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Molecular microbial diagnostic methods : pathways to implementation for the food and water industries /
Molecular Microbial Diagnostic Methods: Pathways to Implementation in the Food and Water Industries attempts to address the shortage of guidance on implementation of molecular-based methods for routine diagnostic laboratories. What industry and analysts can expect from routine use of these methods i...
| Clasificación: | Libro Electrónico |
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| Otros Autores: | , , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
London, UK :
Academic Press is an imprint of Elsevier,
[2015]
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Title page; Table of Contents; Copyright; Contributors; Preface; Chapter 1: Food industry current status; Abstract; Introduction; Molecular methods currently used; Laboratory automation in molecular methods; Challenges; Acceptance of use by the food industry; Compliance with current legal requirements; Disclaimer; Chapter 2: Future directions for molecular microbial diagnostic methods for the food industry; Abstract; Introduction; Evolution of food microbiology diagnostics: from petri dishes to PCR; Why introduce an alternative molecular diagnostic method?
- Characteristics of an ideal food molecular methodPast and current challenges; Current challenges; Concluding remarks; Chapter 3: Current status of molecular microbiological techniques for the analysis of drinking water; Abstract; Introduction and overview; The current state of play; The influence of standard and reference methods; Molecular techniques for testing potable water quality; Application of molecular techniques to wastewater; Conclusions; Chapter 4: What is now required for water?; Abstract; Introduction and overview; Indicator organisms; Pathogens in drinking water
- Total heterotrophic bacteriaFeasibility of the use of routine rapid water molecular methods; Future predictions; Conclusions; Chapter 5: CEN/ISO standards for both culture and molecular methods; Abstract; Introduction; Standards, standardization bodies, and structures in the microbiology of the food chain; Standards developed in the microbiology of the food chain; Status of novel technologies; Conclusions; Chapter 6: Laboratory validation, verification, and accreditation of molecular methods; Abstract; Alternative methods; Foundations of an accredited laboratory; Quality systems
- Cornerstones of accreditationMaintaining accreditation; Customer education; Health and safety; Chapter 7: DNA extraction: finding the most suitable method; Abstract; Boiling method; Column extraction; Magnetic beads; FTA" cards; RNA extraction methods; Conclusions; Chapter 8: Assessing organism viability and interpreting genomic unit versus colony forming unit data for water and food borne microorganisms, such as Legionella, Campylobacter, Salmonella, and Listeria; Abstract; Introduction; Polymerase chain reaction; Assessing microbial viability; Overcoming the viability hurdle
- When to use viability discriminationStandardization and quality assurance; Proficiency testing; Conclusions; Chapter 9: MALDI-TOF MS: a rapid microbiological confirmation technique for food and water analysis; Abstract; Introduction and overview; Current confirmation methods and advantages of MALDI-TOF MS; MALDI-TOF MS Listeria speciation validation; Chapter 10: Chapter highlights, future requirements, and conclusions; Chapter highlights; Future requirements and conclusions; Index