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Calorimetry /
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Cambridge, MA :
Academic Press,
2016.
|
| Edición: | First edition. |
| Colección: | Methods in enzymology ;
v. 567. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
MARC
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| 082 | 0 | 4 | |a 536.6 |2 23 |
| 245 | 0 | 0 | |a Calorimetry / |c edited by Andrew L. Feig. |
| 250 | |a First edition. | ||
| 264 | 1 | |a Cambridge, MA : |b Academic Press, |c 2016. | |
| 264 | 4 | |c �2016 | |
| 300 | |a 1 online resource (xix, 493 pages) : |b illustrations (chiefly color) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a data file | ||
| 490 | 1 | |a Methods in enzymology, |x 0076-6879 ; |v volume 567 | |
| 504 | |a Includes bibliographical references and indexes. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 8 | |6 880-01 |a 6. Additional Considerations with Metal Ions6.1. Comparison with Other Methods; 6.2. Sources of Error; 6.3. Tips; 7. Conclusion; Acknowledgments; References; Chapter Two: Assessing Coupled Protein Folding and Binding Through Temperature-Dependent Isothermal Titration Calorimetry; 1. Introduction; 2. Sample Preparation; 2.1. Plasmid Construction; 2.2. Purification of FCP1 and Rap74; 3. Special Considerations for ITC of Intrinsically Disordered Proteins; 3.1. Experimental Design; 3.2. Calorimetric Parameters; 4. Data Fitting and Analysis; 4.1. Standard Data Fitting in Origin | |
| 505 | 8 | |a 4.2. Data Fitting in MATLAB4.3. Global Analysis of Temperature-Dependent Data Sets; 5. Summary; Acknowledgments; References; Chapter Three: Fragment-Based Screening for Enzyme Inhibitors Using Calorimetry; 1. Introduction; 1.1. Nanocalorimeter Arrays; 1.2. Fragment-Based Lead Discovery; 2. Required Materials; 2.1. Equipment; 2.2. Reagents; 2.3. Software; 3. Instrumentation; 4. Fragment Library Selection; 4.1. Materials Needed; 4.2. Software Needed; 4.3. Protocol to Prepare of Fragment Library for Screening; 5. Calorimetric Enzyme Assay: Nanocalorimeter | |
| 505 | 8 | |a 5.1. Sample Considerations and Preparation5.2. Data Analysis; 5.3. Protocol; 6. Calorimetric Enzyme Assay: Commercial Microcalorimeters; 6.1. Required Materials; 6.2. Cleaning Before and After Runs; 6.3. Protocol 2: Microcalorimeter; 6.4. Data Processing and Analysis; 7. Summary; Acknowledgments; References; Chapter Four: Measuring Multivalent Binding Interactions by Isothermal Titration Calorimetry; 1. Introduction; 2. Determining the Thermodynamics of Lectin Binding to Multivalent Synthetic Analogs; 2.1. Methods; 2.1.1. Isothermal Titration Calorimetry | |
| 650 | 0 | |a Calorimetry. | |
| 650 | 1 | 2 | |a Calorimetry |0 (DNLM)D002151 |
| 650 | 6 | |a Calorim�etrie. |0 (CaQQLa)201-0011344 | |
| 650 | 7 | |a calorimetry. |2 aat |0 (CStmoGRI)aat300214718 | |
| 650 | 7 | |a SCIENCE |x Mechanics |x Thermodynamics. |2 bisacsh | |
| 650 | 7 | |a Calorimetry |2 fast |0 (OCoLC)fst00844526 | |
| 655 | 4 | |a Internet Resources. | |
| 700 | 1 | |a Feig, Andrew L., |e editor. | |
| 776 | 0 | 8 | |i Print version: |a Feig, Andrew. |t Calorimetry. |d : Elsevier Science, �2016 |z 9780128029060 |
| 830 | 0 | |a Methods in enzymology ; |v v. 567. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780128029060 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/567 |z Texto completo |
| 880 | 8 | |6 505-00/(S |a 2.1.2. Determination of Functional Valence of Multivalent Ligands2.2. Data Analysis and Interpretation; 2.2.1. ITC can Reliably Measure the Enhanced Affinities of Multivalent Carbohydrates; 2.2.2. Information Obtained from ITC-Driven n Values; 2.2.3. Structural Valence of a Multivalent Ligand May Differ from Its Functional Valence; 2.2.4. Macroscopic-Binding Enthalpy of Multivalent Carbohydrate Analogs Is the sum of the Microscopic Enthalpy of the Epi ... ; 2.2.5. Macroscopic TΔS of High-Affinity Carbohydrate Analogs Is More Than the Sum of Microscopic TΔS | |
| 880 | 0 | |6 505-01/(S |a Front Cover; Calorimetry; Copyright; Contents; Contributors; Preface; References; Section I: ITC; Chapter One: Isothermal Titration Calorimetry Measurements of Metal Ions Binding to Proteins; 1. Introduction; 2. Metal Ion Properties Relevant to ITC Measurements; 2.1. Aqueous Solution Chemistry of Metal Ions; 2.2. Buffers; 2.3. Protons; 3. ITC Measurements Involving Metal Ions; 4. Fitting ITC Data Involving Metal Ions; 5. Post Hoc Analysis of ITC Data Involving Metal Ions; 5.1. Binding Enthalpy (ΔH); 5.2. Equilibrium Constant (K); 5.3. Binding Entropy (ΔS) and Heat Capacity (ΔCP) | |