Isotope labeling of biomolecules : labeling methods /

Isotope Labeling of Biomolecules - Labeling Methods, the latest volume of the Methods in Enzymology series contains comprehensive information on stable isotope labeling methods and applications for biomolecules.

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Kelman, Zvi (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam, Netherlands : Elsevier/Academic Press, 2015.
Colección:Methods in enzymology ; v. 565.
Temas:
Biomolecules > Analysis.
Stable isotope tracers.
Isotope Labeling > methods
Biomol�ecules > Analyse.
Isotopes stables.
MEDICAL > Pharmacology.
Biomolecules > Analysis
Stable isotope tracers
Internet Resources.
Acceso en línea:Texto completo
Texto completo
Tabla de Contenidos:
  • Robust high-yield methodologies for �H and �H/15N/13C labeling of proteins for structural investigations using neutron scattering and NMR
  • Protein labeling in Escherichia coli with �H, 13C, and 15N
  • Escherichia coli auxotroph host strains for amino acid-selective isotope labeling of recombinant proteins
  • 19F-modified proteins and 19F-containing ligands as tools in solution NMR studies of protein interactions
  • Biopolymer deuteration for neutron scattering and other isotope-sensitive techniques
  • Production of bacterial cellulose with controlled deuterium-hydrogen substitution for neutron scattering studies
  • Isotope labeling of proteins in Halobacterium salinarum
  • Amino acid selective unlabeling in protein NMR spectroscopy
  • Isotope labeling of eukaryotic membrane proteins in yeast for solid-state NMR
  • Development of approaches for deuterium incorporation in plants
  • Isotope labeling of proteins in insect cells
  • Effective isotope labeling of proteins in a mammalian expression system
  • Eschericia coli cell-free synthesis and isotope labeling of mammalian proteins
  • Rapid biosynthesis of stable isotope-labeled peptides from a reconstituted in vitro translation system for targeted proteomics 347
  • Labeling of membrane proteins by cell-free expression
  • Selective amino acid segmental labeling of multi-domain proteins
  • Labeling monosaccharides with stable isotopes
  • Stable isotope-labeled RNA phosphoramidites to facilitate dynamics by NMR
  • In vivo, large-scale preparation of uniformly 15N- and site-specifically 13C-labeled homogrenous, recombinant RNA for NMR studies
  • Cut and paste RNA for nuclear magentic resonance, paramagnetic resonance enhancement, and electron paramagnetic resonance structural studies.

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