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DNA repair enzymes : structure, biophysics, and mechanism /
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Cambridge, MA :
Academic Press is an imprint of Elsevier,
2017.
|
| Edición: | First edition. |
| Colección: | Methods in enzymology ;
v. 592. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
MARC
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| 003 | OCoLC | ||
| 005 | 20231120010206.0 | ||
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| 007 | cr cnu|||unuuu | ||
| 008 | 170630s2017 mau o 001 0 eng d | ||
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| 020 | |z 9780128125151 |q (print) | ||
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| 072 | 7 | |a SCI |x 007000 |2 bisacsh | |
| 082 | 0 | 4 | |a 572.86 |2 23 |
| 245 | 0 | 0 | |a DNA repair enzymes : |b structure, biophysics, and mechanism / |c edited by Brandt F. Eichman. |
| 250 | |a First edition. | ||
| 264 | 1 | |a Cambridge, MA : |b Academic Press is an imprint of Elsevier, |c 2017. | |
| 300 | |a 1 online resource | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a data file | ||
| 490 | 1 | |a Methods in enzymology ; |v volume 592 | |
| 500 | |a Includes indexes. | ||
| 588 | 0 | |a Online resource; title from PDF title page (ScienceDirect, viewed July 5, 2017). | |
| 505 | 0 | |a 1. MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes / Jill O. Fuss -- 1. Introduction -- 2. Entry Cloning Into MacroBac Series 11 or 438 by LIC -- 3. Building Complexes by Restriction and Ligation: MacroBac Series 11 -- 4. Building Complexes by LIC: MacroBac Series 438 -- 5. Bacmid Preparation and Virus Production -- 6. Protein Expression and Viral Stock Maintenance -- 7. Trouble Shooting -- 8. Future Considerations -- Acknowledgments -- References -- 2. Production and Assay of Recombinant Multisubunit Chromatin Remodeling Complexes / Dale B. Wigley -- 1. Introduction -- 2. Cloning -- 3. Case Studies -- 4. Nucleosome Sliding and Histone Exchange Kinetic Assays -- 5. Nucleosome Sliding by Recombinant hINO80 Chromatin Remodeling Complex -- 6. Histone Exchange by Recombinant y SWR1 Chromatin Remodeling Complex -- References -- 3. Analysis of Functional Dynamics of Modular Multidomain Proteins by SAXS and NMR / Walter J. Chazin -- 1. Introduction -- 2. Small-Angle X-Ray Scattering -- 3. Nuclear Magnetic Resonance -- 4. Summary and Conclusion -- Using SAX and NMR to Characterize Architectural Remodeling -- References -- 4. Use of Single-Cysteine Variants for Trapping Transient States in DNA Mismatch Repair / Titia K. Sixma -- 1. Introduction -- 2. Generation of Defined Cysteine Variants -- 3. Cross-linking of Defined Cysteine Variants -- 4. Use of Single-Cysteine Variants to Study Conformational Changes in DNA Mismatch Repair -- 5. Concluding Remarks -- Acknowledgments -- References -- 5. Expression and Structural Analyses of Human DNA Polymerase [MARC+6B] (POLQ) / Sylvie Doublie -- 1. Introduction -- 2. Purification and Structure Determination of Human Pol [MARC+6B] -- 3. Crystallization and Structure Determination of Human Pol [MARC+6B] -- 4. Conclusions -- Acknowledgments -- References -- 6. Structural Studies of RNases H2 as an Example of Crystal Structure Determination of Protein-Nucleic Acid Complexes / Marcin Nowotny -- 1. Introduction -- 2. Purification of RNases H2 -- 3. Biochemical Assays for RNases H2 -- 4. General Considerations in the Crystallization of Protein-Nucleic Acid Complexes -- 5. Crystallization and Structure Determination of RNases H2 -- 6. Concluding Remarks -- Acknowledgment -- References -- 7. DNA-PKcs, Allostery, and DNA Double-Strand Break Repair: Defining the Structure and Setting the Stage / Bancinyane L. Sibanda -- 1. Introduction -- 2. Defining the Structure of DNA-PKcs -- 3. Exploiting Multiple Se-Met Substitutions -- 4. Using Se-Met Sites to Check Sequence Registration -- 5. DNA-PKcs as a Stage for the Assembly of the Actors in NHEJ -- Acknowledgments -- References -- 8. Single-Particle Electron Microscopy Analysis of DNA Repair Complexes / Xiaodong Zhang -- 1. Introduction -- 2. Sample Preparation -- 3. Grid Preparation -- 4. Data Collection -- 5. Data Processing -- Acknowledgments -- References -- 9. Using Atomic Force Microscopy to Characterize the Conformational Properties of Proteins and Protein-DNA Complexes That Carry Out DNA Repair / Dorothy A. Erie -- 1. Introduction -- 2. Methods to Study DNA Repair Complexes With AFM -- 3. Image Analysis -- 4. DREEM Imaging -- 5.Complementary Techniques -- 6. Conclusions -- Acknowledgments -- References -- 10. Single-Molecule Methods for Nucleotide Excision Repair: Building a System to Watch Repair in Real Time / Bennett Van Houten -- 1. Introduction -- 2. Preparation of Defined Lesion Substrates for AFM and DNA Tightrope Assay -- 3. Atomic Force Microscopy -- 4. Single-Molecule DNA Tightrope Assay -- 5. Conclusions -- Acknowledgments -- References -- 11. Next-Generation DNA Curtains for Single-Molecule Studies of Homologous Recombination / Ilya J. Finkelstein -- 1. Introduction -- 2. Methods -- 3. Applications -- 4. Notes -- Acknowledgments -- References -- 12. Detection of Reaction Intermediates in Mg2+-Dependent DNA Synthesis and RNA Degradation by Time-Resolved X-Ray Crystallography / Wei Yang -- 1. Introduction -- 2. DNA Polymerase [MARC+6A] -- 3. Ribonuclease H1 -- 4. Endonuclease V -- 5.X-Ray Data Processing -- 6. Applications and Future Prospects -- Acknowledgments -- References -- 13. Analyzing the Catalytic Activities and Interactions of Eukaryotic Translesion Synthesis Polymerases / M. Todd Washington -- 1. Introduction -- 2. Purification and Structures of Nonclassical Polymerases -- 3. Catalytic Activities of Nonclassical Polymerases -- 4. Interactions of Nonclassical Polymerases -- 5. Conclusions -- Acknowledgments -- References -- 14. Kinetic Methods for Studying DNA Glycosylases Functioning in Base Excision Repair / Alexander C. Drohat -- 1. Introduction -- 2. Kinetic Methods to Study Catalysis by DNA Glycosylases -- 3. Analytical Methods for Monitoring DNA Glycosylase Activity -- 4. Determining Rate Constants From Kinetics Experiments -- Acknowledgment -- References -- 15. Transient Kinetic Methods for Mechanistic Characterization of DNA Binding and Nucleotide Flipping / Patrick J. O'Brien -- 1. Introduction -- 2. General Considerations for Monitoring Binding -- 3. Association Kinetics -- 4.Comparison of Binding and Flipping Mechanisms for AAG and AlkA -- 5. Use of Berkeley Madonna for Evaluating Kinetic Models -- 6. Useful Equations for Kinetic Analysis -- Acknowledgments -- References -- 16. What Combined Measurements From Structures and Imaging Tell Us About DNA Damage Responses / John A. Tainer -- 1. Introduction -- 2. Metabolic Signaling: NAD and DDR by PARP, PARG, and AIF -- 3. DNA Double-Strand Break Responses -- 4. Taking Structural Mechanisms Into Cells by Advanced Imaging -- 5. What's Ahead -- Acknowledgments -- References. | |
| 650 | 0 | |a DNA ligases. | |
| 650 | 0 | |a Cytology. | |
| 650 | 0 | |a Molecular biology. | |
| 650 | 1 | 2 | |a DNA Ligases |x ultrastructure |0 (DNLM)D011088Q000648 |
| 650 | 1 | 2 | |a Molecular Biology |0 (DNLM)D008967 |
| 650 | 2 | |a Cell Biology |0 (DNLM)D003585 | |
| 650 | 2 | |a Cytology |0 (DNLM)D000093222 | |
| 650 | 6 | |a ADN-ligases. |0 (CaQQLa)201-0091992 | |
| 650 | 6 | |a Cytologie. |0 (CaQQLa)201-0040099 | |
| 650 | 6 | |a Biologie mol�eculaire. |0 (CaQQLa)201-0064024 | |
| 650 | 7 | |a molecular biology. |2 aat |0 (CStmoGRI)aat300054470 | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Biochemistry. |2 bisacsh | |
| 650 | 7 | |a Cytology |2 fast |0 (OCoLC)fst00886282 | |
| 650 | 7 | |a DNA ligases |2 fast |0 (OCoLC)fst00886591 | |
| 650 | 7 | |a Molecular biology |2 fast |0 (OCoLC)fst01024734 | |
| 655 | 4 | |a Internet Resources. | |
| 655 | 4 | |a Charts. | |
| 700 | 1 | |a Eichman, Brandt F., |e editor. | |
| 776 | 0 | 8 | |i Print version: |t DNA repair enzymes. |b First edition. |d Cambridge, MA : Academic Press is an imprint of Elsevier, 2017 |z 0128125152 |z 9780128125151 |w (OCoLC)966205098 |
| 830 | 0 | |a Methods in enzymology ; |v v. 592. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780128125151 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/592 |z Texto completo |