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Ecometabolomics : metabolic fluxes versus environmental stoichiometry /
'Ecometabolomics: Metabolic Fluxes versus Environmental Stoichiometry' focuses on the interaction between plants -- particularly plants that have vigorous secondary metabolites -- and the environment. The book offers a comprehensive overview of the responses of the metabolome of organisms...
| Clasificación: | Libro Electrónico |
|---|---|
| Autores principales: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
London, United Kingdom ; San Diego, CA, United States :
Academic Press is an imprint of Elsevier,
[2019]
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Ecometabolomics: Metabolic Fluxes Versus Environmental Stoichiometry; Copyright; Dedication; Contents; Preface; Acknowledgements; About the Book; Chapter 1: Introducing Metabolomics; 1.1. Introduction; 1.2. Non-Targeted Identification and Quantification of All Metabolites; 1.2.1. Quality Control Samples; Quality Control Procedures; 1.2.2. Steps Involved in Non-Targeted Metabolomics; Selection of Sample; Sample Treatment and Analytical Conditions; Analytical Method Validation; 1.3. Quantitative Detection of Metabolites; 1.4. Identification and Quantification of Metabolites
- 1.5. Targeted Metabolite Analysis 1.5.1. Metabolic Flux Analysis; 1.6. Metabolite Fingerprinting; 1.6.1. Metabolite Profiling of Plant Tissues Using NMR; 1.7. Metabolite Footprinting; 1.8. Conclusion; References; Further Reading; Chapter 2: An Integrated Approach to Plant Biology via Multi-Analogous Methods; 2.1. Introduction; 2.2. Transcript Profiling; 2.2.1. Library Preparation from RNA Samples; 2.2.2. MicroRNA Profiling; 2.3. Microarray-Based Approaches; 2.3.1. Comparative Genomic Hybridization to Microarrays; 2.3.2. The International HapMap Project; 2.3.3. SNP Genotyping Arrays
- 2.3.4. SNP Genotyping Array Studies 2.4. Sequencing-Based Approaches; 2.4.1. Advantages of RNA-Seq Technology; 2.4.2. Challenges for RNA-Seq Library Construction; 2.5. Differential-Display-Based Approaches; 2.5.1. Amplified Fragment Length Polymorphism; 2.5.2. Advantages of AFLP Analysis; 2.6. Protein Analysis; 2.6.1. Two-Dimensional Gel Electrophoresis; 2.6.2. Capillary IEF; Principle of IEF; Instrumentation of Capillary Iso-electro Focusing; Applications of Capillary IEF; 2.7. Metabolite Analysis; 2.7.1. Metabolic Fingerprinting; 2.7.2. Metabolite Profiling; 2.8. Analytical Techniques
- 2.8.1. Gas Chromatography Coupled With Mass Spectrometry 2.8.2. Nuclear Magnetic Resonance; 2.8.3. Mass Spectrometry; 2.8.4. Liquid Chromatography-Mass Spectrometry; 2.8.5. Capillary Electrophoresis-Mass Spectrometry; 2.8.6. Other Technologies; 2.9. Pattern Recognition; 2.10. Multivariate Statistics Techniques; 2.10.1. Principal Component Analysis; 2.10.2. Partial Least Squares; 2.10.3. Orthogonal Projection to Latent Structures; 2.11. Bioinformatics for Metabolomics; 2.12. Conclusions; References; Further Reading; Chapter 3: Metabolomics Studies of Stress in Plants; 3.1. Introduction
- 3.2. Temperature Stress 3.2.1. Low-Temperature Response; 3.2.2. High-Temperature Stress Response; 3.3. Water and Salt Stress; 3.4. Sulfur and Phosphorus Stress; 3.5. Oxidative Stress; 3.6. Heavy Metal Stress; 3.7. Metabolomic Analysis of Plant-Stress Interactions; 3.8. Metabolic Network Implicated in Stress Signaling; 3.8.1. Functional Significance of ABA in Metabolic Adjustments; 3.8.2. Role of Proline Accumulation in Signaling; 3.8.3. Transcription Factors Regulating Metabolite Levels; 3.9. Conclusion; References; Further Reading; Chapter 4: Climatic Change and Metabolome Fluxes