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Basic science methods for clinical researchers /

"Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Jalali, Morteza (Editor ), Saldanha, Francesca Y. L. (Editor ), Jalali, Mehdi (Editor ), Davies, S. C. (Sally Claire), 1949- (writer of foreword.)
Formato: Electrónico eBook
Idioma:Inglés
Publicado: London : Academic Press, an imprint of Elsevier, [2017]
Temas:
Acceso en línea:Texto completo
Tabla de Contenidos:
  • The polymerase chain reaction: PCR, qPCR, and RT-PCR / Mehdi Jalali, Justyna Zaborowska, Morteza Jalali
  • Methods of cloning / Alessandro Bertero, Stephanie Brown, Ludovic Vallier
  • Whole-mount in situ hybridization and a genotyping method on single Xenopus embryos / Martyna Lukoseviciute, Robert Lea, Shoko Ishibashi, Enrique Amaya
  • Microarrays: an introduction and guide to their use / Frederick D. Park, Roman Sasik, Tannishtha Reya
  • Analysis of human genetic variations using DNA sequencing / Gregory A. Hawkins
  • Western blot / Tomasz Gwozdz, Karel Dorey
  • The enzyme-linked immunosorbent assay: the application of ELISA in clinical research / Jefte M. Drijvers, Imad M. Awan, Cory A. Perugino, Ian M. Rosenberg, Shiv Pillai
  • Immunofluorescence / Sonali Joshi, Dihua Yu
  • Cell culture: growing cells as model systems in vitro / Charis-P. Segeritz, Ludovic Vallier
  • Flow cytometry / Eleni Chantzoura, Keisuke Kaji
  • Transfection / Semira Sheikh, Amanda S. Coutts, Nicholas B. La Thangue
  • In vivo animal modeling: Drosophila / Michael F. Wangler, Hugo J. Bellen
  • Zebrafish as a research organism: Danio rerio in biomedical research / John Collin, Paul Martin
  • Xenopus as a Model Organism for Biomedical Research / Shoko Ishibashi, Francesca Y.L. Saldanha, Enrique Amaya
  • Basic mouse methods for clinician researchers: harnessing the mouse for biomedical research / Laurens J. Lambert, Mandar D. Muzumdar, William M. Rideout III, Tyler Jacks
  • Appendices. Legal framework on the scientific use of animals in research / David Pettitt, Adam Pettitt, MacKenna Roberts, James Smith, David Brindley
  • Regulatory frameworks for stem cell research / Eleanor Jane Budge, Morteza Jalali
  • Using multiple experimental methods to address basic science research questions / Justyna Zaborowska, Morteza Jalali.