The handbook of histopathological practices in aquatic environments : guide to histology for environmental toxicology /

"[This work] offers readers in aquatic biology and other water-based environmental sciences a comprehensive resource on histopathology, which is a key tool in the growing field of ecotoxicology. This work brings together the necessary knowledge, from sample preparation, to trait identification,...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Costa, Pedro Moura (Autor)
Formato: Electrónico eBook
Idioma:Inglés
Publicado: London : Academic Press, an imprint of Elsevier, 2017.
Temas:
Water > Pollution > Toxicology.
Histology, Pathological.
Histology.
Environmental toxicology.
Ecotoxicology
Water Pollution > analysis
Aquatic Organisms
Histology
Pathology
Histopathologie.
Histologie.
�Ecotoxicologie.
SCIENCE > Life Sciences > Anatomy & Physiology.
Histology, Pathological
Water > Pollution > Toxicology
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Chapter 1. Introduction
  • Chapter 2. Fundamental concepts
  • Chapter 3. Sample preparation
  • Chapter 4. Staining protocols
  • Chapter 5. Microphotography and image processing: Creating artwork
  • Chapter 6. Identification of major histopathological traits
  • Chapter 7. Scoring and data processing
  • Chapter 8. Common problems and troubleshooting
  • Appendix A. Fixative solutions
  • Appendix B. Dyes
  • Appendix C. Accessory solutions
  • Appendix D. Rapid protocols
  • Appendix E. Accessory tables
  • References
  • Index.
  • Front Cover; The Handbook of Histopathological Practices in Aquatic Environments; Copyright Page; Contents; Foreword; Preface; Acknowledgments; List of Abbreviations and Acronyms; 1 Introduction; 1.1 A Brief Account on the History and Purpose of Histology and Histopathology in Aquatic Organisms; 1.2 Histopathological Traits as Biomarkers in Marine Environmental Science; 1.3 Target Species and Organ: The Importance of Understanding Toxicological Pathways; 1.4 Histopathology Links Many Levels of Biological Organization: The Systems Biology Approach; 1.5 How to Use This Book
  • 2 Fundamental Concepts2.1 Procedural Overview; 2.2 Basic Equipment and Material; 2.2.1 Essential Equipment; 2.2.2 Glass and Plasticware for Sample Preparation; 2.2.3 Microscope slides, Coverslips, and Adhesives; 2.2.4 Microtome Knives and Blades; 2.3 Important Considerations on Solvents and Reagents; 2.3.1 Fixatives; 2.3.2 Embedding Media; 2.3.3 Histological Dyes; 2.3.4 Waters; 2.3.5 Alcohols and Other Solvents; 2.3.6 Buffer Systems; 2.3.7 Mounting Media; 2.4 Safety Precautions; 3 Sample Preparation; 3.1 Tissue Preparation and Fixation; 3.1.1 Harvesting Material; 3.1.2 Fixation
  • 3.1.3 Additional Treatments3.2 Paraffin-Embedded Samples; 3.2.1 General Procedure for Paraffin Infiltration and Embedding; 3.2.2 Casting Paraffin Blocks; 3.2.3 Sectioning Paraffin Sections With a Rotary Microtome; 3.2.4 Staining Paraffin-Embedded Samples; 3.3 Procedures for Resin Embedding; 3.3.1 Embedding Samples in Resins; 3.3.2 Obtaining Semithin and Thin Section From Resin-Embedded Samples; 3.3.3 Staining and Contrasting Resin Sections; 4 Staining Protocols; 4.1 General Histological Stains; 4.1.1 Hematoxylin and Eosin; 4.1.1.1 Introduction; 4.1.1.2 Procedure; 4.1.2 Toluidine Blue
  • 4.1.2.1 Introduction4.1.2.2 Procedure; 4.2 Histochemistry of Lipids and Sugars; 4.2.1 Alcian Blue/Nuclear Fast Red Stain for Acidic Polysaccharides; 4.2.1.1 Introduction; 4.2.1.2 Procedure; 4.2.2 Lipid Staining With Osmium Tetroxide; 4.2.2.1 Introduction; 4.2.2.2 Procedure; 4.2.3 Periodic Acid/Schiff's Stain for Polysaccharides; 4.2.3.1 Introduction; 4.2.3.2 Procedure; 4.2.4 Sudan Black for Lipids; 4.2.4.1 Introduction; 4.2.4.2 Procedure; 4.3 Peptide Histochemistry; 4.3.1 Coomassie Blue Stain for Proteins; 4.3.1.1 Introduction; 4.3.1.2 Procedure
  • 4.4 Histochemistry of Metals, Metalloids, and Metallic Compounds4.4.1 Some Important Considerations on Metal Histochemistry; 4.4.2 Castel's Method for Arsenic; 4.4.2.1 Introduction; 4.4.2.2 Procedure; 4.4.3 Perl's Prussian Blue for Hemosiderin; 4.4.3.1 Introduction; 4.4.3.2 Procedure; 4.4.4 Rubeanic Acid Method for Copper; 4.4.4.1 Introduction; 4.4.4.2 Procedure; 4.4.5 von Kossa's Stain for Calcium; 4.4.5.1 Introduction; 4.4.5.2 Procedure; 4.5 Stains for Microorganisms; 4.5.1 Gram's Stain for Paraffin Sections; 4.5.1.1 Introduction; 4.5.1.2 Procedure; 4.5.2 Staining Acid-Fast Microorganisms

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