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G protein-coupled receptors : signaling, trafficking and regulation /
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Cambridge, MA, USA :
Academic Press is an imprint of Elsevier,
2016.
|
| Edición: | First edition. |
| Colección: | Methods in cell biology ;
v. 132. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo Texto completo |
MARC
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| 245 | 0 | 0 | |a G protein-coupled receptors : |b signaling, trafficking and regulation / |c edited by Arun K. Shukla. |
| 250 | |a First edition. | ||
| 264 | 1 | |a Cambridge, MA, USA : |b Academic Press is an imprint of Elsevier, |c 2016. | |
| 300 | |a 1 online resource (480 pages) : |b color illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Methods in cell biology ; |v volume 132 | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | |a Front Cover; Series Editors; Methods in Cell Biology G Protein-Coupled Receptors: Signaling, Trafficking and Regulation; Copyright; Contents; Contributors; Preface; 01 -- Trafficking, Localization and Imaging of GPCRs; 1 -- Localization and signaling of GPCRs in lipid rafts; INTRODUCTION; 1. LOCALIZATION OF GPCRS IN LIPID RAFTS; 1.1 Isolation of Lipid Rafts; 1.1.1 Detergent-free method; 1.1.2 Detergent-based method; 1.1.3 Immunoblotting and data interpretation; 1.2 Localization of GPCRs in Lipid Rafts; 1.2.1 Cells in suspension; 1.2.2 Adherent cells; 2. GPCR SIGNALING IN LIPID RAFTS | |
| 505 | 8 | |a 2.1 Perturbation of Raft Stability2.2 Changing the Cholesterol Content; 2.3 Fluorescence Imaging; REFERENCES; 2 -- Imaging GPCRs trafficking and signaling with total internal reflection fluorescence microscopy in cultured neurons; 1. IMAGE ACQUISITION; 1.1 Important Considerations before Imaging; 1.2 Materials; 1.2.1 Cell culture; 1.2.2 TIRF microscopy equipment and settings; 1.3 Imaging; 1.3.1 Cell culture; 1.3.2 Live cell imaging; 1.4 Notes; 2. IMAGE ANALYSIS; 3. FINAL COMMENTS; Acknowledgments; REFERENCES; 3 -- Trafficking of ciliary G protein-coupled receptors; INTRODUCTION | |
| 505 | 8 | |a 1. RHODOPSIN AS A CILIARY GPCR2. CILIARY GPCRS IN OTHER NEURONS; 3. OLFACTORY SENSORY NEURON CILIARY GPCRS; 4. DYNAMIC TRAFFICKING OF CILIARY GPCRS; CONCLUSIONS AND FUTURE DIRECTIONS; REFERENCES; 4 -- Single-molecule resolution of G protein-coupled receptor (GPCR) complexes; INTRODUCTION; 1. IMAGING METHODOLOGIES TO STUDY SINGLE GPCR MOLECULES; 1.1 Current Approaches to Visualize Individual GPCRs as Monomers, Dimers, and Oligomers; 1.2 Super-Resolution Imaging of Single Molecules: Resolving Lots and Lots of Spots ... ; 2. METHODS FOR PHOTOACTIVATED DYE-LOCALIZATION MICROSCOPY | |
| 505 | 8 | |a 2.1 Seeing Single Molecules2.1.1 Choice of probe and labeling; 2.1.2 Optimization steps-labeling cells, blocking buffers, and fixatives; 2.1.3 Imaging considerations and conditions; 2.2 Counting Single Molecules; 2.2.1 Resolving single-molecule data; 2.2.2 Custom quantification of GPCR monomers, dimers, and oligomers; CONCLUSIONS AND PERSPECTIVES; Acknowledgments; REFERENCES; 5 -- Quantification of the mRNA expression of G protein-coupled receptors in human adipose tissue; INTRODUCTION; 1. MATERIALS; 1.1 Handling of Adipose Tissue Biopsies; 1.2 Adipose Tissue Homogenization | |
| 505 | 8 | |a 1.3 Adipose Tissue RNA Purification and cDNA Generation1.4 qPCR Quantification of GPCR Expression Relative the Adipose Tissue Housekeeping Gene PPIA; 2. METHODS; 2.1 Handling of Adipose Tissue Biopsies; 2.2 Adipose Tissue Homogenization; 2.3 Adipose Tissue RNA Purification and cDNA Generation; 2.4 qPCR Quantification of GPCR Expression Relative to the Housekeeping Gene PPIA; 2.4.1 Screening of adipose tissue GPCR mRNA expression using pooled cDNA; 2.4.2 Quantification of GPCR expression relative to the expression of the adipose tissue housekeeping gene PPIA | |
| 588 | 0 | |a Online resource; title from digital title page (viewed on March 26, 2018). | |
| 650 | 0 | |a G proteins |x Receptors. | |
| 650 | 0 | |a Cytology |x Technique. | |
| 650 | 0 | |a Cell receptors. | |
| 650 | 0 | |a G proteins. | |
| 650 | 1 | 2 | |a Cytological Techniques |0 (DNLM)D003584 |
| 650 | 1 | 2 | |a GTP-Binding Proteins |0 (DNLM)D019204 |
| 650 | 1 | 2 | |a Receptors, Cell Surface |0 (DNLM)D011956 |
| 650 | 6 | |a Prot�eines G |0 (CaQQLa)201-0203891 |x R�ecepteurs. |0 (CaQQLa)201-0375415 | |
| 650 | 6 | |a Cytologie |x Technique. |0 (CaQQLa)201-0005040 | |
| 650 | 6 | |a R�ecepteurs cellulaires. |0 (CaQQLa)201-0010863 | |
| 650 | 6 | |a Prot�eines G. |0 (CaQQLa)201-0203891 | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Anatomy & Physiology. |2 bisacsh | |
| 650 | 7 | |a G proteins |2 fast |0 (OCoLC)fst00936830 | |
| 650 | 7 | |a G proteins |x Receptors |2 fast |0 (OCoLC)fst00936832 | |
| 650 | 7 | |a Cell receptors |2 fast |0 (OCoLC)fst00850227 | |
| 650 | 7 | |a Cytology |x Technique |2 fast |0 (OCoLC)fst00886306 | |
| 655 | 4 | |a Internet Resources. | |
| 700 | 1 | |a Shukla, Arun K., |e editor. | |
| 776 | 0 | 8 | |i Print version: |a Shukla, Arun K. |t G Protein-Coupled Receptors: Signaling, Trafficking and Regulation. |d : Elsevier Science, �2016 |z 9780128035955 |
| 830 | 0 | |a Methods in cell biology ; |v v. 132. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780128035955 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/publication?issn=0091679X&volume=132 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/0091679X/132 |z Texto completo |