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Polymers and nanomaterials for gene therapy /
Polymers and Nanomaterials for Gene Therapy provides the latest information on gene therapy, a topic that has attracted significant attention over the past two decades for the treatment of inherited and acquired genetic diseases. Major research efforts are currently focused on designing suitable car...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Waltham, MA :
Woodhead Publishing,
[2016]
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| Colección: | Woodhead Publishing series in biomedicine ;
no. 83. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- PrefaceChapter 1: Mechanism, current challenges and new approaches for non viral gene delivery; 1.1 Introduction; 1.2 Current non-viral gene delivery vectors; 1.2.1 Naked DNA; 1.2.2 Cationic lipids; 1.2.2.1 Liposomes; 1.2.2.2 Lipid-coated DNA complexes; 1.2.2.3 Niosomes; 1.2.3 Cationic polymers; 1.2.3.1 Polyethylenimine (PEI); 1.2.3.2 Poly-L-lysine (PLL); 1.2.3.3 Polymethacrylate; 1.2.3.4 Chitosan; 1.2.3.5 Dendrimers; 1.2.3.5.1 Polyamidoamine dendrimers (PAMAM); 1.2.3.5.2 Poly(propylenimine) dendrimers (PPI); 1.2.3.5.3 Poly(L-lysine) dendrimers
- 1.3 Trajectory and mechanism of non-viral vectors1.3.1 Basics of non-viral gene delivery system formulation; 1.3.1.1 Complexes between cationic lipid and DNA (lipoplexes); 1.3.1.2 Complexes between cationic polymer and DNA (polyplexes); 1.3.2 Binding and uptake; 1.3.2.1 Phagocytosis; 1.3.2.2 Clathrin-mediated endocytosis; 1.3.2.3 Caveolae-mediated endocytosis; 1.3.2.4 Macropinocytosis; 1.3.2.5 Non-endocytic pathways; 1.3.3 Characteristics of non-viral gene delivery influencing uptake pathways; 1.3.3.1 Size; 1.3.3.2 Surface charge; 1.3.3.3 Shape; 1.3.3.4 Cell type
- 1.3.4 Endosomal escape and dissociation of complexes1.3.4.1 Lipoplexes; 1.3.4.2 Polyplexes; 1.3.5 Fate in cytoplasm and nuclear entry; 1.4 Conclusion and future perspective; References; Chapter 2: PEI and its derivatives for gene therapy; 2.1 Introduction; 2.2 Criteria for non-viral vectors and general considerations; 2.3 Rationale for the use of PEI for gene therapy; 2.4 PEI Derivatives for gene delivery; 2.4.1 Grafting targeting ligands on PEI; 2.4.2 Chemical modification to shield cationic charge density; 2.4.3 Hydrophobic modifications; 2.4.4 Stimuli-sensitive construct
- 2.4.4.1 Redox-sensitive construct2.4.5 pH-sensitive PEI constructs; 2.4.6 Temperature-sensitive PEI constructs; 2.5 PEGylation of PEI; 2.6 Polyester conjugation on PEI; 2.7 Chitosan conjugation on PEI; 2.8 PLL conjugation on PEI; 2.9 Metallic nanoparticles with PEIs; 2.10 Pharmacokinetics and bio-distribution of PEI delivery vectors; 2.11 Concluding remarks; Acknowledgements; References; Chapter 3: Natural polymers in nucleic acid delivery; 3.1 Introducing cells to exogenous nucleic acids; 3.1.1 Nucleic acids as therapeutics; 3.1.2 Applications in cancer