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Essentials of noncoding RNA in neuroscience : ontogenetics, plasticity of the vertebrate brain /
Essentials of Noncoding RNA in Neuroscience: Ontogenetics, Plasticity of the Vertebrate Brain focuses on the role of miRNAs in neurogenesis, gliogenesis, neuronal network formation, and the cell biology of forebrain development. The important role miRNAs play in neuronal maturation, neocortex functi...
| Clasificación: | Libro Electrónico |
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| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
London, United Kingdom :
Academic Press, n imprint of Elsevier,
[2017]
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Essentials of Noncoding RNA in Neuroscience; Copyright Page; Contents; List of Contributors; Preface; Acknowledgments; 1 Making and Maintaining MicroRNAs in Animals; Introduction; Genomic Organization; MicroRNA Transcription; Primary MicroRNA Processing; Export From the Nucleus; Pre-MicroRNA Processing; Mature MicroRNA Complex Formation; MicroRNA Stability; Concluding Remarks; Acknowledgements; References; 2 Essentials of miRNA-dependent Control of mRNA Translation and decay, miRNA Targeting Principles, and Methods for Target I ...
- MiRNAs are Posttranscriptional Regulators of Gene ExpressionMiRNA Binding Guides AGO Function to Target RNAs; The Role of the miRNA Seed and the Kinetics of Binding; Alternative 32UTRs Usage and the Molecular Mechanisms of miRNA-Mediated Translational Repression; MiRNAs Buffer Expression Noise to Confer Robustness to Biological Pathways and Act in a Compensatory Manner; Identification of miRNA Targets via Permutation Experiments and Bioinformatics Prediction; Identification of miRNA Sites by Recovering AGO-Bound RNA; Crosslinking and Immunoprecipitation to Uncover Target Sites of RBPs
- Argonaute-Clip Identifies Sites of miRNA Binding but the Targeting Remains UncertainDirect Identification of miRNA:Target Site Interactions by Ligation; Ligases Connect the miRNAs Directly to Their Bound Target Sites Resulting in Chimeric Molecules; Chimeras Recover Endogenous miRNA Interactions, the Majority Possesses Seed Complementarity; Research Opportunities Opened Up by Chimeras: Conservation Analysis of Imperfect Seed Sites and 32 nd Pairing, Classificati ... ; Functionality of Chimera-Identified Interactions; Perspectives; References
- 3 Computational Challenges and -omics Approaches for the Identification of microRNAs and TargetsIntroduction; MiRNA Annotation and Regulation; MiRNA-Transcription Start Sites (TSSs); Databases of miRNA regulation; MiRNA-Gene Interactions; Computational Approaches for the Identification of miRNA-mRNA Predicted Interactions; Databases Cataloging Experimentally Supported miRNA-mRNA-lncRNA Interactions; Investigating miRNA-lncRNA interactions; Functional Analysis Tools and Functional Annotation Databases; MiRNA Functional Analysis and Functional Annotation
- Identification of miRNA-Controlled Pathways and miRNA FunctionsIdentification of Functional miRNAs Using Expression Data; State-of-the-Art Computational-Experimental Approaches; Closing Remarks; References; 4 Methodological Challenges in Functional Investigation and Therapeutic Use of MicroRNAs; RNA Isolation Methods; Analysis of miRNAs Expression; Functional Investigation of miRNAs; Approaches for Loss of miRNA Function; Approaches for Gain-of miRNA Function; Delivery of miRNA Antagonists or Mimics; Viral Vectors; Nonviral carriers; MiRNA:Target Validation; Indirect Validation