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Molecular biology techniques : a classroom laboratory manual /
Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergra...
| Clasificación: | Libro Electrónico |
|---|---|
| Autores principales: | , , , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
London, United Kingdom :
Academic Press, an imprint of Elsevier,
2019.
|
| Edición: | Fourth edition. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 003 | OCoLC | ||
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| 008 | 190314s2019 enk ob 001 0 eng d | ||
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| 020 | |a 9780128157756 |q (electronic book) | ||
| 020 | |a 0128157755 |q (electronic book) | ||
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| 020 | |z 0128180242 | ||
| 020 | |z 9780128157749 |q (hardcover) | ||
| 020 | |z 0128157747 |q (hardcover) | ||
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| 050 | 4 | |a QH506 |b .C36 2019eb | |
| 060 | 4 | |a 2019 D-087 | |
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| 082 | 0 | 4 | |a 572.8078 |2 23 |
| 100 | 1 | |a Carson, Sue |q (Susan), |e author. | |
| 245 | 1 | 0 | |a Molecular biology techniques : |b a classroom laboratory manual / |c Susan Carson, Heather B. Miller, D. Scott Witherow, Melissa C. Srougi. |
| 250 | |a Fourth edition. | ||
| 264 | 1 | |a London, United Kingdom : |b Academic Press, an imprint of Elsevier, |c 2019. | |
| 300 | |a 1 online resource | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a data file | ||
| 500 | |a Includes index. | ||
| 588 | 0 | |a Online resource; title from PDF title page (ScienceDirect, viewed March 14, 2019). | |
| 504 | |a Includes bibliographical references and index. | ||
| 520 | |a Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology--or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein. | ||
| 505 | 0 | |a Part I. Manipulation of DNA -- Getting oriented: practicing with micropipettes -- Purification and digestion of plasmid (vector) DNA -- Completion of vector preparation and polymerase chain reaction amplification of egfp -- Preparation on insert DNA (egfp) PCR product -- DNA ligation and transformation of Escherichia coli -- Part II. Screening transformants -- Screening of transformants, part I -- Screening of transformants, part II -- Analysis of DNA sequence from a positive clone -- Part III. Expression, detection, and purification of recombinant proteins from bacteria -- Expressions of fusion protein from positive clones, SDS-PAGE and Western Blot: Part I -- Expressions of fusion protein from positive clones, SDS-PAGE and Western Blot: Part II -- Extraction of recombinant protein from Escherichia coli using a glutathione affinity culture -- Analysis of purification fractions -- Part IV. Analysis of mRNA levels -- Total RNA purification -- Analysis of gst:egfp mRNA levels by RT-qPCR: Part I -- Analysis of gst:egfp mRNA levels by RT-qPCR: Part II -- Analysis of gst:egfp mRNA levels by semiquantitative RT-PCR, Part I -- Analysis of gst:egfp mRNA levels by semiquantitative RT-PCR, Part II -- Part V. Modulation of gene expression -- Culturing mammalian cells -- Transient transfection of mammalian cells -- RNAi-mediated knockdown of EGFP, Part I -- RNAi-mediated knockdown of EGFP, Part II -- RNAi-mediated knockdown of EGFP, Part III -- CRISPR-mediated knockout of EGFP, Part I -- CRISPR-mediated knockout of EGFP, Part II -- Advanced CRISPR, Part I -- Advanced CRISPR, Part II -- Interim laboratory session -- Advanced CRISPR, Part III -- Advanced CRISPR, Part IV -- Advanced CRISPR, Part V -- Advanced CRISPR, Part VI. | |
| 650 | 0 | |a Molecular biology |v Laboratory manuals. | |
| 650 | 0 | |a Genetics |x Technique. | |
| 650 | 1 | 2 | |a Genetic Techniques |0 (DNLM)D005821 |
| 650 | 2 | 2 | |a Molecular Biology |x methods |0 (DNLM)D008967Q000379 |
| 650 | 2 | 2 | |a CRISPR-Cas Systems |0 (DNLM)D064113 |
| 650 | 6 | |a Biologie mol�eculaire |0 (CaQQLa)201-0064024 |v Manuels de laboratoire. |0 (CaQQLa)201-0376994 | |
| 650 | 6 | |a G�en�etique |x Technique. |0 (CaQQLa)201-0068339 | |
| 650 | 6 | |a Syst�emes CRISPR-Cas. |0 (CaQQLa)000313482 | |
| 650 | 7 | |a Genetics |x Technique. |2 fast |0 (OCoLC)fst00940145 | |
| 650 | 7 | |a Molecular biology. |2 fast |0 (OCoLC)fst01024734 | |
| 655 | 2 | |a Laboratory Manual |0 (DNLM)D020484 | |
| 655 | 7 | |a Laboratory manuals. |2 fast |0 (OCoLC)fst01920776 | |
| 655 | 7 | |a Laboratory manuals. |2 lcgft | |
| 655 | 7 | |a Manuels de laboratoire. |2 rvmgf |0 (CaQQLa)RVMGF-000001214 | |
| 700 | 1 | |a Miller, Heather B., |e author. | |
| 700 | 1 | |a Srougi, Melissa C., |e author. | |
| 700 | 1 | |a Witherow, D. Scott, |e author. | |
| 776 | 0 | 8 | |i Print version: |a Carson, Sue (Susan). |t Molecular biology techniques. |b Fourth edition. |d London, United Kingdom : Academic Press, an imprint of Elsevier, 2019 |z 0128180242 |z 9780128180242 |w (OCoLC)1047781545 |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780128180242 |z Texto completo |