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SCIDIR_ocn973396719 |
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OCoLC |
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20231120112214.0 |
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170222s2017 mau o 000 0 eng d |
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|a Molecular characterization of autophagic responses.
|n Part A /
|c edited by Lorenzo Galluzzi, Jos�e Manuel Bravo-San Pedro and Guido Kroemer.
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264 |
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1 |
|a Cambridge, MA :
|b Academic Press is an imprint of Elsevier,
|c 2017.
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300 |
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|a 1 online resource
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336 |
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|a text
|b txt
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|a computer
|b c
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|a online resource
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|a data file
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|a Methods in enzymology ;
|v volume 587
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505 |
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|a Front Cover; Molecular Characterization of Autophagic Responses, Part A; Copyright; Contents; Contributors; Preface; 1. Introduction; Acknowledgments; References; Chapter One: Correlative Live Cell and Super Resolution Imaging of Autophagosome Formation; 1. Introduction; 2. Live Cell Imaging; 2.1. Brief Overview of Protocol; 3. Correlative Super Resolution Imaging of Autophagosome Formation; 3.1. Fixation of Samples on the Microscope Stage; 3.2. Labeling of Samples; 3.3. Prepare the Microscope for Structured Illumination Imaging; 3.4. Relocate Cell(s) of Interest
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505 |
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|a 3.5. Acquire 3D Structured Illumination Raw Image Data3.6. Reconstruct 3D Structured Illumination Images and Assess for Artifacts; 3.7. Acquire Raw dSTORM Image Data; Acknowledgments; References; Note added in proof; Chapter Two: Quantifying Autophagic Structures in Mammalian Cells Using Confocal Microscopy; 1. Introduction; 2. Detection and Quantification of Autophagic Puncta in Fixed Mammalian Cells; 2.1. Preparation of Coverslips for Confocal Immunofluorescence Microscopy; 2.1.1. Cell Culture and Autophagy Induction; 2.1.2. Permeablization and Antigen Staining; 2.1.3. Direct Fluorescence
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505 |
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|a 2.1.3. Confocal Imaging2.2. Identifying Autophagic Puncta Using Imaris; 2.2.1. Segmenting Cells, Cytosol, and Nuclei Using Imaris; 3. Quantifying Starvation-Induced ATG9 Redistribution by Indirect Immunofluorescence and Confocal Microscopy; 3.1. Background; 3.2. Cell Culture and Indirect Immunofluorescence Labeling; 3.2.1. Cell Culture and Induction of Autophagy; 3.2.2. Indirect Immunofluorescence Labeling; 3.2.3. Confocal Imaging; 3.3. Image Analysis; 4. Quantification of ATG9 Compartment/Autophagosome Contact in Live Cells; 4.1. Preparation of Cell Cultures for Live Cell Confocal Microscopy
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|a 4.2. Analysis of Proximity Between mRFP-ATG9 and GFP-LC3B Structures Using Imaris4.2.1. Manually Creating Cell Surfaces in Imaris; 4.2.2. Measuring Distance Between Two Vesicle Populations Using the Distance Transformation Function; Acknowledgments; References; Chapter Three: The Use of DQ-BSA to Monitor the Turnover of Autophagy-Associated Cargo; 1. Introduction; 2. Materials; 3. Establishment of Polarized Epithelial Cell Cultures; 4. Incorporation of DQ"-BSA Conjugates; 5. Monitoring Autolysosome Formation; 5.1. Rapamycin Stimulation; 5.2. Serum Starvation
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505 |
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|a 5.3. Evaluation of Autolysosome Formation6. Monitoring LC3-Associated Phagolysosome Formation; 7. Immunofluorescence Analysis; 7.1. Labeling Endogenous LC3; 7.2. Confocal Imaging; 7.3. Important Considerations in Experimental Design; 8. Summary; Acknowledgments; References; Chapter Four: Turnover of Lipidated LC3 and Autophagic Cargoes in Mammalian Cells; 1. Introduction; 2. Materials; 2.1. Cell Lines; 2.2. Reagents; 2.3. Antibodies; 2.4. Solutions; 2.5. Equipment; 2.6. Labware; 3. Cell Culture, Treatments, and Sample Collection; 3.1. Cell Culture; 3.2. Treatments; 3.3. Sample Preparation
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588 |
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|a Online resource; title from PDF title page (ScienceDirect, viewed March 3, 2017).
|
650 |
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0 |
|a Cytology.
|
650 |
|
0 |
|a Autophagic vacuoles.
|
650 |
|
0 |
|a Cell death.
|
650 |
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2 |
|a Autophagy
|0 (DNLM)D001343
|
650 |
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2 |
|a Cell Biology
|0 (DNLM)D003585
|
650 |
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2 |
|a Cytology
|0 (DNLM)D000093222
|
650 |
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6 |
|a Cytologie.
|0 (CaQQLa)201-0040099
|
650 |
|
6 |
|a Cellules
|x Mort.
|0 (CaQQLa)201-0110794
|
650 |
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6 |
|a Autophagie (Cytologie)
|0 (CaQQLa)201-0273011
|
650 |
|
7 |
|a SCIENCE
|x Life Sciences
|x Anatomy & Physiology.
|2 bisacsh
|
650 |
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7 |
|a Autophagic vacuoles
|2 fast
|0 (OCoLC)fst00824188
|
650 |
|
7 |
|a Cell death
|2 fast
|0 (OCoLC)fst00850180
|
650 |
|
7 |
|a Cytology
|2 fast
|0 (OCoLC)fst00886282
|
700 |
1 |
|
|a Galluzzi, Lorenzo,
|e editor.
|
700 |
1 |
|
|a Kroemer, Guido,
|e editor.
|
700 |
1 |
|
|a Bravo-San Pedro, Jose Manuel,
|e editor.
|
776 |
0 |
8 |
|i Print version:
|t Molecular characterization of autophagic responses. Part A.
|d Cambridge, MA : Academic Press is an imprint of Elsevier, 2017
|z 0128096756
|z 9780128096758
|w (OCoLC)959872130
|
830 |
|
0 |
|a Methods in enzymology ;
|v v. 587.
|
856 |
4 |
0 |
|u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/587
|z Texto completo
|