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Introduction to protein mass spectrometry /

Unlike many other methods which automatically yield an absolutely unique protein name as output, protein mass spectrometry generally requires a deduction of protein identity from determination of peptide fragmentation products. This book enables readers to both understand, and appreciate, how determ...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Ghosh, Pradip K. (Autor)
Formato: Electrónico eBook
Idioma:Inglés
Publicado: London, UK : Academic Press is an imprint of Elsevier, [2015]
Temas:
Acceso en línea:Texto completo

MARC

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100 1 |a Ghosh, Pradip K.,  |e author. 
245 1 0 |a Introduction to protein mass spectrometry /  |c Pradip Kumar Ghosh. 
264 1 |a London, UK :  |b Academic Press is an imprint of Elsevier,  |c [2015] 
264 4 |c �2016 
300 |a 1 online resource 
336 |a text  |b txt  |2 rdacontent 
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500 |a Includes index. 
588 0 |a Online resource; title from PDF title page (ScienceDirect, viewed December 15, 2015). 
505 0 |a Title page; Table of Contents; Copyright page; Dedication; Preface; Chapter 1: Introduction; Chapter 2: Sample Preparation and Ionization for Mass Spectrometry; 2.1 Separation of protein mixtures; 2.2 Multidimensional separations; 2.3 Proteolysis; 2.4 Ionization of proteins and peptides; Chapter 3: Instruments; 3.1 Mass analysis; 3.2 Some examples of instruments; Chapter 4: Mass Spectrometry of Peptides and Proteins; 4.1 Proteins; 4.2 Alternative analysis methods; 4.3 Quantitation; 4.4 Peptide fragmentation: Experiments; 4.5 Data mining scheme for identifying peptide structural motifs. 
505 8 |a 4.6 Peptide fragmentation: mechanism4.7 Possible pathways in the competition model; 4.8 Studying post translational modifications; 4.9 Chemical cross-linking/mass spectrometry; 4.10 Manual de novo sequencing of peptides; Chapter 5: Examples from Biological Applications; 5.1 Mapping intact protein isoforms using top-down proteomics; 5.2 Quantitative analysis of intact apolipoproteins in human HDL; 5.3 Rapid sequence analysis of some conotoxins -- combination of de novo, bottom-up methods; 5.4 Mass spectrometry of ribosomes; 5.5 Proteins in Purkinje cell post-synaptic densities. 
505 8 |a 5.6 Neurexin-LRRTM2 interaction effect in synapse formation5.7 Rapid analysis of human plasma proteome -- an IMS-IMS-MS application; 5.8 Topology of two transient virus capsid assembly intermediates; 5.9 Mass spectrometry of intact V-type ATPases reveals bound lipids and the effects of nucleotide binding; 5.10 Mass spectrometric imaging of biological material; Chapter 6: Mass Spectrometry-Based Bioinformatics; 6.1 Peptides to proteins; 6.2 MS/MS fragments to peptides to proteins; 6.3 Data-dependent acquisition; 6.4 Targeted acquisition covering SRM and PRM; 6.5 Data-independent acquisition. 
520 8 |a Unlike many other methods which automatically yield an absolutely unique protein name as output, protein mass spectrometry generally requires a deduction of protein identity from determination of peptide fragmentation products. This book enables readers to both understand, and appreciate, how determinations about protein identity from mass spectrometric data are made. 
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650 7 |a Mass spectrometry  |2 fast  |0 (OCoLC)fst01011435 
776 0 8 |i Print version:  |a Ghosh, Pradip Kumar.  |t Introduction to Protein Mass Spectrometry.  |d : Elsevier Science, �2015  |z 9780128051238 
856 4 0 |u https://sciencedirect.uam.elogim.com/science/book/9780128021026  |z Texto completo