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High-density sequencing applications in microbial molecular genetics /
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Cambridge, MA :
Academic Press,
2018.
|
| Colección: | Methods in enzymology ;
v. 612. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
MARC
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| 245 | 0 | 0 | |a High-density sequencing applications in microbial molecular genetics / |c edited by Agamemnon J. Carpousis. |
| 264 | 1 | |a Cambridge, MA : |b Academic Press, |c 2018. | |
| 300 | |a 1 online resource | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a data file | ||
| 490 | 1 | |a Methods in enzymology ; |v volume 612 | |
| 504 | |a Includes bibliographical references. | ||
| 588 | 0 | |a Online resource; title from PDF file page (EBSCO, viewed December 5, 2018). | |
| 505 | 0 | |a Front Cover; High-Density Sequencing Applications in Microbial Molecular Genetics; Copyright; Contents; Contributors; Preface; Chapter One: Characterizing the Role of Exoribonucleases in the Control of Microbial Gene Expression: Differential RNA-Seq; 1. Overview of Exoribonucleases; 2. Overview of Differential RNA-Seq; 3. RNA Extraction; 4. Bioinformatics Analysis; 4.1. Mapping of Reads; 4.2. Transcript Expression Quantification; 4.3. Differential Expression Analysis; 4.4. Functional Annotation Analysis; 5. Experimental Validation; 5.1. qPCR; 5.2. Motility Assays; 6. Conclusion | |
| 505 | 8 | |a AcknowledgmentsReferences; Chapter Two: Conformational Studies of Bacterial Chromosomes by High-Throughput Sequencing Methods; 1. Introduction; 1.1. Chromosome Conformation; 1.2. DNA-Protein Interactions; 1.3. Combining NGS to 3C and ChIP Approaches; 2. 3C-Seq: The Protocol; 2.1. Buffers and Reagents; 2.2. Cross-Linking of Cell Cultures; 2.3. 3C Library Preparation; 2.3.1. Cell Lysis; 2.3.2. RE Digestion; 2.3.3. Ligation; 2.3.4. DNA Purification; 2.4. Notes; 3. ChIP-Seq: The Protocol; 3.1. Buffers and Reagents; 3.2. Cross-Linking of Cell Cultures; 3.3. Cell Lysis | |
| 505 | 8 | |a 3.4. Batch Immunoprecipitation of FLAG Fusions Proteins3.5. Elution of the Immunoprecipitated Protein; 3.6. Purification of the Coimmunoprecipitated DNA; 3.7. Notes; 4. Data Processing and Analysis; 4.1. Analysis of 3C-Seq Data; 4.2. Analysis of ChIP-Seq Data; 5. Conclusion; Acknowledgments; References; Chapter Three: Large-Scale Measurement of mRNA Degradation in Escherichia coli: To Delay or Not to Delay; 1. Introduction; 2. Measuring mRNA Degradation Rates; 2.1. Methods; 2.2. Rifampicin; 2.3. Practical Considerations; 3. Genome-Wide mRNA Quantification | |
| 505 | 8 | |a 3.1. mRNA Extraction and Quantification3.2. Normalization; 4. mRNA Half-Life Estimation; 4.1. Decay Models; 4.2. Accuracy Criteria of the Estimation; 5. Effect of the Delay Before the Onset of Exponential Decay on mRNA Half-Life Estimation; 5.1. Datasets of mRNA Levels Over the Time; 5.2. Estimations of the Delay Before the Onset of the mRNA Exponential Decay; 5.3. Comparison of mRNA Half-Lives Estimated With and Without a Delay; 5.4. Correlation Between mRNA Half-Lives Estimated With and Without a Delay; 5.5. Influence of the Delay Extent | |
| 505 | 8 | |a 5.6. Validation on RT-qPCR mRNA Quantification Datasets6. Concluding Remarks; Acknowledgments; References; Chapter Four: FASTBAC-Seq: Functional AnalysiS of Toxin-Antitoxin Systems in BACteria by Deep Sequencing; 1. Introduction; 2. Materials; 2.1. Molecular Biology Equipment; 2.2. General Equipment for Bacterial Cultures; 2.3. E. coli-Specific Equipment; 2.4. H. pylori-Specific Equipment; 2.5. Molecular Biology Reagents; 2.6. E. coli Strains and Cell Culture; 2.7. H. pylori Strains and Cell Culture | |
| 650 | 0 | |a Molecular genetics. | |
| 650 | 0 | |a Microbial genetics. | |
| 650 | 1 | 2 | |a High-Throughput Nucleotide Sequencing |x methods |0 (DNLM)D059014Q000379 |
| 650 | 1 | 2 | |a Sequence Analysis, RNA |0 (DNLM)D017423 |
| 650 | 2 | 2 | |a Genetics, Microbial |0 (DNLM)D005827 |
| 650 | 2 | 2 | |a Metagenomics |x methods |0 (DNLM)D056186Q000379 |
| 650 | 2 | 2 | |a Molecular Biology |0 (DNLM)D008967 |
| 650 | 6 | |a G�en�etique mol�eculaire. |0 (CaQQLa)201-0008935 | |
| 650 | 6 | |a G�en�etique microbienne. |0 (CaQQLa)201-0008605 | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Evolution. |2 bisacsh | |
| 650 | 7 | |a Microbial genetics. |2 fast |0 (OCoLC)fst01019500 | |
| 650 | 7 | |a Molecular genetics. |2 fast |0 (OCoLC)fst01024797 | |
| 655 | 4 | |a Internet Resources. | |
| 655 | 4 | |a Index not Present. | |
| 700 | 1 | |a Carpousis, Agamemnon J., |e editor. | |
| 776 | 0 | 8 | |i Print version: |a High-density sequencing applications in microbial molecular genetics. |b First edition. |d Cambridge, MA : Academic Press, an imprint of Elsevier, 2018 |z 9780128159934 |w (OCoLC)1035514436 |
| 830 | 0 | |a Methods in enzymology ; |v v. 612. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/612 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/bookseries/methods-in-enzymology/vol/612/suppl/C |z Texto completo |