Mass spectrometry for the clinical laboratory.

Mass Spectrometry for the Clinical Laboratory is an accessible guide to mass spectrometry and the development, validation, and implementation of the most common assays seen in clinical labs. It provides readers with practical examples for assay development, and experimental design for validation to...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Nair, Hari, Clarke, William, 1970-
Formato: Electrónico eBook
Idioma:Inglés
Publicado: [Amsterdam] : Elsevier Ltd. : Academic Press, [2017]
Temas:
Mass spectrometry.
Diagnosis, Laboratory.
Medical laboratory technology.
Mass Spectrometry
Clinical Laboratory Techniques
Spectrom�etrie de masse.
Diagnostics biologiques.
M�edecine > Laboratoires > Technique.
mass spectrometry.
SCIENCE > Chemistry > Analytic.
Medical laboratory technology
Diagnosis, Laboratory
Mass spectrometry
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Cover ; Title page; Copyright page; Contents; List of Contributors; Preface; Chapter 1
  • Mass spectrometry in the clinical laboratory: determining the need and avoiding pitfalls; 1
  • Clinical mass spectrometry; 1.1
  • Basic mass spectrometry concepts; 1.2
  • Common ion sources for clinical mass spectrometry; 1.2.1
  • Electrospray Ionization (ESI); 1.2.2
  • Atmospheric Pressure Chemical Ionization (APCI); 1.2.3
  • Atmospheric Pressure Photoionization (APPI); 1.3
  • Commonly used mass analyzers; 1.3.1
  • Quadrupole Mass Analyzers; 1.3.2
  • Time-of-Flight Mass Analyzers; 1.3.3
  • Ion Trap Mass Analyzers.
  • 1.3.4
  • Hybrid Tandem Mass Analyzers1.4
  • Determining the need for clinical LC-MS; 1.5
  • Potential pitfalls and addressing the challenges; 1.5.1
  • External Quality Assessment and Quality Control; 1.5.2
  • Matrix Effects and Interferences; 1.5.3
  • Technical Hurdles; 1.5.4
  • Practical Considerations; References; Chapter 2
  • Application specific implementation of mass spectrometry platform in clinical laboratories; 1
  • Mass spectrometry versus automated immunoassay platforms; 2
  • Early decisions in the implementation of mass spectrometry in the laboratory.
  • 3
  • Importance of internal standards in mass spectrometry4
  • Matrix effects, extraction efficiency, and ion suppression/enhancement; 5
  • Analyte extraction; 6
  • Choice of separation technique: GC versus LC; 7
  • Choice of stationary phase; 8
  • Choice of ionization techniques; 9
  • Choice of mass analyzer; 10
  • Summary; References; Chapter 3
  • Sample preparation techniques for mass spectrometry in the clinical laboratory; 1
  • Why is sample preparation needed for LC-MS/MS?; 2
  • Selecting a sample preparation protocol; 3
  • Description of commonly used clinical sample preparation protocols.
  • 3.1
  • Simple-dilution sample preparation protocol (DIL)3.2
  • Simple-protein precipitation sample preparation protocol (PPT); 3.3
  • More complex-liquid-liquid extraction sample preparation protocol (LLE); 3.4
  • Commercial media-phospholipid removal sample preparation protocol (PLR); 3.5
  • Commercial media-supported liquid extraction sample preparation protocol (SLE or SALL); 3.6
  • Commercial media-solid phase extraction sample preparation protocol (SPE); 3.7
  • Online SPE; 4
  • Evaluation of sample preparation protocols; 4.1
  • Evaluating chromatography.
  • 4.2
  • Evaluating signal to noise (S/N) at the LLOQ4.3
  • Evaluating matrix effect (ME); 4.3.1
  • Quantitative Matrix Effect Experiment; 4.3.2
  • Qualitative Postcolumn Matrix Effect Experiment; 4.3.3
  • Phospholipid Direct Detection Experiment; 4.3.4
  • Matrix Mixing Experiment; 4.4
  • Evaluating method performance; 4.5
  • Evaluating practicality; 4.6
  • Evaluating robustness; 5
  • Comparison of sample preparation protocols; 6
  • Optimization of sample preparation protocols; 6.1
  • Generic-optimizing for 96-well format; 6.2
  • Generic-optimizing LC injection solvent composition.
  • 6.2.1
  • Analyte Solubility.

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