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|a QP552.S37
|b S47 2016eb
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|b .M47 v.136eb
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|2 bisacsh
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| 082 |
0 |
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|a 572/.6
|2 23
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| 245 |
0 |
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|a Septins /
|c edited by Amy S. Gladfelter.
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| 250 |
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|a First edition.
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| 264 |
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1 |
|a Amsterdam :
|b Academic Press is an imprint of Elsevier,
|c 2016.
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| 300 |
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|a 1 online resource (xv, 366 pages) :
|b illustrations.
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| 336 |
|
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in cell biology,
|x 0091-679X ;
|v v. 136
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| 546 |
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|a Text in English.
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| 504 |
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|a Includes bibliographical references and index.
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0 |
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|a Online resource; title from PDF title page (ScienceDirect, viewed on July 28, 2016).
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0 |
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|a Front Cover; Methods in Cell Biology: Septins ; Series Editors; Methods in Cell Biology: Septins ; Copyright; Contents; Contributors; Preface; 1 -- The nonopisthokont septins: how many there are, how little we know about them, and how we might learn more ; INTRODUCTION; 1. THE WIDE PHYLOGENETIC DISTRIBUTION AND INFERRED ANCIENT ORIGIN OF THE SEPTINS; 1.1 Search Methods; 1.2 Apparent Absence of Septins From the Amoebozoa; 1.3 Presence of Septins in Some, but Not All, Planta Lineages; 1.4 Presence of Septins in Cryptophytes and Haptophytes
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8 |
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|a 1.5 Presence of Septins in At Least One Rhizarian Lineage1.6 Presence of Septins in At Least Some Alveolate Lineages; 1.7 Presence of Septins in At Least Some Heterokont Lineages; 1.8 Apparent Absence of Septins From the Excavata; 1.9 General Conclusions; 2. THE NEARLY TOTAL LACK OF FUNCTIONAL INFORMATION ABOUT THE NONOPISTHOKONT SEPTINS; 3. THE PROSPECTS FOR INSIGHTS FROM FUNCTIONAL ANALYSES OF NONOPISTHOKONT SEPTINS; Acknowledgments; REFERENCES; 2 -- Preparing recombinant yeast septins and their analysis by electron microscopy ; INTRODUCTION; 1. METHODS
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| 505 |
8 |
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|a 1.1 Preparation of Recombinant Budding Yeast Septin Complexes2. MATERIALS; 2.1 Sample Preparation for Electron Microscopy; 2.1.1 Sample preparation for negative stain samples; 2.1.2 Sample preparation for thin film frozen hydrated samples; 3. MATERIALS; 3.1 Microscopy and Image Two-Dimensional Analysis; 4. MATERIALS; 4.1 Tagging Strategies to Label Septin Subunits Within Complexes; 4.1.1 Antibody labeling; 4.1.2 Covalent bound tags; CONCLUSION; Acknowledgments; REFERENCES; 3 -- A FRET-based method for monitoring septin polymerization and binding of septin-associated proteins ; INTRODUCTION
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| 505 |
8 |
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|a 1. PROTEIN PREPARATION AND LABELING1.1 Expression and Purification of Single-Cys Yeast Septin Heterooctamers; 1.2 Single-Cys Septin Subunits and Dye Labeling by Maleimide Chemistry; 1.3 Selection of Fluorophores for F�orster Resonance Energy Transfer Analysis; 2. DATA COLLECTION AND ANALYSIS; 2.1 Spectroscopy Data Collection; 2.2 Semiquantitative Sensitized Emission; 2.3 Principal Component Analysis; CONCLUSIONS AND OUTLOOK; Acknowledgments; REFERENCES; 4 -- In vitro reconstitution of septin assemblies on supported lipid bilayers ; INTRODUCTION; 1. RATIONAL
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| 505 |
8 |
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|a 1.1 TIRF-Based Assays for Visualizing Cytoskeletal Properties1.2 Supported Lipid Bilayers; 2. CLEANING OF GLASS COVERSLIPS; 2.1 Rational; 2.2 Reagents and Equipment; 2.3 Protocol for RCA Cleaning of Glass Coverslips; 3. PREPARATION OF SMALL UNILAMELLAR VESICLES; 3.1 Rational; 3.2 Reagents and Equipment; 3.3 Protocol for the Preparation of Small Unilamellar Vesicles; 4. FORMATION OF SUPPORTED LIPID BILAYERS; 4.1 Reagents and Equipment; 4.2 Fusion Protocol; 5. VISUALIZING SEPTIN ADSORPTION AND POLYMERIZATION ON SUPPORTED LIPID BILAYERS; CONCLUDING REMARKS; REFERENCES
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|a 5 -- Visualization of in vivo septin ultrastructures by platinum replica electron microscopy
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0 |
|a Septins.
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| 650 |
1 |
2 |
|a Septins
|0 (DNLM)D058112
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| 650 |
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6 |
|a Septines.
|0 (CaQQLa)000266418
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| 650 |
|
7 |
|a SCIENCE
|x Life Sciences
|x Biochemistry.
|2 bisacsh
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| 650 |
|
7 |
|a Septins
|2 fast
|0 (OCoLC)fst01745707
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| 655 |
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0 |
|a Electronic book.
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| 655 |
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4 |
|a Internet Resources.
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| 700 |
1 |
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|a Gladfelter, Amy S.,
|d 1974-
|e editor.
|
| 730 |
0 |
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|a Elsevier life sciences books series.
|5 CaBVaS
|
| 776 |
0 |
8 |
|i Print version:
|z 9780128039984
|z 0128039981
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| 830 |
|
0 |
|a Methods in cell biology ;
|v v. 136.
|
| 856 |
4 |
0 |
|u https://sciencedirect.uam.elogim.com/science/bookseries/0091679X/136
|z Texto completo
|
| 856 |
4 |
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|u https://sciencedirect.uam.elogim.com/science/bookseries/0091679X/136
|z Texto completo
|
