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SCIDIR_on1003131133 |
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OCoLC |
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20231120010216.0 |
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m o d |
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cr cnu|||unuuu |
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170907s2017 maua o 001 0 eng d |
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|a N$T
|b eng
|e rda
|e pn
|c N$T
|d IDEBK
|d EBLCP
|d OPELS
|d N$T
|d BUF
|d OCLCF
|d OCLCO
|d SCB
|d BRX
|d OCLCQ
|d UKMGB
|d OCLCQ
|d OCLCO
|d OCLCA
|d OCLCQ
|d OCLCO
|d OCLCQ
|d CASUM
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|a GBB7F3171
|2 bnb
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|a 018484080
|2 Uk
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|a 9780128119389
|q (electronic bk.)
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|a 0128119381
|q (electronic bk.)
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|z 9780128119440
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|a (OCoLC)1003131133
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|a QP552.I7
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|a SCI
|x 007000
|2 bisacsh
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|a 572/.7
|2 23
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|a Fe-S cluster enzymes.
|n Part A /
|c edited by Sheila S. David.
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| 250 |
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|a First edition.
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| 264 |
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1 |
|a Cambridge, MA :
|b Academic Press, an imprint of Elsevier,
|c 2017.
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| 264 |
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4 |
|c �2017
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| 300 |
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|a 1 online resource (xv, 425 pages) :
|b illustrations (some color)
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in enzymology ;
|v Volume 595
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0 |
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|a Front Cover; Fe-S Cluster Enzymes Part A; Copyright; Contents; Contributors; Preface; Chapter One: Genetic, Biochemical, and Biophysical Methods for Studying Fe-S Proteins and Their Assembly; 1. Fe-S Cluster Biology; 2. Genetic Approaches and Strategies; 2.1. Genetic Selection and/or Screens Based on Fe-S Biogenesis Defects; 2.2. The Use of Conditional Alleles; 2.3. Genetic Approaches to Investigate Functional Redundancy; 2.4. Suppressor-Based Strategies; 2.5. trans-Dominant Mutations; 2.6. E. coli Strains of Particular Interest for Fe-S Cluster Biogenesis Studies
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| 505 |
8 |
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|a 2.6.1. The eSUF and eISC Strains2.6.2. The Hpx Strain; 2.6.3. The Iron-Minus Strain; 2.7. Construction of Frataxin Models; 3. Biochemistry of Fe-S Clusters; 3.1. Purification of Fe-S Cluster-Containing Proteins; 3.2. Characterization of Fe-S Proteins; 4. Spectroscopy of Fe-S Proteins; 4.1. UV-Visible Absorption Spectroscopy; 4.2. M�ossbauer Spectroscopy; 4.3. Electronic Paramagnetic Resonance Spectroscopy; 4.4. Hyperfine Sublevel Correlation Spectroscopy; 4.5. Raman Resonance Spectroscopy; 4.6. Circular Dichroism; 4.7. Nuclear Magnetic Resonance Spectroscopy; Acknowledgments; References
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| 505 |
8 |
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|a Chapter Two: De Novo Design of Iron-Sulfur Proteins1. Introduction; 2. [4Fe-4S]; 3. [3Fe-4S] Clusters; 4. [2Fe-2S] Clusters; 5. [Fe8S7] Clusters; 6. Protocols; 6.1. Diiron Hexacarbonyl Cluster Incorporation Into Peptide (Roy et al., 2012); 6.2. [4Fe-4S] Cluster Incorporation Into Peptide; 6.3. EPR [4Fe-4S] Sample Preparation; 6.4. Hydrogen Production With Diiron Hexacarbonyl; 6.5. Electrochemical Measurements; References; Chapter Three: In Vitro Studies of Cellular Iron-Sulfur Cluster Biosynthesis, Trafficking, and Transport; 1. Introduction
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|a 2. Preparation of Fe-S Clusters and Kinetic Determination of Fe-S Cluster Transfer Reactions2.1. Na6[Fe2S2](GS)4 Synthesis; 2.1.1. Equipment; 2.1.2. Buffers and Reagents; 2.1.3. Procedure; 2.1.4. Notes; 2.2. Cluster Uptake and Protein-Protein Transfer Reactions; 2.2.1. Equipment; 2.2.2. Buffers and Reagents; 2.2.3. Procedure; 2.2.4. Notes; 2.3. Glutathione Extraction; 2.3.1. Equipment; 2.3.2. Buffers and Reagents; 2.3.3. Procedure; 2.3.4. Notes; 3. Fe-S Cluster Stimulation and Transport in Liposome Assays; 3.1. ATPase Stimulation Assays; 3.1.1. Equipment; 3.1.2. Buffers and Reagents
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| 505 |
8 |
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|a 3.1.3. Procedure3.1.4. Notes; 3.2. Liposome Synthesis and Protein Incorporation; 3.2.1. Equipment; 3.2.2. Buffers and Reagents; 3.2.3. Procedure; 3.2.4. Notes; 3.3. Cluster Transfer Monitored by Tiron Assay and Flow Cytometry; 3.3.1. Equipment; 3.3.2. Buffers and Reagents; 3.3.3. Procedure; 3.3.4. Notes; 4. Summary and Conclusions; References; Chapter Four: Combined Biochemical, Biophysical, and Cellular Methods to Study Fe-S Cluster Transfer and Cytosolic Aconit ... ; 1. Introduction; 2. In Vitro Fe-S Cluster Transfer From mNT to a Model Recipient; 2.1. Purifications of mNT and FDX
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| 500 |
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|a Includes indexes.
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| 588 |
0 |
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|a Online resource; title from PDF title page (ScienceDirect, viewed September 13, 2017).
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| 650 |
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|a Iron-sulfur proteins.
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| 650 |
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2 |
|a Iron-Sulfur Proteins
|0 (DNLM)D007506
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| 650 |
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6 |
|a Ferrosulfoprot�eines.
|0 (CaQQLa)201-0070150
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| 650 |
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7 |
|a SCIENCE
|x Life Sciences
|x Biochemistry.
|2 bisacsh
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| 650 |
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7 |
|a Iron-sulfur proteins
|2 fast
|0 (OCoLC)fst01721284
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| 655 |
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|a Electronic books.
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| 655 |
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4 |
|a Internet Resources.
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| 655 |
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4 |
|a Charts.
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| 700 |
1 |
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|a David, Sheila S.,
|e editor.
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| 776 |
0 |
8 |
|i Print version :
|z 9780128119440
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| 830 |
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0 |
|a Methods in enzymology ;
|v v. 595.
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| 856 |
4 |
0 |
|u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/595
|z Texto completo
|
