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Applications of Mass Spectrometry Imaging to Cancer /
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Cambridge, MA :
Academic Press is an imprint of Elsevier,
2017.
|
| Colección: | Advances in cancer research ;
v. 134. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Applications of Mass Spectrometry Imaging to Cancer; Copyright; Contents; Contributors; Preface; References; Chapter One: The Importance of Histology and Pathology in Mass Spectrometry Imaging; 1. Importance of Pathology; 2. Possible Errors Caused by Tissue Inherent Factors-Why Histology Is Important for Supervised Analysis; 3. Possible Errors Caused by Sample Inherent Factors-Small Pretherapeutic Biopsies; 4. Possible Errors Caused by Sample Preparations-Artifacts Are Not Your Friend.
- 5. Possible Errors Caused by Ill-Defined Sample Groups in the Training Set-Keep It Black and White/Shades of Gray Are Not ... 6. Conclusion; References; Chapter Two: Applications of Mass Spectrometry Imaging to Cancer; 1. Introduction; 1.1. The Advantages of MSI; 1.2. The Basic Principles of MSI; 1.2.1. MALDI-TOF; 1.2.2. MALDI-FT-ICR; 1.2.3. SIMS-TOF; 1.2.4. DESI; 2. Protein MSI in Cancer Research; 2.1. Distinguishing Tissue Types by Peptide MSI; 2.2. Determining Tumor Margins by Peptide MSI; 2.3. Prediction of Metastasis by Peptide MSI; 2.4. Analysing Chemoresponse by Peptide MSI.
- 2.5. Identification of Diagnostic and Prognostic Markers by Peptide MSI2.6. Characterisation of Intra- and Intertumor Variability by Peptide MSI; 2.7. Practical Considerations for Proteolytic Peptide MSI: Sample Preparation; 2.8. Practical Considerations for Peptide MSI: Spatial Resolution; 2.9. Practical Considerations for Peptide MSI: Mass Analysers; 2.10. Practical Considerations for Peptide MSI: Identification; 3. Lipid MSI in Cancer Research; 3.1. Profiling Lipids in Cancer by DESI-MSI; 3.2. Profiling Lipids in Cancer by SIMS-MSI; 4. Glycan MSI in Cancer Research.
- 5. Drug Imaging in Cancer Research5.1. MALDI-MSI on Tissue Sections; 5.2. MALDI-MSI on Whole Body Sections; 5.3. MALDI-MSI on 3D Tissue Cultures; 6. Data Analysis; 6.1. Spatial Information; 6.2. Preprocessing: Peak Detection; 6.3. Classification of FFPE-TMAs and the Importance of Dimension Reduction; 7. Concluding Remarks; 7.1. The Future of MSI: Molecular Pathology; References; Chapter Three: Assessing the Potential of Metal-Assisted Imaging Mass Spectrometry in Cancer Research; 1. Introduction; 2. Material; 3. Silver-Assisted IMS; 3.1. Preparation of Silver-Coated Glass Slides.
- 3.2. Tissue Deposition on Silver- or ITO-Coated Slides3.3. Silver Deposition on Tissue Section; 3.4. LDI Data Acquisition of a Silver-Coated Tissue Section; 3.5. Fast Optimization of New Tissue Sections; 4. Gold-Assisted IMS; 4.1. Preparation of Gold-Coated Glass Slides; 4.2. Tissue Deposition on ITO- or Gold-Coated Slides; 4.3. Sodium Salt Deposition; 4.4. Gold Deposition on Tissue Section; 4.5. LDI Data Acquisition of a CBS-Au-Coated Tissue Section; 5. Applications to Cancer Research; 6. Concluding Remarks; References.