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Correlative light and electron microscopy III /
The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam, Netherlands ; Boston, Mass. :
Elsevier / Academic Press,
[2017]
|
| Colección: | Methods in cell biology ;
v. 140. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
MARC
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| 245 | 0 | 0 | |a Correlative light and electron microscopy III / |c edited by Thomas M�uller-Reichert [and] Paul Verkade. |
| 264 | 1 | |a Amsterdam, Netherlands ; |a Boston, Mass. : |b Elsevier / Academic Press, |c [2017] | |
| 264 | 4 | |c �2017 | |
| 300 | |a 1 online resource (xvii, 352 pages) : |b illustrations (some color) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Methods in cell biology, |x 0091-679X ; |v volume 140 | |
| 504 | |a Includes bibliographical references. | ||
| 520 | |a The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field. Includes a brief history and overview on CLEM methods. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Front Cover; Methods in Cell Biology; Series Editors; Methods in CellBiology: Correlative Light and Electron Microscopy III; Copyright; Contents; Contributors; Preface: The Key Lies in the Right Combination; 1 -- Millisecond time resolution correlative light and electron microscopy for dynamic cellular processes; INTRODUCTION; 1. METHODS; 1.1 Chlamydomonas reinhardtii Culture; 1.2 Imaging and Embedding Chamber Preparation; 1.3 Light Microscopy; 1.4 Sample Preparation for Electron Microscopy; 1.4.1 Chemical postfixation and embedding; 1.4.2 Thin sectioning | |
| 505 | 8 | |a 1.4.3 Staining of the sections and application of fiducial markers1.5 Electron Tomography; 1.6 Registration of Light and Electron Microscopy Images; 1.7 Data Analysis; 1.8 Preparation of Flagellar Cross Sections for Correlative Light and Electron Microscopy Analysis; 2. INSTRUMENTATION AND MATERIALS; 2.1 Chlamydomonas reinhardtii culture; 2.2 Imaging and Embedding Chamber Preparation; 2.3 Light Microscopy; 2.4 Sample Preparation for Electron Microscopy; 2.4.1 Chemical postfixation and embedding; 2.4.2 Thin sectioning; 2.4.3 Staining of the sections and application of fiducial markers | |
| 505 | 8 | |a 2.5 Electron Tomography2.6 Registration of Light and Electron Microscopy Images; 2.7 Data Analysis; 2.8 Preparation of Flagellar Cross Sections for Correlative Light and Electron Microscopy Analysis; 3. DISCUSSION AND OUTLOOK; Acknowledgments; REFERENCES; 2 -- 3D subcellular localization with superresolution array tomography on ultrathin sections of various species; INTRODUCTION AND RATIONALE; 1. METHODS-CORE PROTOCOL; 1.1 High-Pressure Freezing; 1.2 Freeze Substitution; 1.3 Embedding; 1.4 Ultramicrotomy; 1.5 Light Microscopy; 1.6 Contrasting and Carbon Coating | |
| 505 | 8 | |a 1.7 Scanning Electron Microscopy1.8 Image Processing and Correlation of Structured Illumination Microscopy and Scanning Electron Microscopy Images; 1.9 Alignment and 3D Reconstruction; 1.9.1 IMOD; 1.9.2 Fiji and AMIRA; 2. METHODS-MODEL-SPECIFIC ADAPTATIONS AND CONSIDERATIONS; 2.1 Caenorhabditis elegans; 2.2 Trypanosoma brucei; 2.3 Social Insects (Apis mellifera and Cataglyphis fortis); 3. INSTRUMENTATION AND MATERIALS; 3.1 High-Pressure Freezing; 3.1.1 General; 3.1.2 Caenorhabditis elegans; 3.1.3 Trypanosoma brucei; 3.1.4 Cataglyphis fortis and Apis mellifera; 3.2 Freeze Substitution | |
| 505 | 8 | |a 3.3 Embedding3.4 Ultramicrotomy; 3.5 Light Microscopy; 3.5.1 Primary Antibodies; 3.6 Contrasting and Carbon Coating; 3.7 Scanning Electron Microscopy; 3.8 Image Processing and Correlation of Structured Illumination Microscopy and Scanning Electron Microscopy Images; 3.9 Alignment and 3D Reconstruction; CONCLUSIONS; Acknowledgments; REFERENCES; 3 -- Preserving the photoswitching ability of standard fluorescent proteins for correlative in-resin super-resolutio ... ; INTRODUCTION; 1. RATIONALE; 2. MATERIALS; 2.1 Instrumentation; 2.2 Materials; 2.3 Chemicals; 3. METHODS; 3.1 Reagent Preparation | |
| 650 | 0 | |a Electron microscopy |x Technique. | |
| 650 | 0 | |a Light. | |
| 650 | 1 | 2 | |a Microscopy, Electron |x methods |0 (DNLM)D008854Q000379 |
| 650 | 2 | 2 | |a Light |0 (DNLM)D008027 |
| 650 | 6 | |a Microscopie �electronique |x Technique. |0 (CaQQLa)201-0067347 | |
| 650 | 6 | |a Lumi�ere. |0 (CaQQLa)201-0005168 | |
| 650 | 7 | |a light (energy) |2 aat |0 (CStmoGRI)aat300056024 | |
| 650 | 7 | |a SCIENCE |x Electron Microscopes & Microscopy. |2 bisacsh | |
| 650 | 7 | |a SCIENCE |x General. |2 bisacsh | |
| 650 | 7 | |a Light |2 fast |0 (OCoLC)fst00998476 | |
| 650 | 7 | |a Electron microscopy |x Technique |2 fast |0 (OCoLC)fst00906689 | |
| 655 | 4 | |a Internet Resources. | |
| 655 | 4 | |a Index not Present. | |
| 655 | 4 | |a Plates. | |
| 700 | 1 | |a M�uller-Reichert, Thomas, |d 1962- |e editor. | |
| 700 | 1 | |a Verkade, Paul, |e editor. | |
| 776 | 0 | 8 | |i Print version: |t Correlative light and electron microscopy. |d Amsterdam, Netherlands ; Boston, Mass. : Elsevier / Academic Press, �2017 |z 9780128099759 |
| 830 | 0 | |a Methods in cell biology ; |v v. 140. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/0091679X/140 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/0091679X |z Texto completo |