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SCIDIR_on1089831122 |
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OCoLC |
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|a 9780128157756
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|a (OCoLC)1089831122
|z (OCoLC)1090911132
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|z (OCoLC)1229477890
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|a QU 25
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|a 572.8078
|2 23
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100 |
1 |
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|a Carson, Sue
|q (Susan),
|e author.
|
245 |
1 |
0 |
|a Molecular biology techniques :
|b a classroom laboratory manual /
|c Susan Carson, Heather B. Miller, D. Scott Witherow, Melissa C. Srougi.
|
250 |
|
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|a Fourth edition.
|
264 |
|
1 |
|a London, United Kingdom :
|b Academic Press, an imprint of Elsevier,
|c 2019.
|
300 |
|
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|a 1 online resource
|
336 |
|
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|a text
|b txt
|2 rdacontent
|
337 |
|
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|a computer
|b c
|2 rdamedia
|
338 |
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|a online resource
|b cr
|2 rdacarrier
|
347 |
|
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|a data file
|
500 |
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|a Includes index.
|
588 |
0 |
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|a Online resource; title from PDF title page (ScienceDirect, viewed March 14, 2019).
|
504 |
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|a Includes bibliographical references and index.
|
520 |
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|a Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology--or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein.
|
505 |
0 |
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|a Part I. Manipulation of DNA -- Getting oriented: practicing with micropipettes -- Purification and digestion of plasmid (vector) DNA -- Completion of vector preparation and polymerase chain reaction amplification of egfp -- Preparation on insert DNA (egfp) PCR product -- DNA ligation and transformation of Escherichia coli -- Part II. Screening transformants -- Screening of transformants, part I -- Screening of transformants, part II -- Analysis of DNA sequence from a positive clone -- Part III. Expression, detection, and purification of recombinant proteins from bacteria -- Expressions of fusion protein from positive clones, SDS-PAGE and Western Blot: Part I -- Expressions of fusion protein from positive clones, SDS-PAGE and Western Blot: Part II -- Extraction of recombinant protein from Escherichia coli using a glutathione affinity culture -- Analysis of purification fractions -- Part IV. Analysis of mRNA levels -- Total RNA purification -- Analysis of gst:egfp mRNA levels by RT-qPCR: Part I -- Analysis of gst:egfp mRNA levels by RT-qPCR: Part II -- Analysis of gst:egfp mRNA levels by semiquantitative RT-PCR, Part I -- Analysis of gst:egfp mRNA levels by semiquantitative RT-PCR, Part II -- Part V. Modulation of gene expression -- Culturing mammalian cells -- Transient transfection of mammalian cells -- RNAi-mediated knockdown of EGFP, Part I -- RNAi-mediated knockdown of EGFP, Part II -- RNAi-mediated knockdown of EGFP, Part III -- CRISPR-mediated knockout of EGFP, Part I -- CRISPR-mediated knockout of EGFP, Part II -- Advanced CRISPR, Part I -- Advanced CRISPR, Part II -- Interim laboratory session -- Advanced CRISPR, Part III -- Advanced CRISPR, Part IV -- Advanced CRISPR, Part V -- Advanced CRISPR, Part VI.
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650 |
|
0 |
|a Molecular biology
|v Laboratory manuals.
|
650 |
|
0 |
|a Genetics
|x Technique.
|
650 |
1 |
2 |
|a Genetic Techniques
|0 (DNLM)D005821
|
650 |
2 |
2 |
|a Molecular Biology
|x methods
|0 (DNLM)D008967Q000379
|
650 |
2 |
2 |
|a CRISPR-Cas Systems
|0 (DNLM)D064113
|
650 |
|
6 |
|a Biologie mol�eculaire
|0 (CaQQLa)201-0064024
|v Manuels de laboratoire.
|0 (CaQQLa)201-0376994
|
650 |
|
6 |
|a G�en�etique
|x Technique.
|0 (CaQQLa)201-0068339
|
650 |
|
6 |
|a Syst�emes CRISPR-Cas.
|0 (CaQQLa)000313482
|
650 |
|
7 |
|a Genetics
|x Technique.
|2 fast
|0 (OCoLC)fst00940145
|
650 |
|
7 |
|a Molecular biology.
|2 fast
|0 (OCoLC)fst01024734
|
655 |
|
2 |
|a Laboratory Manual
|0 (DNLM)D020484
|
655 |
|
7 |
|a Laboratory manuals.
|2 fast
|0 (OCoLC)fst01920776
|
655 |
|
7 |
|a Laboratory manuals.
|2 lcgft
|
655 |
|
7 |
|a Manuels de laboratoire.
|2 rvmgf
|0 (CaQQLa)RVMGF-000001214
|
700 |
1 |
|
|a Miller, Heather B.,
|e author.
|
700 |
1 |
|
|a Srougi, Melissa C.,
|e author.
|
700 |
1 |
|
|a Witherow, D. Scott,
|e author.
|
776 |
0 |
8 |
|i Print version:
|a Carson, Sue (Susan).
|t Molecular biology techniques.
|b Fourth edition.
|d London, United Kingdom : Academic Press, an imprint of Elsevier, 2019
|z 0128180242
|z 9780128180242
|w (OCoLC)1047781545
|
856 |
4 |
0 |
|u https://sciencedirect.uam.elogim.com/science/book/9780128180242
|z Texto completo
|