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A structure-function toolbox for membrane transporter and channels /

New chapters in the updated serial include Cryo-EM on membrane proteins embedded in nanodics, Solid-Supported membrane-based electrophysiology on membrane transporters and channels, Saposin-lipoprotein scaffolds for structure determination of membrane transporters and channels, Single-molecule FRET...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Ziegler, Christine (Biochemist) (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Cambridge, MA : Academic Press is an imprint of Elsevier, 2017.
Edición:First edition
Colección:Methods in enzymology ; v. 594.
Temas:
Acceso en línea:Texto completo
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Tabla de Contenidos:
  • Chapter 1: Lipid Nanodiscs as a Tool for High-Resolution Structure Determination of Membrane Proteins by Single-Particle Cryo-EM / Rouslan Efremov, Christos Gatsogiannis and Stefan Raunser
  • Chapter 2: SSM-Based Electrophysiology for Transporter Research / Andre Bazzone, Maria Barthmes and Klaus Fendler
  • Chapter 3: Saposin-Lipoprotein Scaffolds for Structure Determination of Membrane Transporters / Joseph Lyons, Andreas B�ggild, Poul Nissen and Jens Frauenfeld
  • Chapter 4: Single-Molecule Fluorescence Studies of Membrane Transporters Using Total Internal Reflection Microscopy / Joris M.H. Goudsmits, Antoine M. van Oijen and Dirk J. Slotboom
  • Chapter 5: The MEMbrane Protein Single ShoT Amplification Recipe: MemStar / Aziz Abdul-Qureshi, Pascal F. Meier, Chiara Lee and David Drew
  • Chapter 6: Efficient Screening and Optimization of Membrane Protein Production in Escherichia coli / Jacopo Marino, Katharina Holzh�uter, Benedikt Kuhn and Eric R. Geertsma
  • Chapter 7: Pinning Down the Mechanism of Transport: Probing the Structure and Function of Transporters Using Cysteine Cross-Linking and Site-Specific Labeling / Christopher Mulligan and Joseph A. Mindell
  • Chapter 8: Ion Channel Conformation and Oligomerization Assessment by Site-Directed Spin Labeling and Pulsed-EPR / Christos Pliotas
  • Chapter 9: Strategy for the Thermostabilization of an Agonist-Bound GPCR Coupled to a G Protein / Annette Strege, Byron Carpenter, Patricia C. Edwards and Christopher G. Tate.