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016 7 |a 018105549  |2 Uk 
019 |a 962065471  |a 1052766306  |a 1110388926 
020 |a 9780128095478  |q (electronic bk.) 
020 |a 0128095474  |q (electronic bk.) 
020 |z 9780128092675 
020 |z 012809267X 
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072 7 |a SCI  |x 013010  |2 bisacsh 
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082 0 4 |a 543/.56  |2 23 
245 0 0 |a Single-molecule enzymology :  |b fluorescence-based and high-throughput methods /  |c edited by Maria Spies and Yann R. Chemla. 
264 1 |a Cambridge, MA :  |b Elsevier :  |b Academic Press,  |c 2016. 
300 |a 1 online resource (xviii, 598 pages) :  |b illustrations (chiefly color) 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
347 |a data file  |2 rda 
490 1 |a Methods in enzymology,  |x 0076-6879 ;  |v volume 581 
504 |a Includes bibliographical references and indexes. 
588 0 |a Online resource; title from HTML title page (ScienceDirect, viewed November 2, 2016). 
505 0 |a Chapter 1. Direct fluorescent imaging of translocation and unwinding by individual DNA helicases -- Chapter 2. Single-molecule imaging with one color fluorescence -- Chapter 3. Measuring membrane protein dimerization equilibrium in lipid bilayers by single-molecule fluorescence microsopy -- Chapter 4. Fluorescent labeling of proteins in whole cell extracts for single-molecule imaging -- Chapter 5. Quantifying the assembly of multicomponent molecular machines by single-molecule total internal reflection fluorescence microscopy -- Chapter 6. How to measure separations and angles between intramolecular fluorescent markers -- Chapter 7. Precisely and accurately inferring single-molecule rate constants -- Chapter 8. Quantification of functional dynamics of membrane proteins reconstituted in nanodiscs membranes by single turnover functional readout -- Chapter 9. Putting Humpty-Dumpty together: clustering the functional dynamics of single biomolecular machines such as the splicosome -- Chapter 10. Single-molecule FRET to measure conformational dynamics of DNA mismatch repair proteins -- Chapter 11. Single-molecule confocal FRET microscopy to dissect conformational changes in the catalytic cycle of DNA topoisomerases -- Chapter 12. Probing the conformational landscape of DNA polymerases using diffusion-based single-molecule FRET -- Chapter 13. Methods for investigating DNA accessibility with single nucleosomes -- Chapter 14. Single-molecule fluorescence studies of fast protein folding -- Chapter 15. Single-molecule multicolor FRET assay for studying structural dynamics of biomolecules -- Chapter 16. A multicolor single-molecule FRET approach to study protein dynamics and interactions simultaneously -- Chapter 17. Interferometric scattering microscopy for the study of molecular motors -- Chapter 18. Enzyme kinetics in femtoliter arrays -- Author index -- Subject index. 
650 0 |a Fluorescence spectroscopy  |v Congresses. 
650 0 |a Fluorescent probes  |v Congresses. 
650 0 |a Luminescence spectroscopy  |v Congresses. 
650 0 |a Luminescent probes  |v Congresses. 
650 0 |a Fluorescence spectroscopy. 
650 0 |a Fluorescence microscopy. 
650 2 |a Spectrometry, Fluorescence  |0 (DNLM)D013050 
650 2 |a Microscopy, Fluorescence  |0 (DNLM)D008856 
650 6 |a Spectroscopie de fluorescence  |0 (CaQQLa)201-0066421  |v Congr�es.  |0 (CaQQLa)201-0378219 
650 6 |a Sondes fluorescentes  |0 (CaQQLa)201-0250401  |v Congr�es.  |0 (CaQQLa)201-0378219 
650 6 |a Spectroscopie de luminescence  |0 (CaQQLa)201-0044100  |v Congr�es.  |0 (CaQQLa)201-0378219 
650 6 |a Sondes luminescentes  |0 (CaQQLa)000259995  |v Congr�es.  |0 (CaQQLa)201-0378219 
650 6 |a Spectroscopie de fluorescence.  |0 (CaQQLa)201-0066421 
650 6 |a Microscopie de fluorescence.  |0 (CaQQLa)201-0014904 
650 7 |a fluorescence microscopy.  |2 aat  |0 (CStmoGRI)aat300252489 
650 7 |a SCIENCE  |x Chemistry  |x Analytic.  |2 bisacsh 
650 7 |a Fluorescence microscopy  |2 fast  |0 (OCoLC)fst00928103 
650 7 |a Fluorescence spectroscopy  |2 fast  |0 (OCoLC)fst00928106 
650 7 |a Fluorescent probes  |2 fast  |0 (OCoLC)fst00928131 
650 7 |a Luminescence spectroscopy  |2 fast  |0 (OCoLC)fst01003662 
650 7 |a Luminescent probes  |2 fast  |0 (OCoLC)fst01003664 
655 2 |a Congress  |0 (DNLM)D016423 
655 7 |a proceedings (reports)  |2 aat  |0 (CStmoGRI)aatgf300027316 
655 7 |a Conference papers and proceedings  |2 fast  |0 (OCoLC)fst01423772 
655 7 |a Conference papers and proceedings.  |2 lcgft 
655 7 |a Actes de congr�es.  |2 rvmgf  |0 (CaQQLa)RVMGF-000001049 
700 1 |a Spies, Maria,  |e editor. 
700 1 |a Chemla, Yann R.,  |e editor. 
830 0 |a Methods in enzymology ;  |v v. 581. 
856 4 0 |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/581  |z Texto completo