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Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions. Part A /
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam, Netherlands :
Academic Press is an imprint of Elsevier,
2015.
|
| Colección: | Methods in enzymology ;
v. 563. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- Title page; Table of Contents; Copyright; Contributors; Preface; Section I: EPR Methodology and Instrumentation; Chapter One: Rapid-Scan EPR of Nitroxide Spin Labels and Semiquinones; Abstract; 1 Introduction; 2 Rapid-Scan Method; 3 Why Rapid Scan Improves S/N; 4 Other Advantages of Rapid Scan; 5 Hardware Requirements; 6 Parameter Selection; 7 Examples; 8 Wider Scans; 9 Future Applications; Acknowledgments; Chapter Two: Fourier Deconvolution Methods for Resolution Enhancement in Continuous-Wave EPR Spectroscopy; Abstract; 1 Introduction; 2 Historical Perspective; 3 Overlapping Signals
- 4 Fourier Filtering5 Overview of Method; 6 Practical Examples; 7 Limitations of the Method; 8 Summary; Acknowledgment; Supplementary materials; Chapter Three: Multifrequency Pulsed EPR and the Characterization of Molecular Dynamics; Abstract; 1 Introduction; 2 Contributions to Electron Spin-Lattice Relaxation and Dependence of Relaxation on Motions in Fluid Solution; 3 Measurement of Spin-Lattice Relaxation, T1; 4 Examples of Motions Elucidated by Measuring T1 as a Function of Frequency; 5 Conclusion; Acknowledgments
- Chapter Four: Resolution and Characterization of Chemical Steps in Enzyme Catalytic Sequences by Using Low-Temperature and Time-Resolved, Full-Spectrum EPR Spectroscopy in Fluid Cryosolvent and Frozen Solution SystemsAbstract; 1 Introduction; 2 Low-Temperature Fluid Cryosolvent System; 3 Low-Temperature Frozen Solution System; 4 Analysis and Interpretations: General Cases and Guidelines; 5 Conclusions; Acknowledgments; Chapter Five: 230/115 GHz Electron Paramagnetic Resonance/Double Electron-Electron Resonance Spectroscopy; Abstract; 1 Introduction; 2 HF EPR/DEER Spectrometer
- 3 Examples of HF EPR: Paramagnetic Impurities in Diamonds Studied by 230 GHz EPR/DEER Spectroscopy4 Summary; Acknowledgments; Appendix; Section II: EPR Spectral Simulation and Software; Chapter Six: CW-EPR Spectral Simulations: Solid State; Abstract; 1 Introduction; 2 Spins and SH; 3 Dynamic Regime; 4 Levels of Theory; 5 Orientational Order and Disorder; 6 Structural Order and Disorder; 7 Other Line Broadenings; 8 Experimental Effects; 9 Fitting; 10 Conclusions; Chapter Seven: CW-EPR Spectral Simulations: Slow-Motion Regime; Abstract; 1 Introduction; 2 Magnetic Parameters
- 3 Methods of Modeling Slow Motion4 Outlook; Acknowledgment; Chapter Eight: Quantitative Interpretation of Multifrequency Multimode EPR Spectra of Metal Containing Proteins, Enzymes, and Biomimetic Complexes; Abstract; 1 Introduction; 2 Basic EPR Theory; 3 g-Factor Anisotropy; 4 Hyperfine Structure; 5 Spin Hamiltonian; 6 Basic EPR Instrumentation; 7 Simulation of Powder Spectra; 8 Quantitative Aspects; 9 Applications; 10 Conclusion; Acknowledgments; Section III: EPR Studies of Metal-Containing Proteinsand Metal Cofactors