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Laboratory methods in microfluidics /
Annotation
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam :
Elsevier,
[2017]
|
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Laboratory Methods in Microfluidics; Copyright Page; Contents; About the Author; Preface; Key features; Acknowledgments; 1 Introduction to Microfluidics; 1.1 Background; 1.1.1 What is Microfluidics?; 1.2 Frequently Used Microfluidic Terms; 1.2.1 Laminar Flow; 1.2.2 Electroosmosis and Electrophoresis; 1.2.3 Separation Resolution; 1.3 Assessment Questions; References; 2 Fabrication of a Glass Microfluidic Device; 2.1 Background; 2.1.1 Photolithographic Procedure; 2.1.2 Chemical Wet Etching; 2.1.3 Drilling Access Holes; 2.1.4 Bonding Two Glass Plates; 2.1.5 Putting Fluid Reservoirs.
- 2.2 Microfluidic Device Design2.3 Chemicals and Supplies; 2.4 Hazards; 2.5 Experimental Procedure; 2.5.1 Photolithography; 2.5.2 Chemical Wet Etching; 2.5.3 Bonding of Two Glass Plates; 2.5.4 Evaluation of Chip; 2.5.5 Lab Report; 2.6 Additional Notes; 2.7 Assessment Questions; References; 3 Fabrication of a Paper Microfluidic Device for Blood-Plasma Separation; 3.1 Background; 3.1.1 Paper Microfluidics; 3.1.2 Protein Assay; 3.2 Microfluidic Device Design; 3.3 Chemicals and Supplies; 3.4 Hazards; 3.5 Experimental Procedure; 3.5.1 Solution Preparation; 3.5.2 Fabrication of the Device.
- 3.5.3 Separation Procedure3.5.4 Lab Report; 3.6 Additional Notes; 3.7 Assessment Questions; References; 4 Fabrication and Testing of a PDMS Microchip; 4.1 Background; 4.2 Design of the Microfluidic Device; 4.3 Chemicals and Supplies; 4.4 Hazards; 4.5 Experimental Procedure; 4.5.1 Fabrication; 4.5.2 Visualization of Laminar Flow; 4.5.3 Lab Report; 4.6 Additional Notes; 4.7 Assessment Questions; References; 5 Determination of Electroosmotic Flow in a Glass Microfluidic Device Using a Neutral Marker; 5.1 Background; 5.2 Microfluidic Device Design; 5.3 Chemicals and Supplies; 5.4 Hazards.
- 5.5 Experimental Procedure5.5.1 Solution Preparation; 5.5.2 Microfluidic Device Preparation; 5.5.3 Data Collection; 5.5.4 Lab Report; 5.6 Additional Notes; References; 6 Electrophoretic Separation in a Microchannel; 6.1 Background; 6.2 Design of the Microfluidic Device; 6.3 Chemicals and Supplies; 6.4 Hazards; 6.5 Experimental Procedure; 6.5.1 Sample Preparation; 6.5.2 Electrophoretic Separation; 6.5.3 Switching Samples in the Microchip; 6.5.4 Data Analysis; 6.5.5 Lab Report; 6.6 Additional Notes; 6.7 Assessment Questions; References.
- 7 A Simple Experiment for the Study of Droplet Microfluidics7.1 Background; 7.2 Microfluidic Device Design; 7.3 Chemicals and Supplies; 7.4 Hazards; 7.5 Experimental Procedure; 7.5.1 Solution Preparation; 7.5.2 Fabrication of Device (Optional); 7.5.3 Droplet Generation; 7.5.4 Image Analysis and Quantitation; 7.5.5 Lab Report; 7.6 Additional Notes; 7.7 Assessment Questions; References; 8 Laminar Flow and Diffusion in a Microchannel; 8.1 Background; 8.2 Microfluidic Device Design; 8.3 Chemicals and Supplies; 8.4 Hazards; 8.5 Experimental Procedure; 8.5.1 Solution Preparation.