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SCIDIR_ocn971134458 |
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170202s2017 maua ob 001 0 eng d |
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|a (OCoLC)971134458
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|a QP551
|b .P76 2017eb
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| 072 |
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|a SCI
|x 007000
|2 bisacsh
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| 082 |
0 |
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|a 572/.6
|2 23
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| 245 |
0 |
0 |
|a Proteomics in Biology.
|n Part B /
|c edited by Arun K. Shukla.
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| 264 |
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1 |
|a Cambridge, MA :
|b Academic Press is an imprint of Elsevier,
|c 2017.
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| 300 |
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|a 1 online resource (xx, 515 pages) :
|b illustrations
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in enzymology ;
|v volume 586
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| 546 |
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|a Text in English.
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| 500 |
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|a Includes indexes.
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| 588 |
0 |
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|a Online resource; title from title details screen (ScienceDirect, viewed February 2, 2017).
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| 505 |
0 |
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|a Front Cover; Proteomics in Biology, Part B; Copyright; Contents; Contributors; Preface; Chapter One: Protein Cysteinyl-S-Nitrosylation: Analysis and Quantification; 1. Introduction; 2. SNOFlo Methodology; 3. Applications; References; Chapter Two: Quantitative Proteomics of the E. coli Membranome; 1. Introduction-Pipeline Overview; 2. Sample Preparation; 2.1. Introduction; 2.2. Preparation of IMVs; 2.3. MS Sample Preparation; 2.3.1. Surface Proteolysis Protocol; 2.3.2. SDS-PAGE and In-Gel Digestion Protocol; 2.3.3. Desalting of Peptides Using C18 Tips.
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| 505 |
8 |
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|a 2.3.4. Additional Proteomics Sample Preparation Methods3. Peptide/Protein Identification; 3.1. Introduction; 3.2. MS Analysis; 3.3. Peptide/Protein Identification; 3.3.1. Peptide Identification-Approach 1; 3.3.2. Peptide Identification-Approach 2; 4. Quantification; 4.1. Introduction; 4.2. Spectral-Based Quantification of Membrane Proteins; 4.3. Intensity-Based Label-Free Quantification of Membrane Proteins; 4.3.1. Peptide Intensity Normalization; 4.3.2. Quantification and Statistical Analysis; 4.3.2.1. Univariate Analysis; 4.3.2.2. Multivariate Analysis; 5. Functional Annotation.
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| 505 |
8 |
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|a 6. ConclusionAcknowledgments; References; Chapter Three: Comparative Analysis and Validation of Different Steps in Glycomics Studies; 1. Introduction; 2. Experimental Design and Randomization; 2.1. Sample Randomization; 3. Sample Preparation for UPLC N-Glycan Analysis; 3.1. IgG Isolation From Plasma/Serum; 3.2. N-Glycan Release; 3.3. Labeling of Free N-Glycans With 2-Aminobenzamide; 3.4. Cleanup of 2-AB-Labeled N-Glycans; 4. Robustness of the Sample Preparation Method; 5. Analysis of Sources of Variation; 6. Method Validation (Between-Day and Between-Analyst Variation).
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| 505 |
8 |
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|a 7. UPLC Analysis of 2-AB-Labeled N-Glycans7.1. Validation of UPLC Method for IgG N-Glycan Analysis; 8. Data Analysis and Quality Control; 9. Summary and Conclusions; Acknowledgments; References; Chapter Four: Analysis of Translocation-Competent Secretory Proteins by HDX-MS; 1. Introduction; 2. Preparation of Polypeptides; 2.1. Isolation of Translocation-Competent and Native Protein States; 2.2. Protein Preparation for HDX; 3. Isotope Labeling; 3.1. Pulsed HDX on Translocation-Competent and Native Protein States; 3.2. Preparation of Unlabeled and Complete Deuteration Controls.
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| 505 |
8 |
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|a 4. Mass Spectrometric Analysis4.1. Online Proteolytic Digestion and LC-MS Analysis; 4.2. Data Analysis; 4.2.1. Peptide Identification; 4.2.2. Deuterium Uptake Determination; 5. Data Visualization and Interpretation; 6. Identifying Statistically Significant Conformational Differences in Comparative Local HDX-MS; 7. Conclusions; Acknowledgments; References; Chapter Five: Site-Specific Quantification of Lysine Acetylation Using Isotopic Labeling; 1. Introduction; 2. Principle of Site-Specific Quantification of Lysine Acetylation Using Isotopic Labeling; 3. Experimental Procedures.
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| 504 |
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|a Includes bibliographical references at the end of each chapters and indexes.
|
| 506 |
1 |
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|a Legal Deposit;
|c Only available on premises controlled by the deposit library and to one user at any one time;
|e The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
|5 WlAbNL
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| 540 |
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|a Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.
|5 WlAbNL
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| 650 |
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0 |
|a Proteomics.
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| 650 |
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2 |
|a Proteomics
|0 (DNLM)D040901
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| 650 |
|
6 |
|a Prot�eomique.
|0 (CaQQLa)201-0363192
|
| 650 |
|
7 |
|a SCIENCE
|x Life Sciences
|x Biochemistry.
|2 bisacsh
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| 650 |
|
7 |
|a Proteomics
|2 fast
|0 (OCoLC)fst01079785
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| 700 |
1 |
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|a Shukla, Arun K.,
|e editor.
|
| 776 |
0 |
8 |
|i Print version:
|t Proteomics in biology. Part B.
|d Cambridge, MA : Academic Press is an imprint of Elsevier, 2017
|z 0128097434
|z 9780128097434
|w (OCoLC)959034752
|
| 830 |
|
0 |
|a Methods in enzymology ;
|v v. 586.
|
| 856 |
4 |
0 |
|u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/586
|z Texto completo
|
