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Enzymes of epigenetics. Part A /

Enzymes of Epigenetics, one of two new volumes in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods that are employed to study epigenetic regulation and includes structural, bioche...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Marmorstein, Ronen (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Cambridge, MA : Academic Press is an imprint of Elsevier, 2016.
Colección:Methods in enzymology ; v. 573.
Temas:
Acceso en línea:Texto completo
Tabla de Contenidos:
  • In vitro chromatin assembly : strategies and quality control / Uma Muthurajan, Francesca Mattiroli, Serge Bergeron, Keda Zhou, Yajie Gu, Srinivas Chakravarthy, Pamela Dyer, Thomas Irving and Karolin Luger
  • Preparation, crystallization, and structure determination of chromatin enzyme/nucleosome complexes / Robert K. McGinty, Ravindra D. Makde and Song Tan
  • Preparation of recombinant centromeric nucleosomes and formation of complexes with nonhistone centromere proteins / Nikolina Sekulic and Ben E. Black
  • Functional characterization of histone chaperones using SNAP-tag-based imaging to assess De Novo histone deposition / Camille Cl�ement, Isabelle Vassias, Dominique Ray-Gallet and Genevi�eve Almouzni
  • Analysis of nucleosome sliding by ATP-dependent chromatin remodelling enzymes / Coral Y. Zhou and Geeta J. Narlikar
  • In vitro activity assays for MYST histone acetyltransferases and adaptation for high-throughput inhibitor screening / Cheryl E. McCullough and Ronen Marmorstein
  • Preparation and biochemical analysis of classical histone deacetylases / Alejandro Villagra, Eva Sahakian and Edward Seto
  • Recombinant preparation, biochemical analysis, and structure determination of sirtuin family histone/protein deacylases / Benjamin Suenkel and Clemens Steegborn
  • Preparation, biochemical analysis, and structure determination of SET domain histone methyltransferases / Elisa Bergamin and Jean Francois Couture
  • Expression, purification, and biochemical analysis of the LSD1/KDM1A histone demethylase / Benoit Laurent and Yang Shi
  • LSD1 histone demethylase assays and inhibition / Dawn Hayward and Philip A. Cole
  • Purification, biochemical analysis, and structure determination of JmjC lysine demethylases / Swathi Krishnan and Raymond C. Trievel
  • Preparation and analysis of native chromatin-modifying complexes / Yannick Doyon and Jacques C�ot�e
  • Preparation, biochemical analysis, and structure determination of the bromodomain, an acetyl-lysine binding domain / Chunyan Ren, Lei Zeng and Ming-Ming Zhou
  • Preparation, biochemical analysis, and structure determination of methyllysine readers / Catherine A. Musselman and Tatiana G. Kutateladze
  • Quantification of oxidized 5-methylcytosine bases and TET enzyme activity / Monica Yun Liu, Jamie E. DeNizio and Rahul M. Kohli
  • Characterization of how DNA modifications affect DNA binding by C2H2 zinc finger proteins / Anamika Patel, Hideharu Hashimoto, Xing Zhang and Xiaodong Cheng
  • Crystallographic studies of telomerase / Hunter Hoffman and Emmanuel Skordalakes
  • Detection and analysis of long noncoding RNAs / Fan Lai, Ezra Blumenthal and Ramin Shiekhattar
  • Identifying centromeric RNAs involved in histone dynamics in vivo / Delphine Qu�enet, David Sturgill and Yamini Dalal.