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Calorimetry /
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Cambridge, MA :
Academic Press,
2016.
|
| Edición: | First edition. |
| Colección: | Methods in enzymology ;
v. 567. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- 6. Additional Considerations with Metal Ions6.1. Comparison with Other Methods; 6.2. Sources of Error; 6.3. Tips; 7. Conclusion; Acknowledgments; References; Chapter Two: Assessing Coupled Protein Folding and Binding Through Temperature-Dependent Isothermal Titration Calorimetry; 1. Introduction; 2. Sample Preparation; 2.1. Plasmid Construction; 2.2. Purification of FCP1 and Rap74; 3. Special Considerations for ITC of Intrinsically Disordered Proteins; 3.1. Experimental Design; 3.2. Calorimetric Parameters; 4. Data Fitting and Analysis; 4.1. Standard Data Fitting in Origin
- 4.2. Data Fitting in MATLAB4.3. Global Analysis of Temperature-Dependent Data Sets; 5. Summary; Acknowledgments; References; Chapter Three: Fragment-Based Screening for Enzyme Inhibitors Using Calorimetry; 1. Introduction; 1.1. Nanocalorimeter Arrays; 1.2. Fragment-Based Lead Discovery; 2. Required Materials; 2.1. Equipment; 2.2. Reagents; 2.3. Software; 3. Instrumentation; 4. Fragment Library Selection; 4.1. Materials Needed; 4.2. Software Needed; 4.3. Protocol to Prepare of Fragment Library for Screening; 5. Calorimetric Enzyme Assay: Nanocalorimeter
- 5.1. Sample Considerations and Preparation5.2. Data Analysis; 5.3. Protocol; 6. Calorimetric Enzyme Assay: Commercial Microcalorimeters; 6.1. Required Materials; 6.2. Cleaning Before and After Runs; 6.3. Protocol 2: Microcalorimeter; 6.4. Data Processing and Analysis; 7. Summary; Acknowledgments; References; Chapter Four: Measuring Multivalent Binding Interactions by Isothermal Titration Calorimetry; 1. Introduction; 2. Determining the Thermodynamics of Lectin Binding to Multivalent Synthetic Analogs; 2.1. Methods; 2.1.1. Isothermal Titration Calorimetry