PCR guru : an ultimate benchtop reference for molecular biologists /

"PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of emplo...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autores principales: Najafov, Ayaz (Autor), Hoxhaj, Gerta (Autor)
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam : Academic Press, Elsevier, [2017]
Temas:
Polymerase chain reaction.
Molecular biology.
Polymerase Chain Reaction > methods
Polymerase Chain Reaction
Molecular Biology
R�eaction en cha�ine de la polym�erase.
Biologie mol�eculaire.
molecular biology.
SCIENCE > Life Sciences > Biochemistry.
Molecular biology
Polymerase chain reaction
Electronic books.
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Cover; Title Page; Copyright Page; Dedication; Contents; Biography; Preface; Acknowledgments; Note to the Reader; Chapter 1
  • Introduction; Abstract; 1.1
  • A bit of history; 1.2
  • What is PCR?; 1.3
  • PCR components; 1.4
  • Exponential nature of PCR; 1.5
  • Popularity of PCR; Chapter 2
  • Procedure; Abstract; 2.1
  • Primer design; 2.2
  • Standard PCR setup; 2.3
  • Controls; 2.4
  • Cycling parameters; 2.4.1 Denaturation; 2.4.2 Annealing; 2.4.3 Extension; Chapter 3
  • Good PCR Practices; Abstract; Chapter 4
  • Optimization and Troubleshooting; Abstract; 4.1
  • Optimization rules.
  • 4.2
  • General optimization strategies4.2.1
  • Annealing Temperature Gradient; 4.2.2
  • Mg++ Titration; 4.2.3
  • Hot Start; 4.2.4
  • Touchdown PCR; 4.2.5
  • Additives; 4.3
  • Troubleshooting specific problems; 4.3.1
  • Problem Type 1; 4.3.2
  • Problem Type 2; 4.3.3
  • Problem Type 3; 4.3.4
  • Problem Type 4; 4.3.5
  • Problem Type 5; 4.3.6
  • Other Problem Types; Chapter 5
  • Tips and Tricks; Abstract; Chapter 6
  • Special Cases; Abstract; Glossary and Abbreviations; A; B; C; D; E; G; K; M; N; O; P; Q; R; S; T; X; Appendix A
  • Agarose Gel Electrophoresis Protocol; Appendix B
  • Silver Staining of DNA Gels.
  • Appendix C
  • Plasmid Miniprep Protocol (by using "QIAprep Spin Miniprep" Kit)Appendix D
  • Restriction Enzyme Digestion Protocol; Appendix E
  • Protocol for DNA Concentration Estimation Using Hoechst 33258; Appendix F
  • Formulas; Appendix G
  • Common Primers for Sequencing, Promoters, Housekeeping Genes, and Reporters; Appendix H
  • Tags; Appendix I
  • Thermostable DNA polymerases; Appendix J
  • Example of a Daily PCR Log; Appendix K
  • Common Buffers and Solutions; Appendix L
  • Useful Numbers; Appendix M
  • Yeast Colony PCR; Useful Websites; References; Index; Back Cover.

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