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Methods for analysis of carbohydrate metabolism in photosynthetic organisms : plants, green algae and cyanobacteria /
Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms: Plants, Green Algae and Cyanobacteria examines both general and detailed aspects of carbohydrate metabolism in photosynthetic organisms, along with the four main oligosaccharides and each enzymatic reaction that gives birth...
| Clasificación: | Libro Electrónico |
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| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
London :
Academic Press,
2016, �2017.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms; Copyright Page; Dedication; Contents; Biography; Preface; I: General Analytical Methods; 1 Determination of Carbohydrates Metabolism Molecules; 1.1 Introduction; 1.2 Determination of Reducing Sugars by the Somogyi-Nelson Method; 1.3 Determination of Total Sugars by the Anthrone Method; 1.4 Determination of Total Sugars by the Phenol-Sulfuric Acid Assay; 1.5 Enzymatic Determination of Glucose and Fructose; 1.6 Enzymatic Determination of Sucrose.
- 1.6.1 Spectrophotometric Method (Measurement of NADPH Absorption)1.6.2 Direct Fluorometric Method (Measurement by NADPH Native Fluorescence); 1.6.3 Assay With Fluorescence Enhancement; 1.7 Determination of Glucose and Galactose by an Enzymatic Colorimetric Method; 1.8 Determination of Glucose by an Enzymatic Fluorometric Method; 1.9 Determination of Amino Sugars by the Elson-Morgan Method; 1.10 Determination of Acetyl Amino Sugars by the Morgan-Elson Method; 1.11 Determination of Uronic Acids by the Carbazole Assay; 1.12 Determination of Pentoses by the Orcinol Procedure.
- 1.13 Determination of Fructose Derivatives by the Thiobarbituric Acid Assay1.14 Determination of Inorganic Phosphate by the Fiske-Subbarow Assay; 1.15 Determination of Inorganic Phosphate by the Chifflet's Method; 1.16 Determination of Uridine Diphosphate by the Measurement of Pyruvate; 1.16.1 Colorimetric Method Using 2,4-Dinitrophenylhydrazine; 1.16.2 Spectrophotometric Method Using Lactic Dehydrogenase; 1.17 Determination of UDP-Glucose Using UDP-Glucose Dehydrogenase; 1.18 Determination of UDP-Glucose Using UDP-Glucose Pyrophosphorylase; Further Reading and References.
- II: Purification and Analysis of Proteins and Carbohydrates2 Preparation of Protein Extracts; 2.1 Introduction; 2.2 Extraction of Proteins; 2.2.1 Source Material; 2.2.2 Homogenization Buffer Composition; 2.2.3 Breakdown of Biological Material (Cells and Tissues Disruption); 2.2.4 Cell Permeabilization; 2.3 Laboratory Procedures for Protein Extraction; 2.3.1 Preparation of Acetone Powder from Wheat Leaves (Calder�on and Pontis, 1985); 2.3.2 Preparation of Plant Tissue Extracts; 2.3.2.1 Preparation of leaf or seedling extracts.
- 2.3.2.2 Preparation of protein extracts from spinach and Arabidopsis chloroplasts2.3.3 Preparation of Extracts from Photosynthetic Microorganisms; 2.3.3.1 Chlorella vulgaris; 2.3.3.2 Chlamydomonas reinhardtii; 2.3.3.3 Ostreococcus tauri; 2.3.3.4 Cyanobacteria; 2.4 Protein Extract Desalting; Further Reading and References; 3 Protein and Carbohydrate Separation and Purification; 3.1 Introduction; 3.2 Enzymatic Protein Purification; 3.2.1 Methods Based on Protein Solubility; 3.2.2 Protein Fractionation by Batch Adsorption; 3.2.3 Protein Fractionation by Chromatographic Techniques.