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Harnessing the power of viruses /
Harnessing the Power of Viruses explores the application of scientific knowledge about viruses and their lives to solve practical challenges and further advance molecular sciences, medicine and agriculture. The book contains virus-based tools and approaches in the fields of: i) DNA manipulations in...
| Clasificación: | Libro Electrónico |
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| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
London :
Academic Press, an imprint of Elsevier,
[2017]
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- 1
- INTRODUCTION TO VIRAL STRUCTURE, DIVERSITY AND BIOLOGY ; 1.2 OVERVIEW OF VIRAL STRUCTURE: CAPSIDS, GENOMES, AND ENVELOPES ; 1.2.1 Capsids ; 1.2.2 Genomes ; 1.2.3 Envelopes ; 1.2.4 Additional Virion Components ; 1.3 VIRUS DIVERSITY AND CLASSIFICATION: ORIGIN OF VIRUSES ; 1.4 APPROACHES TO VISUALIZE VIRUSES ; 1.5 VIRAL LIFE CYCLES: LYTIC VERSUS LATENT ; 1.5.1 Adsorption/Attachment
- 1.5.2 Penetration/Viral Entry 1.5.3 Biosynthesis ; 1.5.4 Assembly ; 1.5.5 Release ; Further Reading ; 2
- Viral Tools for In Vitro Manipulations of Nucleic Acids: Molecular Cloning ; 2.1 Overview of Molecular Cloning ; 2.1.1 Structure and Chemical Composition of Nucleic Acids: Flow of Genetic Information ; 2.1.2 Principles of Molecular Cloning ; 2.2 Vectors and Other Nonenzyme Tools Derived From Viruses ; 2.2.1 Viral Vectors ; 2.2.2 Virus-Based DNA Ladders ; 2.2.3 Phage-Resistant Escherichia coli Strains
- 2.3 Virus-Related Enzymes in Recombinant DNA Technology 2.3.1 Restriction Enzymes and Viral Nucleases ; 2.3.1.1 Restriction Enzymes ; 2.3.1.2 Restriction Enzyme Applications ; 2.3.1.3 Sequence-Nonspecific DNA Nucleases ; 2.3.2 Replication-Related Enzymes ; 2.3.2.1 DNA Polymerases ; 2.3.2.2 Reverse Transcriptases ; 2.3.2.3 DNA Ligases ; 2.3.2.4 Topoisomerases ; 2.3.2.5 Single-Stranded DNA Proteins ; 2.3.3 DNA-Modifying Enzymes ; 2.3.3.1 Polynucleotide Kinase ; 2.3.3.2 Methyltransferases ; 2.4 Viral Tools for Manipulating RNA In Vitro
- 2.4.1 RNA Polymerases 2.4.2 Vaccinia Virus RNA Capping System ; 2.4.3 RNA Ligases ; Further Reading ; 3
- Viral Tools for Genome Manipulations In Vivo ; 3.1 CONVENTIONAL APPROACHES TO GENOME MANIPULATIONS ; 3.2 VIRUS-BASED RECOMBINATION SYSTEMS AND THEIR APPLICATIONS ; 3.2.1 Virus-Driven Recombination Overview ; 3.2.2 Lambda Red Recombineering ; 3.2.3 Cre/LoxP Recombination System ; 3.2.4 Phage Integrases ; 3.3 GENOME EDITING ; 3.3.1 Genome Editing Overview ; 3.3.1.1 Meganucleases ; 3.3.1.2 Zinc-Finger Nucleases
- 3.3.1.3 Transcription Activatora-Like Endonucleases 3.3.1.4 The CRISPR/Cas9 system ; 3.3.2 Key Applications of Nuclease-Based Editing ; 3.3.3 Biology of the CRISPR/Cas-Based Adaptive Immune System in Prokaryotes: The Big Picture ; 3.3.3.1 CRISPR-Based Adaptive Immunity: Components and Mechanism ; 3.4 RNA INTERFERENCE ; Further Reading ; 4
- Viral Tools for Protein Expression and Purification ; 4.1 Overview of Protein Expression: Challenges and Limitations ; 4.2 Viral Components Utilized in Protein Expression Systems