Single-molecule enzymology : fluorescence-based and high-throughput methods /

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Spies, Maria (Editor ), Chemla, Yann R. (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Cambridge, MA : Elsevier : Academic Press, 2016.
Colección:Methods in enzymology ; v. 581.
Temas:
Fluorescence spectroscopy > Congresses.
Fluorescent probes > Congresses.
Luminescence spectroscopy > Congresses.
Luminescent probes > Congresses.
Fluorescence spectroscopy.
Fluorescence microscopy.
Spectrometry, Fluorescence
Microscopy, Fluorescence
Spectroscopie de fluorescence > Congr�es.
Sondes fluorescentes > Congr�es.
Spectroscopie de luminescence > Congr�es.
Sondes luminescentes > Congr�es.
Spectroscopie de fluorescence.
Microscopie de fluorescence.
fluorescence microscopy.
SCIENCE > Chemistry > Analytic.
Fluorescence microscopy
Fluorescence spectroscopy
Fluorescent probes
Luminescence spectroscopy
Luminescent probes
Congress
proceedings (reports)
Conference papers and proceedings
Conference papers and proceedings.
Actes de congr�es.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Chapter 1. Direct fluorescent imaging of translocation and unwinding by individual DNA helicases
  • Chapter 2. Single-molecule imaging with one color fluorescence
  • Chapter 3. Measuring membrane protein dimerization equilibrium in lipid bilayers by single-molecule fluorescence microsopy
  • Chapter 4. Fluorescent labeling of proteins in whole cell extracts for single-molecule imaging
  • Chapter 5. Quantifying the assembly of multicomponent molecular machines by single-molecule total internal reflection fluorescence microscopy
  • Chapter 6. How to measure separations and angles between intramolecular fluorescent markers
  • Chapter 7. Precisely and accurately inferring single-molecule rate constants
  • Chapter 8. Quantification of functional dynamics of membrane proteins reconstituted in nanodiscs membranes by single turnover functional readout
  • Chapter 9. Putting Humpty-Dumpty together: clustering the functional dynamics of single biomolecular machines such as the splicosome
  • Chapter 10. Single-molecule FRET to measure conformational dynamics of DNA mismatch repair proteins
  • Chapter 11. Single-molecule confocal FRET microscopy to dissect conformational changes in the catalytic cycle of DNA topoisomerases
  • Chapter 12. Probing the conformational landscape of DNA polymerases using diffusion-based single-molecule FRET
  • Chapter 13. Methods for investigating DNA accessibility with single nucleosomes
  • Chapter 14. Single-molecule fluorescence studies of fast protein folding
  • Chapter 15. Single-molecule multicolor FRET assay for studying structural dynamics of biomolecules
  • Chapter 16. A multicolor single-molecule FRET approach to study protein dynamics and interactions simultaneously
  • Chapter 17. Interferometric scattering microscopy for the study of molecular motors
  • Chapter 18. Enzyme kinetics in femtoliter arrays
  • Author index
  • Subject index.

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