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Mitosis and meiosis. Part A /
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Camridge, MA :
Academic Press,
2018.
|
| Colección: | Methods in cell biology ;
v. 144. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
MARC
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| 245 | 0 | 0 | |a Mitosis and meiosis. |n Part A / |c edited by Helder Maiato, Melina Schuh. |
| 264 | 1 | |a Camridge, MA : |b Academic Press, |c 2018. | |
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| 490 | 1 | |a Methods in cell biology ; |v volume 144 | |
| 588 | 0 | |a Online resource; title from PDF title page (ScienceDirect, viewed May 30, 2018). | |
| 505 | 0 | |a Front Cover; Mitosis and Meiosis Part A; Copyright; Contents; Contributors; Preface; Chapter 1: Assays for the spindle assembly checkpoint in cell culture; 1. Introduction; 2. Choice of Fluorescent Protein Tag; 3. Choice of Microscope; 4. Assays; 5. Materials; Acknowledgments; References; Chapter 2: Quantitative methods to measure aneuploidy and chromosomal instability; 1. Introduction; 2. Immunofluorescence Assay for Lagging Chromosomes in Anaphase; 2.1. Instrumentation; 2.2. Cell Culture Conditions; 2.3. Antibodies; 2.4. Immunofluorescence; 2.5. Analysis; 2.6. Alternative Method | |
| 505 | 8 | |a 3. Chromosome Missegregation Assay3.1. Instrumentation; 3.2. Cell Culture Conditions; 3.3. FISH Probes; 3.4. Preparation of Mitotic Cells; 3.5. FISH Procedure; 3.6. Analysis; 3.7. Alternative Method; 4. Aneuploid Cell Survival Assay; 4.1. Instrumentation; 4.2. Cell Culture Conditions; 4.3. FISH Probes; 4.4. Preparation of Interphase Cells; 4.5. FISH Procedure; 4.6. Analysis; 4.7. Alternative Method; 5. Concluding Remarks; Acknowledgments; References; Chapter 3: Dissecting the role of the tubulin code in mitosis; 1. Introduction; 1.1. What Is the Tubulin Code?; 1.2. Tubulin Isotypes | |
| 505 | 8 | |a 1.3. Tubulin PTMs1.4. How Is the Tubulin Code Read?; 2. Modulation of the Detyrosination/Tyrosination Cycle in Mammalian Cells; 2.1. Cell Culture; 2.2. Transient Overexpression of TTL; 2.3. Depletion of TTL Using Small Interference RNAs (siRNAs); 2.4. Knockout of TTL Using CRISPR/Cas9; 2.4.1. Purchase oligos; 2.4.2. Oligo annealing and cloning into viral transfer vectors; 2.4.3. Transformation and selection; 2.4.4. Lentivirus production; 2.4.5. Transduction of lentivirus to target cells; 2.4.6. Selection of knockout cells; 2.5. Transient Overexpression of VASH1 and VASH2 | |
| 505 | 8 | |a 2.6. Generation of Cell Lines Stably Expressing FLAG-VASH1 and FLAG-VASH22.6.1. Retrovirus production; 2.6.2. Transduction of retrovirus to target cells and selection of overexpressing cells; 2.7. Knockout of VASH1 and VASH2 Using CRISPR/Cas9; 2.7.1. Purchase oligos; 2.7.2. Oligo annealing and cloning into viral transfer vectors; 2.7.3. Lentivirus production; 2.7.4. Transduction of lentivirus to target cells and selection of knockout cells; 2.8. Depletion of Endogenous �I�-Tubulin Isotypes Using siRNA; 2.9. Transient Overexpression of Tyrosinated, Detyrosinated and Delta2 Forms of TUBA1B | |
| 505 | 8 | |a 2.9.1. Site-directed mutagenesis of mammalian expression vectors2.9.2. Altering the cDNA sequences to confer resistance to siRNA depletion; 2.9.3. Transient expression of tyrosinated, detyrosinated, and Delta2 forms of TUBA1B; 2.10. Generation of a Cell Line Stably Expressing H2B-mRFP and Tyrosinated, Detyrosinated, or Delta2 Forms of TUBA1B; 2.10.1. Cloning of TUBA1B cDNA into lentiviral transfer vectors; 2.10.2. Deletion of EGFP-tag from lentiviral vectors expressing TUBA1B by PCR; 2.10.3. Production of lentivirus and transduction to target cells | |
| 650 | 0 | |a Mitosis. | |
| 650 | 0 | |a Meiosis. | |
| 650 | 2 | |a Mitosis |0 (DNLM)D008938 | |
| 650 | 2 | |a Meiosis |0 (DNLM)D008540 | |
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| 650 | 6 | |a M�eiose. |0 (CaQQLa)201-0093331 | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Anatomy & Physiology. |2 bisacsh | |
| 650 | 7 | |a Meiosis |2 fast |0 (OCoLC)fst01015610 | |
| 650 | 7 | |a Mitosis |2 fast |0 (OCoLC)fst01024031 | |
| 700 | 1 | |a Maiato, Helder, |e editor. | |
| 700 | 1 | |a Schuh, Melina, |e editor. | |
| 776 | 0 | 8 | |i Print version: |t Mitosis and meiosis. Part A. |d Camridge, MA : Academic Press, 2018 |z 0128141441 |z 9780128141441 |w (OCoLC)1020635024 |
| 830 | 0 | |a Methods in cell biology ; |v v. 144. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/0091679X/144 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780128141441 |z Texto completo |