A structure-function toolbox for membrane transporter and channels /
New chapters in the updated serial include Cryo-EM on membrane proteins embedded in nanodics, Solid-Supported membrane-based electrophysiology on membrane transporters and channels, Saposin-lipoprotein scaffolds for structure determination of membrane transporters and channels, Single-molecule FRET...
Clasificación: | Libro Electrónico |
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Otros Autores: | |
Formato: | Electrónico eBook |
Idioma: | Inglés |
Publicado: |
Cambridge, MA :
Academic Press is an imprint of Elsevier,
2017.
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Edición: | First edition |
Colección: | Methods in enzymology ;
v. 594. |
Temas: | |
Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- Chapter 1: Lipid Nanodiscs as a Tool for High-Resolution Structure Determination of Membrane Proteins by Single-Particle Cryo-EM / Rouslan Efremov, Christos Gatsogiannis and Stefan Raunser
- Chapter 2: SSM-Based Electrophysiology for Transporter Research / Andre Bazzone, Maria Barthmes and Klaus Fendler
- Chapter 3: Saposin-Lipoprotein Scaffolds for Structure Determination of Membrane Transporters / Joseph Lyons, Andreas B�ggild, Poul Nissen and Jens Frauenfeld
- Chapter 4: Single-Molecule Fluorescence Studies of Membrane Transporters Using Total Internal Reflection Microscopy / Joris M.H. Goudsmits, Antoine M. van Oijen and Dirk J. Slotboom
- Chapter 5: The MEMbrane Protein Single ShoT Amplification Recipe: MemStar / Aziz Abdul-Qureshi, Pascal F. Meier, Chiara Lee and David Drew
- Chapter 6: Efficient Screening and Optimization of Membrane Protein Production in Escherichia coli / Jacopo Marino, Katharina Holzh�uter, Benedikt Kuhn and Eric R. Geertsma
- Chapter 7: Pinning Down the Mechanism of Transport: Probing the Structure and Function of Transporters Using Cysteine Cross-Linking and Site-Specific Labeling / Christopher Mulligan and Joseph A. Mindell
- Chapter 8: Ion Channel Conformation and Oligomerization Assessment by Site-Directed Spin Labeling and Pulsed-EPR / Christos Pliotas
- Chapter 9: Strategy for the Thermostabilization of an Agonist-Bound GPCR Coupled to a G Protein / Annette Strege, Byron Carpenter, Patricia C. Edwards and Christopher G. Tate.