Cargando…
Intermediate filament associated proteins /
Intermediate Filament Associated Proteins, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in intermediate filament associated proteins and contains sections...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Waltham, MA :
Academic Press, an imprint of Elsevier,
2016.
|
| Edición: | First edition. |
| Colección: | Methods in enzymology ;
v. 569. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
MARC
| LEADER | 00000cam a2200000Ii 4500 | ||
|---|---|---|---|
| 001 | SCIDIR_ocn935112541 | ||
| 003 | OCoLC | ||
| 005 | 20231120112047.0 | ||
| 006 | m o d | ||
| 007 | cr cnu---unuuu | ||
| 008 | 160118s2016 maua ob 001 0 eng d | ||
| 040 | |a N$T |b eng |e rda |e pn |c N$T |d N$T |d BUF |d IDEBK |d EBLCP |d YDXCP |d OPELS |d OCLCF |d CDX |d OCLCQ |d CUS |d OCLCQ |d MERUC |d UWW |d FEM |d AU@ |d OCLCQ |d SOI |d OCLCQ |d OCLCO |d UKMGB |d OCLCQ |d OCLCA |d REDDC |d VT2 |d OCLCQ |d OCLCO |d OCLCQ |d CASUM |d OCLCO | ||
| 015 | |a GBB604331 |2 bnb | ||
| 016 | 7 | |a 017695905 |2 Uk | |
| 019 | |a 968987678 |a 1229064196 |a 1232395372 |a 1235828038 |a 1257366239 |a 1259072309 | ||
| 020 | |a 9780128034903 |q (electronic bk.) | ||
| 020 | |a 0128034904 |q (electronic bk.) | ||
| 020 | |a 0128034696 | ||
| 020 | |a 9780128034699 | ||
| 020 | |z 9780128034699 | ||
| 035 | |a (OCoLC)935112541 |z (OCoLC)968987678 |z (OCoLC)1229064196 |z (OCoLC)1232395372 |z (OCoLC)1235828038 |z (OCoLC)1257366239 |z (OCoLC)1259072309 | ||
| 050 | 4 | |a QD431 | |
| 072 | 7 | |a SCI |x 007000 |2 bisacsh | |
| 082 | 0 | 4 | |a 572.6 |2 23 |
| 245 | 0 | 0 | |a Intermediate filament associated proteins / |c edited by Katherine L. Wilson, Arnoud Sonnenberg. |
| 250 | |a First edition. | ||
| 264 | 1 | |a Waltham, MA : |b Academic Press, an imprint of Elsevier, |c 2016. | |
| 300 | |a 1 online resource : |b illustrations (chiefly color) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a data file | ||
| 490 | 1 | |a Methods in enzymology, |x 0076-6879 ; |v volume 569 | |
| 588 | 0 | |a Online resource; title from PDF title page (EBSCO, viewed January 20, 2016). | |
| 504 | |a Includes bibliographical references and indexes. | ||
| 520 | |a Intermediate Filament Associated Proteins, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in intermediate filament associated proteins and contains sections on such topics as lamin-associated proteins, intermediate filament-associated proteins and plakin, and other cytoskeletal cross-linkers. | ||
| 505 | 0 | |a Front Cover -- Intermediate Filament Associated Proteins -- Copyright -- Contents -- Contributors -- Preface -- Part I: Identification and Biochemical Analysis of Lamin-Associated Proteins -- Chapter One: BioID Identification of Lamin-Associated Proteins -- 1. Introduction -- 1.1. Nuclear Lamina -- 1.2. Protein-Protein Interactions -- 1.3. BioID -- 2. BioID Method -- 2.1. Construction of a BioID-Fusion Protein -- 2.2. Cell Choice -- 2.3. Functional Assessment of the Fusion Protein -- 2.4. Establishing a Stable BioID Cell Line -- 2.4.1. Materials. | |
| 505 | 8 | |a 2.4.2. Generation and Validation of BioID-Fusion Protein -- 2.4.3. Generate Cells That Stably Express the BioID-Fusion Protein -- 2.5. Large-Scale BioID Pull-Down to Identify Interacting Proteins -- 2.5.1. Materials -- 2.5.2. Cell Lysis -- 2.5.3. Affinity Purification of Biotinylated Proteins -- 2.6. Reagents and Solutions -- 3. Anticipated Results -- 4. Conclusions -- Acknowledgment -- References -- Chapter Two: Proximity-Dependent Biotin Identification (BioID) in Dictyostelium Amoebae -- 1. Introduction -- 1.1. Lamins and Their Partners: The Challenge -- 1.2. Advantages and Limitations of BioID. | |
| 505 | 8 | |a 1.2.1. Limitations of BioID -- 1.3. Adaptation of BioID to the Dictyostelium System -- 1.4. Overview of BioID Protocol in Dictyostelium -- 2. Materials -- 2.1. Materials for Strain Generation -- 2.2. Materials for Cell Growth and Isolation of Nuclei -- 2.2.1. Cell Preparation -- 2.2.2. Cell Lysis and Isolation of Nuclei -- 2.2.3. Solubilization of Nuclear Material and Isolation of Biotinylated Proteins -- 2.3. Materials for Light Microscopy -- 3. Methods -- 3.1. Generate Strains Expressing the BirA-R118G-Fused Protein of Interest -- 3.1.1. Cell Growth and Electrotransformation. | |
| 505 | 8 | |a 3.1.2. Clonal Selection with Blasticidin S -- 3.1.3. Clonal Selection with G418 -- 3.2. Isolate Nuclei, Prepare Lysates, and Purify Biotinylated Proteins -- 3.2.1. Cell Preparation -- 3.2.2. Cell Lysis and Isolation of Nuclei -- 3.2.3. Solubilization of Nuclei and Isolation of Biotinylated Proteins -- 3.3. Light Microscopy -- 3.3.1. Fixation and Fluorescence Microscopy of Whole Cells -- 3.3.2. Fixation and Fluorescence Microscopy of Isolated Nuclei -- 3.4. Electrophoresis and Immunoblotting -- 4. Conclusions -- References. | |
| 505 | 8 | |a Chapter Three: Purification and Structural Analysis of LEM-Domain Proteins -- 1. Introduction -- 2. ��Divide and Conquer�� Approach to Study LEM-Domain Proteins -- 2.1. The LEM Domain Common to LAP2, Emerin, and MAN1 -- 2.2. The C-Terminal Domain Specific to LAP2� -- 2.3. The WH and UHM Domains in MAN1 -- 3. Analysis of Predicted Unstructured Regions in LEM-Domain Proteins -- 3.1. Production and Purification of Predicted Unstructured Regions of Emerin and MAN1 -- 3.1.1. Emerin Nucleoplasmic Region -- 3.1.2. The N-Terminal Nucleoplasmic Region of MAN1. | |
| 650 | 0 | |a Proteins. | |
| 650 | 0 | |a Intermediate filament proteins. | |
| 650 | 2 | |a Intermediate Filament Proteins |0 (DNLM)D007381 | |
| 650 | 2 | |a Proteins |0 (DNLM)D011506 | |
| 650 | 6 | |a Prot�eines. |0 (CaQQLa)201-0008150 | |
| 650 | 6 | |a Prot�eines des filaments interm�ediaires. |0 (CaQQLa)201-0162956 | |
| 650 | 7 | |a protein. |2 aat |0 (CStmoGRI)aat300206575 | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Biochemistry. |2 bisacsh | |
| 650 | 7 | |a Intermediate filament proteins |2 fast |0 (OCoLC)fst00976468 | |
| 650 | 7 | |a Proteins |2 fast |0 (OCoLC)fst01079711 | |
| 655 | 4 | |a Internet Resources. | |
| 700 | 1 | |a Wilson, Katherine L., |e editor. | |
| 700 | 1 | |a Sonnenberg, Arnoud, |e editor. | |
| 776 | 0 | 8 | |i Print version : |z 9780128034699 |
| 830 | 0 | |a Methods in enzymology ; |v v. 569. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/569 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780128034699 |z Texto completo |