In vitro toxicology /

In Vitro Toxicology details the protocols and methods of in vitro testing, highlighting the usefulness of models, methods and the cost-effectiveness and reproducibility of such methodologies. The current approaches and strategies required to develop an easy, reliable, validated and high throughput s...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Dhawan, Alok (Editor ), Kwon, Seok (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: London : Academic Press, an imprint of Elsevier, [2017]
Temas:
Toxicology.
Toxicology
Toxicologie.
toxicology.
MEDICAL > Pharmacology.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Front Cover
  • In Vitro Toxicology
  • In Vitro Toxicology
  • Copyright
  • Contents
  • List of Contributors
  • Editors Biographies
  • Foreword
  • Preface
  • Acknowledgments
  • 1
  • Development of In Vitro Toxicology: A Historic Story
  • INTRODUCTION
  • WHAT IN VITRO TOXICOLOGY AND HOW IT CAME INTO BEING?
  • WHY IN VITRO NOT IN VIVO?
  • THE REGULATORY CONTROL AND POLICY MAKERS
  • PROJECTS AND DEVELOPMENTS
  • SOME OF THE VALIDATED IN VITRO ALTERNATIVES AND METHODS
  • THE AVAILABLE IN VITRO MODELS SYSTEMS
  • Explant/Organ Culture
  • Cell Culture
  • Primary Cell CultureImmortalized Cell Lines
  • Organotypic Cultures
  • Stem Cells and Induced Pluripotent Stem Cells
  • Organoid Culture
  • BASAL TO ORGAN-SPECIFIC ENDPOINTS OF IN VITRO TOXICOLOGY
  • Basal Toxicity or Cytotoxicity
  • Tetrazolium Bromide Salt Assay
  • Lactate Dehydrogenase Assay
  • Neutral Red Uptake Assay
  • Acetoxymethyl Diacetylester of Calcein Cytotoxicity Assay
  • Adenosine Triphosphate Quantification and Adenosine Triphosphate/Adenosine Diphosphate Ratio Assay
  • Genotoxicity
  • CELLS TO 3D ORGANOIDS: IN VITRO ORGAN SYSTEM TOXICOLOGY
  • Hepatoxicity In VitroNeurotoxicity In Vitro
  • Nephrotoxicity In Vitro
  • Cardiotoxicity In Vitro
  • Nanotoxicology In Vitro
  • SUMMARY
  • SUGGESTED WEB PORTALS FOR FURTHER READING
  • REFERENCES
  • 2
  • Principles for In Vitro Toxicology
  • INTRODUCTION
  • GUIDELINES GOVERNING THE IN VITRO METHODS FOR TOXICITY TESTING
  • OPTIMIZATION OF CELL CULTURE
  • MODELS OF IN VITRO TOXICITY
  • Explant Culture
  • Organotypic Culture
  • Cell Cultures
  • Three Dimensional Culture Systems
  • DEVELOPMENT OF BIOMARKERS FOR IN VITRO TOXICITY EVALUATION
  • ASSAYS FOR SCREENING OF TOXICANTSAmes Test
  • The Hen�a#x80;#x99;s Egg Test�a#x80;#x93;Chorio Allantoic Membrane Test
  • Corrosivity Assays
  • 3T3 Neutral Red Uptake Test
  • Tetrazolium Salt, 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide Assay
  • Alamar Blue
  • The Bovine Cornea Opacity/Permeability Test
  • DOSE�a#x80;#x93;RESPONSE RELATIONSHIP
  • Evaluation of DR Relationship
  • DATA INTERPRETATION
  • VALIDATION OF IN VITRO TOXICOLOGY TEST METHODS
  • THE �a#x80;#x9C;OMICS�a#x80;#x9D; APPROACH
  • CONCLUSION
  • ACKNOWLEDGMENTS
  • REFERENCES
  • 3
  • Models and Methods for In Vitro ToxicityINTRODUCTION
  • NEED OF IN VITRO MODELS FOR TOXICITY ASSESSMENT
  • VALIDATION OF IN VITRO TEST METHODS
  • Criteria for Validation
  • Criteria for Regulatory Acceptance
  • MODELS FOR IN VITRO TOXICITY ASSESSMENT
  • In Vitro Models for Cytotoxicity Studies
  • In Vitro Models for Specific Toxicity
  • In Vitro Models for Liver Toxicity
  • In Vitro Models for Lung Toxicity
  • In Vitro Models for Neurotoxicity
  • In Vitro Models for Immunotoxicity
  • METHODS EMPLOYED FOR IN VITRO TOXICITY ASSESSMENT

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