yeast Saccharomyces cerevisiae / Rahul S. Samant and Judith Frydman
  • Methodologies to monitor protein turnover at the inner nuclear membrane / Pei-Ling Tsai, Chenguang Zhao and Christian Schlieker
  • Assays for dissecting the in vitro enzymatic activity of yeast Ubc7 / Bayan Mashahreh, Yuval Reiss, Reuven Wiener and Tommer Ravid
  • Methods for genetic analysis of mammalian ER-associated degradation / Dara E. Leto and Ron R. Kopito
  • A dual system to manipulate protein levels for DNA replication- and cell cycle-related studies / N�estor Garc�ia-Rodr�iguez and Helle D. Ulrich
  • Engineered disulfide crosslinking to measure conformational changes in the 26S proteasome / Randi G. Reed and Robert J. Tomko Jr.
  • Assays for ubiquitin-like protein ligation and proteasome function in archaea / Xian Fu, Zachary Adams and Julie Maupin-Furlow
  • Structural mass spectrometry approaches to study the 20S proteasome / Gili Ben-Nissan, Shay Vimer, Mark Tarnavsky and Michal Sharon
  • Single-molecule methods for measuring ubiquitination and protein stability / Jason Hon and Ying Lu
  • In vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylation / Srinivasan Muniyappan and Byung-Hoon Lee
  • Methods for studying the regulation of membrane traffic by ubiquitin and the ESCRT pathway / Evan L. Guiney, Lu Zhu, Richa Sardana, Scott D. Emr and Matthew G. Baile
  • Detection of ubiquitinated targets in mammalian and Drosophila models / Elena Maspero, Valentina Fajner, Janine Weber and Simona Polo
  • Western blot analysis of the autophagosomal membrane protein LGG-1/LC3 in Caenorhabditis elegans / Alexander Springhorn and Thorsten Hoppe
  • Monitoring stress-induced autophagic engulfment and degradation of the 26S proteasome in mammalian cells / Victoria Cohen-Kaplan, Ido Livneh, Yong Tae Kwon and Aaron Ciechanover.